Development of the functional mutagenesis system on Plasmodium prarasite

2013 Fiscal Year Final Research Report

• Project/Area Number: 23658235
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUMOTO Shinya 帯広畜産大学, 原虫病研究センター, 准教授 (50376422)
• Project Period (FY): 2011 – 2012
• Keywords: マラリア / ベクター

Research Abstract

Genetic mutant is a powerful tool for the infectious disease research. Thus, the development of mutant screening system in the parasitic disease in veterinary field is desired. In this study, we tried to develop the transposon based random mutant screening system using murine malaria parasite as a model pathogen. As a result, we successfully established the gain-of function mutant parasite library system. Furthermore, with this system, we successfully could identify the genomic locus that highly concerns to vector transmission.

Research Products

• [Journal Article] Multivariable analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium yoelii (2013)
  • Author(s): Saiki, E., Nagao, K., Aonuma, H., Fukumoto, S., Xuan, X., Bannai, M.Kanuka, H
  • Journal Title: Malar J Volume: 12 Pages: 19
  • DOI: 10.1186/1475-2875-12-19
  • Peer Reviewed
• [Journal Article] Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity (2013)
  • Author(s): Bando, H., Okado, K., Guelbeogo, W. M., Badolo, A., Aonuma, H., Nelson, B., Fukumoto, S., Xuan, X., Sagnon, N.Kanuka, H
  • Journal Title: Sci Rep Volume: 3 Pages: 1641
  • DOI: 10.1038/srep01641
  • Peer Reviewed
• [Journal Article] Mitochondrial peroxidase TPx-2 is not essential in the blood and insect stages of Plasmodium berghei (2012)
  • Author(s): Masuda-Suganuma, H., Usui, M., Fukumoto, S., Inoue, N.Kawazu, S
  • Journal Title: Parasit Vectors Volume: 5 Pages: 252
  • DOI: 10.1186/1756-3305-5-252
  • Peer Reviewed
• [Journal Article] Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s. 1 (2012)
  • Author(s): Badolo, A., Okado, K., Guelbeogo, W. M., Aonuma, H., Bando, H., Fukumoto, S., Sagnon, N.Kanuka, H
  • Journal Title: Malar J Volume: 11 Pages: 227
  • DOI: 10.1186/1475-2875-11-227
  • Peer Reviewed
• [Journal Article] In vitro and in vivo antimalarial activities of T-2307, a novel arylamidine (2012)
  • Author(s): Kimura, A., Nishikawa, H., Nomura, N., Mitsuyama, J., Fukumoto, S., Inoue, N.Kawazu, S
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Pages: 2191-3
  • DOI: 10.1128/AAC.05856-11
  • Peer Reviewed
• [Journal Article] Calcium signal regulates temperature-dependent transformation of sporozoites in malaria parasite development (2011)
  • Author(s): Doi, Y., Shinzawa, N., Fukumoto, S., Okano, H.Kanuka, H
  • Journal Title: Exp Parasitol Volume: 128 Pages: 176-80
  • DOI: 10.1016/j.exppara.2011.02.011
  • Peer Reviewed
• [Journal Article] Improvement of the observational method for Plasmodium berghei oocysts in the midgut of mosquitoes (2011)
  • Author(s): Usui, M., Fukumoto, S., Inoue, N.Kawazu, S
  • Journal Title: Parasit Vectors Volume: 4 Pages: 118
  • DOI: 10.1186/1756-3305-4-118
  • Peer Reviewed
• [Presentation] Amino acid-related host nutrition dynamics during malaria infection (2013)
  • Author(s): Saiki E, Nagao K, Fukumoto S, Bannai M, Kanuka H
  • Organizer: Keystone Symposia on Molecular and Cellular Biology : Malaria
  • Place of Presentation: JW Marriott New Orleans, New Orleans, Louisiana USA
  • Year and Date: 20130120-25
• [Presentation] Impact of intra-specific diversity of mosquito midgut bacteria on Plasmodium development (2011)
  • Author(s): Hironori Bando, Kiyoshi Okado, Moussa Guelbeogo, Athanase Badolo, Hiroka Aonuma, Shinya Fukumoto, N'Fale Sagnon, and Hirotaka Kanuka
  • Organizer: 第80回米国熱帯医学会
  • Place of Presentation: フィラデルフィア・ペンシルバニア
  • Year and Date: 20111204-08
• [Presentation] Development of allele-specific loop-mediated isothermal amplification method (AS-LAMP) for detection of the L1104Fkdr- mutation in Anopheles gambiae s. 1 (2011)
  • Author(s): Badolo A. Okado K., Aonuma H., Guelbeogo W.M., Bando H., Yoshimura A. Fukumoto S., Sagnon N'F and Kanuka H
  • Organizer: Gordon Research conference
  • Place of Presentation: Pisa, ItalyJuly
  • Year and Date: 20110731-0805