Study on canine periodontal disease as zoonosis

2013 Fiscal Year Final Research Report

• Project/Area Number: 23658256
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: Azabu University
• Principal Investigator: ASAI Fumitoshi 麻布大学, 獣医学部, 教授 (00511677)
• Co-Investigator(Kenkyū-buntansha): KATO Yukio 麻布大学, 獣医学部獣医学科, 准教授 (00224551) ; NAKANO Kazuhiko 大阪大学, 歯学研究科, 准教授 (00379083) ; NAKANO Michiyo 岡山大学, 医歯(薬)学総合研究科, 教授 (30359848)
• Project Period (FY): 2011 – 2013
• Keywords: 人獣共通感染症 / 歯周病 / 犬 / 全身疾患

Research Abstract

The objective of the present study was to determine whether dog periodontal diseases are zoonotic and clarified the relationship between periodontal and systemic diseases. Oral swab samples were collected from dogs kept as family pets. Polymerase chain reaction was used to detect the presence of periodontopathic bacterial strains—10 from humans and 1 from dogs. P. gulae was detected in nearly all of the 100 or more dogs that were analyzed. By analyzing a fimbrial gene (fimA) sequence, we identified 3 types of P. gulae: type-A, type-B, and type-C. Type-C was the most virulent. A correlation between P. gulae type-C and cardiovascular disease was found by investigating the association between periodontal and systemic diseases.

Research Products

• [Journal Article] Correlation of Age with distribution of periodontitis-related bacteria in Japanese dogs (2013)
  • Author(s): Hirai N, Shirai M, Kato Y, Murakami M, Nomura R, Yamasaki Y, Takahashi S, Kondo C, Matsumoto-Nakano M, Nakano K, Asai F
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 999-1001
  • Peer Reviewed
• [Journal Article] Diversity of fimbrillin among Porphyromonas gulae clinical isolates from Japanese dogs (2012)
  • Author(s): Nomura R, Shirai M, Kato Y, Murakami M, Nakano K, Hirai N, Mizusawa T, Naka S, Yamasaki Y, Matsumoto-Nakano M, Ooshima T, Asai F
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 885-891
  • Peer Reviewed
• [Journal Article] Distribution of periodontopathic species in dogs and their owners (2012)
  • Author(s): Yamasaki Y, Nomura R, Nakano K, Naka S, Matsumoto-Nakano M, Asai F, Ooshima T
  • Journal Title: Arch Oral Biol Volume: 57 Pages: 1183-1188
  • DOI: 10.1016/j.archoralbio.2012.02.015
  • Peer Reviewed
• [Journal Article] Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype (2012)
  • Author(s): Yamasaki Y, Nomura R, Nakano K, Inaba H, Kuboniwa M, Hirai N, Shirai M, Kato Y, Murakami M, Naka S, Iwai S, Matsumoto-Nakano M, Ooshima T, Amano A, Asai F
  • Journal Title: Vet Microbiol Volume: 161 Pages: 196-205
  • DOI: 10.1016/j.vetmic.2012.07.026
  • Peer Reviewed
• [Journal Article] Molecular Detection of the Human Periodontal Pathogens in Oral Swab Specimens from Dogs in Japan (2011)
  • Author(s): Kato Y, Shirai M, Murakami M, Mizusawa T, Hagimoto A, Wada K, Nomura R, Nakano K, Ooshima T, Asai F
  • Journal Title: J Vet Dent Volume: 28 Pages: 84-89
  • Peer Reviewed
• [Presentation] 犬とその飼い主における歯周病原性細菌の分布と伝播に関する分子生物学的検討 (2013)
  • Author(s): 山崎由衛, 仲野和彦, 野村良太, 仲 周平, 浅井史敏, 仲野道代
  • Organizer: 第34回岡山歯学会学術集会
  • Place of Presentation: 岡山 : 岡山大学
  • Year and Date: 2013-10-27
• [Presentation] 犬の歯周状態と歯肉縁下プラークに存在する歯周病原性細菌種との関連 (2012)
  • Author(s): 山崎由衛, 野村良太, 仲野和彦, 平井範彦, 倉谷礎, 白井明志, 加藤行男, 村上賢, 仲野道代, 浅井史敏
  • Organizer: 第33回 動物臨床医学会年
  • Place of Presentation: 大阪 : 大阪国際会議場
  • Year and Date: 20121117-18
• [Presentation] イヌの歯周状態と歯肉縁下プラークにおけるPorphyromonas gulae FimA 型分布との関連 (2012)
  • Author(s): 山崎由衛, 野村良太, 仲野和彦, 白井明志, 加藤行男, 村上 賢, 仲野道代, 浅井史敏
  • Organizer: 第14回 日本臨床獣医学フォーラム
  • Place of Presentation: 東京 : ホテルニューオータニ
  • Year and Date: 20120929-30
• [Presentation] イヌにおける歯周病原性細菌の保有状況一般家庭飼育犬と実験用ビーグル犬でのヒト歯周病原性細菌とPorphyromonas gluae の保有状況の比較 (2012)
  • Author(s): 平井範彦, 關恵介, 倉谷礎, 白井明志, 野村良太, 仲野和彦, 山崎由衛, 仲野道代, 浅井史敏
  • Organizer: 第14回 日本臨床獣医学フォーラム
  • Place of Presentation: 東京 : ホテルニューオータニ
  • Year and Date: 20120929-30
• [Presentation] イヌにおける歯周病原性細菌分布に関する文政生物学的検討 (2012)
  • Author(s): 山崎由衛, 野村良太, 仲野和彦, 仲周平, 仲野道代, 大島隆, 浅井史敏
  • Organizer: 第153回 日本獣医学会学術集会
  • Place of Presentation: 埼玉 : 大宮ソニックシティ
  • Year and Date: 2012-03-27
• [Presentation] イヌの口腔におけるCampyrobacter rectus の分布状況 (2011)
  • Author(s): 赤木義明, 高橋理子, 岡谷-アレシャンドレ, 村上 賢, 白井明志, 野村良太, 仲野和彦, 大島隆, 浅井史敏, 加藤行男
  • Organizer: 第152回 日本獣医学会学術集会
  • Place of Presentation: 大阪 : 大阪府立大学
  • Year and Date: 2011-09-19
• [Patent(Industrial Property Rights)] Porphyromonas gulae の線毛遺伝子型 (2012)
  • Inventor(s): 浅井史敏, 加藤行男, 白井明志, 村上賢, 仲野和彦, 野村良太
  • Industrial Property Rights Holder: 浅井史敏, 加藤行男, 白井明志, 村上賢, 仲野和彦, 野村良太
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: FA1484-12130
  • Filing Date: 2012-12-13
  • Overseas