2013 Fiscal Year Final Research Report
Study on epigenetic regulation of genes responsible for ethanol fermantation
| Project/Area Number |
23658281
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
HARATA Masahiko 東北大学, (連合)農学研究科(研究院), 准教授 (70218642)
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| Project Period (FY) |
2011 – 2012
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| Keywords | エピジェネティック / クロマチン / 転写 |
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| Research Abstract |
We deleted the budding yeast ARP6 gene and analyzed the expression of genes responsible for ethanol fermentation and spacial localization of genes in the nucleus. We also analyzed the spacial localization of a chromatin regions to which Arp6 or a histone variant H2A.Z was artificially bound. Using chromatin immunoprecipitation assay, it was shown that Arp6-bound chromatin bond to nuclear pore complex and that H2A.Z-bound chromatin bonds also to Mps3, a nuclear membrane protein. These observations suggests that these nuclear membrane proteins have roles in the spatial arrangement of genes, including ones for ethanol fermentation.
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Research Products
(13 results)
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[Journal Article] Possible involvement of LKB1-AMPK signaling in non-homologous end joining2014
Author(s)
Ui A, Ogiwara H, Nakajima S, Kanno S, Watanabe R, Harata M, Okayama H, Harris CC, Yokota J, Yasui A, Kohno T
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Journal Title
Oncogene
Volume: 33
Pages: 1640-1648
DOI
Peer Reviewed
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[Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei2012
Author(s)
Maruyama, E.O., Hori, T., Tanabe, H., Kitamura, H., Matsuda. R., Tone, S., Hozak, P., Habermann, F.A., von Hase, J., Cremer, C., Fukagawa, T., Harata, M
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Journal Title
J Cell Sci
Volume: 125
Pages: 3739-3743
DOI
Peer Reviewed
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