2012 Fiscal Year Final Research Report
Expression of secretory immunoglobulin A in lettuce suitable for the production of therapeutic antibodies in plantfactory.
| Project/Area Number |
23659067
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
IMAI Yasuyuki 静岡県立大学, 薬学部, 教授 (80160034)
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| Co-Investigator(Kenkyū-buntansha) |
KUROHANE Kohta 静岡県立大学, 薬学部, 助教 (90333525)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Hirokazu 静岡県立大学, 大学院・食品栄養環境科学研究院, 教授 (80170348)
NIWA Yasuo 静岡県立大学, 大学院・食品栄養環境科学研究院, 助教 (00222191)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 粘膜免疫 / 抗体医薬 / 免疫グロブリンA / 植物発現系 |
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| Research Abstract |
Therapeutic antibodies are widely used as molecular-targeted drugs in these days. However, they havelimitations due to the high cost and limited scalability of production. To aim at the lower cost production of antibodies, we tried to express antibody genes in lettuce, which can be cultivated in greenhousethat is useful to avoid environmental impact. We prepared cDNA of recombinant IgA specific for Shiga toxin 1 produced by Enterohaemorrhagic Escherichia coli. We constructed transgenic Arabidopsis thalianaand Lactuca sativaexpressing Stx-1-specific IgA.The expression of IgA and its toxin neutralization activity were demonstrated in vitro.
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Research Products
(16 results)
