Experimental approach for direct transformation of GABAergic neurons

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659092
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Fukui
• Principal Investigator: SATO Makoto 福井大学, 医学部, 教授 (10222019)
• Co-Investigator(Kenkyū-buntansha): KURODA Kazuki 福井大学, 医学部, 助教 (60557966) ; KOMADA Munekazu 愛知学院大学, 歯学部, 助教 (90523994)
• Project Period (FY): 2011 – 2012
• Keywords: 解剖学 / 分化 / 細胞 / 組織 / 神経科学

Research Abstract

（１） Cortical neurons that had been transfected with Ptf1a expressing vector migrated tangentially into the cortex, of which migratory behavior is typical for GABAergic neurons.
（２）Ptf1a expression did not result in transformation of glutamatergic neurons into GABAergic neurons; no specific GABAergic neuronal markers were newly expressed in glutamatergic neurons by Ptf1a expression.
（３） We collected RNAs from Ptf1a expressed neurons and searched for any Ptf1a-induced genes, then we identified several candidate genes.

Research Products

• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration (2013)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M
  • Journal Title: Cerebral Cortex Volume: 23(6) Pages: 1410-1423
  • Peer Reviewed
• [Journal Article] Post-natal treatment by a blood-brain-barrier permeable calpain inhibitor, SNJ1945 rescued defective neuronal migration and neuronal circuit formation in lissencephaly (2013)
  • Author(s): Toba, S., Tamura, Y., Kumamoto, K., Yamada, M., Takao, K., Hattori, S., Miyakawa, T., Kataoka, Y., Azuma, M.,Hayasaka, K., Amamoto, M., Tominaga, K., Wynshaw-Boris, A., Wanibuchi, H., Oka, Y., Sato, M., Kato, M. and Hirotsune, S.
  • Journal Title: Sci. Rep Volume: 3 Pages: 1224
  • Peer Reviewed
• [Journal Article] Long-term oxytocin administration improves social behaviors in a girl with autistic disorder. (case report) (2012)
  • Author(s): Kosaka, H., Munesue, T., Ishitobi, M., Asano, M., Omori, M., Sato, M., Tomoda, A. and Wada, Y
  • Journal Title: BMC Psychiatry Volume: 12(1) Pages: 110
  • Peer Reviewed
• [Journal Article] Activation of Aurora-A is essential for neuronal migration via modulation of microtubule organization (2012)
  • Author(s): Takitoh, T., Kumamoto, K., Wang C.-C., Sato, M., Toba, S., Wynshaw-Boris, A. and Hirotsune, S
  • Journal Title: J. Neurosci Volume: 32(32) Pages: 11050-11066
  • Peer Reviewed
• [Journal Article] Effects of antidepressants and mood stabilizers on serum levels of adiponectin (2012)
  • Author(s): Omata, N., Murata, T., Narita, K., Maruoka, N., Mitsuya, H., Mita, K., Nishimoto, T., Sato, M. and Wada, Y
  • Journal Title: NeuroEndocrinol. Lett. Volume: 33(1) Pages: 1-2
  • Peer Reviewed
• [Journal Article] Maternal bisphenol A oral dosing relates to the acceleration of neurogenesis in the developing neocortex of mouse fetuses (2012)
  • Author(s): Komada, M., Asai, Y ., Morii, M., Matsuki, M., Sato, M. and Nagao, T
  • Journal Title: Toxicology. Volume: 16;295(1-3) Pages: 31-8
  • Peer Reviewed
• [Journal Article] A tightly controlled conditional knockdown system using the tol2 transposon-mediated technique (2012)
  • Author(s): Iguchi, T., Yagi, H., Wang, CC. and Sato, M
  • Journal Title: PLoS ONE Volume: 7(3) Pages: e33380
  • Peer Reviewed
• [Journal Article] GPR98/Gpr98 Gene Is Involved in the Regulation of Human and Mouse Bone Mineral Density (2012)
  • Author(s): Urano, T., Shiraki, M,. Yagi, H., Ito, M., Sasaki, N., Sato, M., Ouchi, Y . and Inoue, S
  • Journal Title: J. Clin. Endocrinol. Metab Volume: 97(4) Pages: E565-74
  • Peer Reviewed
• [Journal Article] iPS 細胞研究とその周辺技術 (2011)
  • Author(s): 黒田一樹, 佐藤 真
  • Journal Title: 特集 1 神経系における iPS 細胞(iPS 細胞の活用も含めた神経機能修復の現状と将来):脳 21 Volume: Vol.14 No.3 Pages: 30(234)-34(238)
• [Presentation] マウス大脳皮質長連合ニューロン軸索の可視化の試み (2013)
  • Author(s): 岡 雄一郎,猪口徳一,佐藤 真
  • Organizer: ポスター(3P-G011)第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-30
• [Presentation] 神経細胞の棘突起(スパイン)形成における FILIP 関連分子の機能解析 (2013)
  • Author(s): 黒田一樹,謝 敏カク,猪口徳一,岡 雄一郎,尾身 実,王 振吉,八木秀司,佐藤 真
  • Organizer: ポスター(3P-G020)第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-30
• [Presentation] 非筋肉型ミオシン分子の細胞内局在を調節する新たな機構.ポスター(1P-G054) (2013)
  • Author(s): 八木秀司,野口光一,佐藤 真
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-28
• [Presentation] Filip is involved in the morphological control of dendritic spine through actomyosin regulation (2012)
  • Author(s): Yagi, H., Kuroda, K., Xie, M.-J. Ikeda, H., Iguchi, T., Komada, M., Murase K., Okabe M., Noguchi, K. and Sato, M
  • Organizer: ポスター(636.11/B30) Society For Neuroscience 2012
  • Place of Presentation: ニューオリンズ(U.S.A)
  • Year and Date: 2012-10-16
• [Presentation] A2-pancortins especially Pancortin-4, are involved in the neuronal cell desth after the ischemic stroke during the growth period (2012)
  • Author(s): Wang, C.-C., Kuroda, K., Xie, M.-J., Oka, Y., Iguchi, T., Matsuyama, T., and Sato, M
  • Organizer: ポ ス タ ー (P02-1) The 11thBiennial Meeting of the Asian-Pacific Society for Neurochemistry / 55thAnnual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-30
• [Presentation] Analyses on the potential candidates of collateral branch inducing factor that are specifically expressed in the targets of axon collaterals from the corticospinal projection. (2012)
  • Author(s): Iguchi, T., Oka, Y., Kuroda, K., Wang, C.-C., Xie, M.-J., Yagi, H., and Sato, M
  • Organizer: ポスター(P01-14)The 11thBiennial Meeting of the Asian-Pacific Society for Neurochemistry / 55thAnnual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-29
• [Presentation] FILIP-related molecule controls spine maturation in the hippcampal neuron via non-muscle myosin llb (2012)
  • Author(s): Kuroda, K., Yagi, H., Xie, M.-J., Oka, Y., Iguchi, T., and Sato, M
  • Organizer: ポスター(P01-36)The 11thBiennial Meeting of the Asian-Pacific Society for Neurochemistry / 55thAnnual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-29
• [Presentation] WAVE-Abi2 複合体は radial migration 時の成長円錐の活性化およびmultipolar-bipolar transition と glia-guided migration の開始を制御する (2012)
  • Author(s): 謝 敏カク,八木秀司, 黒田一樹,王 振吉,駒田致和,岡 雄一郎,猪口徳一,佐藤 真
  • Organizer: ポスター(P4-d05)第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-21
• [Presentation] マウス大脳皮質長連合ニューロン特異的遺伝子の同定 (2012)
  • Author(s): 岡 雄一郎,猪口徳一,佐藤 真
  • Organizer: ポスター(P2-c13)第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] FILIP によるアクチンミオシン調節能の棘突起動態変化への関与 (2012)
  • Author(s): 八木秀司,黒田一樹,謝 敏カク,永野隆,池田 弘,猪口徳一,村瀬一之,岡部 勝,佐藤 真
  • Organizer: ポスター(P2-f22)第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] 大脳皮質形成とスパイン形成の新たな共通分子基盤とその展開 (2012)
  • Author(s): 佐藤 真
  • Organizer: シンポジウム(S19-3)「神経発生・形成における細胞骨格の意義」第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling (2011)
  • Author(s): 佐藤 真
  • Organizer: AACL -2011 (Asian Aging Core for Longevity) 長崎シンポジウム Japan-Korea Joint Conference on Brain Aging and Neurodegeneration
  • Place of Presentation: 長崎
  • Year and Date: 2011-11-21
• [Presentation] Dendritic growth cone activities, regulated by Abl kinase and Cdk5 via WAVE2-Abi2, are essential for completing multipolar-bipolar transition and starting glia-guided locomotion (2011)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Iguchi, T. and Sato, M.
  • Organizer: ポスター(A4 540.04)Society For Neuroscience 201
  • Place of Presentation: ワシントン (U.S.A)
  • Year and Date: 2011-11-15
• [Presentation] dendritic growth cone activity regulated by Abl kinase and Cdk5 via WAVE2-Abi2 is essential for completing the multipolar-bipolar transition and initiating glia-guided locomotion (2011)
  • Author(s): 佐藤 真
  • Organizer: シンポジウム(S06-2)「神経形態制御から機能へ -スペーシャルセルバイオロジーの観点から」第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
  • Year and Date: 2011-09-27
• [Remarks]
  • URL: http://www.med.u-fukui.ac.jp/KAIBOU2/