Identification and characterization of desacyl-ghrelin

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659124
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Kagoshima University
• Principal Investigator: UEDA Masahiro 鹿児島大学, 自然科学教育研究支援センター, 技術職員 (00448573)
• Co-Investigator(Kenkyū-buntansha): KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064) ; IIJIMA Mikio 鹿児島大学, 医歯学総合研究科, 助教 (00305111) ; KISHIDA Michiko 鹿児島大学, 医歯学総合研究科, 助教 (40274089)
• Co-Investigator(Renkei-kenkyūsha): INUI Akio 鹿児島大学, 医歯学総合研究科, 教授 (80168418)
• Project Period (FY): 2011 – 2012
• Keywords: 摂食調節

Research Abstract

Ghrelin is a peptide hormone to regulate appetite and is known to have two forms (acyl ghrelin and des-acyl ghrelin). Ghrelin receptor (GHSR), which recognizes the post-translational acyl-modification, expressed in various tissues. However, ghrelin without lipid-modification does not bind to GHSR and functions antagonistic to acyl ghrelin. These findings suggested the presenceof unknown receptor specific for des-acyl ghrelin. We performed affinity-purification of des-acyl ghrelin-binding protein using pig brain extract. We tried to elute des-acyl ghrelin-associated protein by high salt condition (1M NaCl) but detected no significant condensation of candidate molecules. Next, we performed conventional receptor panning assay using COS7 cells transfected with sub-pooled mouse brain cDNA libraries. We detected several pools as “positive pools” and further selection of procedures for cloning is in process.

Research Products

• [Journal Article] Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells (2012)
  • Author(s): Takehiro Hayashi, Michiko Kishida, Yoshiaki Nishizawa, Mikio Iijima, Chihaya Koriyama, Masayuki Nakamura, Akio Sano, Shosei Kishida
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 419 Pages: 511-516
  • DOI: DOI:10.1016/j.bbrc.2012.02.047
• [Journal Article] The clinical use of Kampo medicines (traditional Japanese herbal treatments) for controlling cancer patients' symptoms in Japan: a national cross-sectional survey (2012)
  • Author(s): Iwase S, Yamaguchi T, Miyaji T, Terawaki K, Inui A, Uezono Y
  • Journal Title: BMC Complement Altern Med Volume: 12 Pages: 222
  • DOI: DOI:10.1186/1472-6882-12-222
• [Journal Article] ntelligence quotient and cognitive functions in severerestricting-type anorexia nervosa before and after weight gain. (2012)
  • Author(s): Koyama KI, Asakawa A, Nakahara T, Amitani H, Amitani M, Saito M, Taruno Y, Zoshiki T, Cheng KC, Yasuhara D, Inui A
  • Journal Title: Nutrition. Volume: 28(11-12) Pages: 1132-6
  • DOI: DOI:10.1016/j.nut.2012.03.003
• [Journal Article] Effects of supplementation on food intake, body weight and hepatic metabolites in the citrin/mitochondrial glycerol-3-phosphate dehydrogenase double-knockout mouse model of human citrin deficiency (2012)
  • Author(s): Saheki T, Inoue K, Ono H, Katsura N, Yokogawa M, Yoshidumi Y, Furuie S, Kuroda E, Ushikai M, Asakawa A, Inui A, Eto K, Kadowaki T, Sinasac DS, Yamamura K, Kobayashi K
  • Journal Title: Mol Genet Metab Volume: 107(3) Pages: 322-9
  • DOI: DOI:10.1016/j.ymgme.2012.07.021
• [Journal Article] Alteration of antral and proximal colonic motility induced by chronic psychological stress involves central urocortin 3 and vasopressin in rats (2012)
  • Author(s): Ataka K, Nagaishi K, Asakawa A, Inui A, Fujimiya M
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 303(4) Pages: G519-28
  • DOI: DOI:10.1152/ajpgi.00390.2011
• [Journal Article] Plasma ghrelin isoforms and gastric ghrelin O-acyltransferase expression are influenced by Helicobacter pylori status (2012)
  • Author(s): Ando T, Mizuno S, Ishida T, Kondo Y, Miki I, Yoshida M, Azuma T, Ishikawa T, Takagi T, Yagi N, Kokura S, Naito Y, Yoshikawa T, Asakawa A, Inui A
  • Journal Title: Nutrition. Volume: 28(10) Pages: 967-72
  • DOI: DOI:10.1016/j.nut.2011.11.023
• [Journal Article] A case of anorexia nervosa with disseminated intravascular coagulation syndrome (2012)
  • Author(s): Haruta I, Asakawa A, Ogiso K, Amitani H, Amitani M, Tsai M, Hamada S, Inui A
  • Journal Title: Int J Eat Disord Volume: 45(3) Pages: 453-5
  • DOI: DOI:10.1002/eat.20954
• [Journal Article] Kobe earthquake and post-traumatic stress in school-aged children (2012)
  • Author(s): Uemoto M, Asakawa A, Takamiya S, Asakawa K, Inui A
  • Journal Title: Int J Behav Med Volume: 19(3) Pages: 243-51
  • DOI: DOI:10.1007/s12529-011-9184-3
• [Journal Article] Characterization of preptin-induced insulin secretion in pancreatic β-cells (2012)
  • Author(s): Cheng KC, Li YX, Asakawa A, Ushikai M, Kato I, Sato Y, Cheng JT, Inui A
  • Journal Title: J Endocrinol Volume: 215(1) Pages: 43-9
  • DOI: DOI:10.1530/JOE-12-0176
• [Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes (2011)
  • Author(s): Toshiro Kibe, Michiko Kishida, Masayuki Kamino, Mikio Iijima, Lin Chen, Mika Habu, Akihiko Miyawaki, Hiroshi Hijioka, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
  • Journal Title: Oral Science International Volume: 8 Pages: 20-28
  • DOI: DOI:10.1016/S1348-8643(11)00009-7
• [Journal Article] A novel ameloblastoma cell line(AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis、Oral Surg
  • Author(s): Toshiro Kibe, Takao Fuchigami, Michiko Kishida, Mikio Iijima, Kiyohide Ishihata, Hiroshi Hijioka, Akihiko Miyawaki, Ichiro Semba, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
  • Journal Title: Oral Med.Oral Pathol.Oral Radiol Volume: (印刷中)
  • DOI: DOI:10.1016/j.oooo.2013.03.005
• [Presentation] Herbal (Kampo) Medicine and Anorexia-Cachexia Syndrome (2012)
  • Author(s): Akio Inui
  • Organizer: Neuroscience Seminar Series Garvan Institute of Medical Research
  • Place of Presentation: Sydney
  • Year and Date: 2012-12-12
• [Presentation] Ghrelin and Energy Homeostasis Focused on Cancer Anorexia-Cachexia Syndrome (2012)
  • Author(s): Akio Inui
  • Organizer: The 9thInternational Symposium on Growth and Nutrition in Children with Chronic Kidney Diseases
  • Place of Presentation: San Diego
  • Year and Date: 2012-04-13
• [Presentation] Etude de l'expression de la ghreline central,l'anxieteet la depression dans un modele d'anorexie induite par le methotrexate chezle rat (2012)
  • Author(s): Francois M, Takagi K, Tennoune N, Beutheu Youmba S, Bole-Feysot C, Cravezic A, do Rego JC, Coeffier M, Inui A, Dechelotte P, Fetissov SO
  • Organizer: 1er Colloque du GIR-AFDAS-TCA(groupe interdisciplinaire de recherche de l'association francaise pour le developpement des approaches specialisees des troubles du comportement alimentaire)
  • Place of Presentation: Paris
  • Year and Date: 2012-02-09