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2012 Fiscal Year Final Research Report

Identification and characterization of desacyl-ghrelin

Research Project

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Project/Area Number 23659124
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKagoshima University

Principal Investigator

UEDA Masahiro  鹿児島大学, 自然科学教育研究支援センター, 技術職員 (00448573)

Co-Investigator(Kenkyū-buntansha) KISHIDA Shosei  鹿児島大学, 医歯学総合研究科, 教授 (50274064)
IIJIMA Mikio  鹿児島大学, 医歯学総合研究科, 助教 (00305111)
KISHIDA Michiko  鹿児島大学, 医歯学総合研究科, 助教 (40274089)
Co-Investigator(Renkei-kenkyūsha) INUI Akio  鹿児島大学, 医歯学総合研究科, 教授 (80168418)
Project Period (FY) 2011 – 2012
Keywords摂食調節
Research Abstract

Ghrelin is a peptide hormone to regulate appetite and is known to have two forms (acyl ghrelin and des-acyl ghrelin). Ghrelin receptor (GHSR), which recognizes the post-translational acyl-modification, expressed in various tissues. However, ghrelin without lipid-modification does not bind to GHSR and functions antagonistic to acyl ghrelin. These findings suggested the presenceof unknown receptor specific for des-acyl ghrelin. We performed affinity-purification of des-acyl ghrelin-binding protein using pig brain extract. We tried to elute des-acyl ghrelin-associated protein by high salt condition (1M NaCl) but detected no significant condensation of candidate molecules. Next, we performed conventional receptor panning assay using COS7 cells transfected with sub-pooled mouse brain cDNA libraries. We detected several pools as “positive pools” and further selection of procedures for cloning is in process.

  • Research Products

    (14 results)

All 2012 2011 Other

All Journal Article (11 results) Presentation (3 results)

  • [Journal Article] Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells2012

    • Author(s)
      Takehiro Hayashi, Michiko Kishida, Yoshiaki Nishizawa, Mikio Iijima, Chihaya Koriyama, Masayuki Nakamura, Akio Sano, Shosei Kishida
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 419 Pages: 511-516

    • DOI

      DOI:10.1016/j.bbrc.2012.02.047

  • [Journal Article] The clinical use of Kampo medicines (traditional Japanese herbal treatments) for controlling cancer patients' symptoms in Japan: a national cross-sectional survey2012

    • Author(s)
      Iwase S, Yamaguchi T, Miyaji T, Terawaki K, Inui A, Uezono Y
    • Journal Title

      BMC Complement Altern Med

      Volume: 12 Pages: 222

    • DOI

      DOI:10.1186/1472-6882-12-222

  • [Journal Article] ntelligence quotient and cognitive functions in severerestricting-type anorexia nervosa before and after weight gain.2012

    • Author(s)
      Koyama KI, Asakawa A, Nakahara T, Amitani H, Amitani M, Saito M, Taruno Y, Zoshiki T, Cheng KC, Yasuhara D, Inui A
    • Journal Title

      Nutrition.

      Volume: 28(11-12) Pages: 1132-6

    • DOI

      DOI:10.1016/j.nut.2012.03.003

  • [Journal Article] Effects of supplementation on food intake, body weight and hepatic metabolites in the citrin/mitochondrial glycerol-3-phosphate dehydrogenase double-knockout mouse model of human citrin deficiency2012

    • Author(s)
      Saheki T, Inoue K, Ono H, Katsura N, Yokogawa M, Yoshidumi Y, Furuie S, Kuroda E, Ushikai M, Asakawa A, Inui A, Eto K, Kadowaki T, Sinasac DS, Yamamura K, Kobayashi K
    • Journal Title

      Mol Genet Metab

      Volume: 107(3) Pages: 322-9

    • DOI

      DOI:10.1016/j.ymgme.2012.07.021

  • [Journal Article] Alteration of antral and proximal colonic motility induced by chronic psychological stress involves central urocortin 3 and vasopressin in rats2012

    • Author(s)
      Ataka K, Nagaishi K, Asakawa A, Inui A, Fujimiya M
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol

      Volume: 303(4) Pages: G519-28

    • DOI

      DOI:10.1152/ajpgi.00390.2011

  • [Journal Article] Plasma ghrelin isoforms and gastric ghrelin O-acyltransferase expression are influenced by Helicobacter pylori status2012

    • Author(s)
      Ando T, Mizuno S, Ishida T, Kondo Y, Miki I, Yoshida M, Azuma T, Ishikawa T, Takagi T, Yagi N, Kokura S, Naito Y, Yoshikawa T, Asakawa A, Inui A
    • Journal Title

      Nutrition.

      Volume: 28(10) Pages: 967-72

    • DOI

      DOI:10.1016/j.nut.2011.11.023

  • [Journal Article] A case of anorexia nervosa with disseminated intravascular coagulation syndrome2012

    • Author(s)
      Haruta I, Asakawa A, Ogiso K, Amitani H, Amitani M, Tsai M, Hamada S, Inui A
    • Journal Title

      Int J Eat Disord

      Volume: 45(3) Pages: 453-5

    • DOI

      DOI:10.1002/eat.20954

  • [Journal Article] Kobe earthquake and post-traumatic stress in school-aged children2012

    • Author(s)
      Uemoto M, Asakawa A, Takamiya S, Asakawa K, Inui A
    • Journal Title

      Int J Behav Med

      Volume: 19(3) Pages: 243-51

    • DOI

      DOI:10.1007/s12529-011-9184-3

  • [Journal Article] Characterization of preptin-induced insulin secretion in pancreatic β-cells2012

    • Author(s)
      Cheng KC, Li YX, Asakawa A, Ushikai M, Kato I, Sato Y, Cheng JT, Inui A
    • Journal Title

      J Endocrinol

      Volume: 215(1) Pages: 43-9

    • DOI

      DOI:10.1530/JOE-12-0176

  • [Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes2011

    • Author(s)
      Toshiro Kibe, Michiko Kishida, Masayuki Kamino, Mikio Iijima, Lin Chen, Mika Habu, Akihiko Miyawaki, Hiroshi Hijioka, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
    • Journal Title

      Oral Science International

      Volume: 8 Pages: 20-28

    • DOI

      DOI:10.1016/S1348-8643(11)00009-7

  • [Journal Article] A novel ameloblastoma cell line(AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis、Oral Surg

    • Author(s)
      Toshiro Kibe, Takao Fuchigami, Michiko Kishida, Mikio Iijima, Kiyohide Ishihata, Hiroshi Hijioka, Akihiko Miyawaki, Ichiro Semba, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
    • Journal Title

      Oral Med.Oral Pathol.Oral Radiol

      Volume: (印刷中)

    • DOI

      DOI:10.1016/j.oooo.2013.03.005

  • [Presentation] Herbal (Kampo) Medicine and Anorexia-Cachexia Syndrome2012

    • Author(s)
      Akio Inui
    • Organizer
      Neuroscience Seminar Series Garvan Institute of Medical Research
    • Place of Presentation
      Sydney
    • Year and Date
      2012-12-12
  • [Presentation] Ghrelin and Energy Homeostasis Focused on Cancer Anorexia-Cachexia Syndrome2012

    • Author(s)
      Akio Inui
    • Organizer
      The 9thInternational Symposium on Growth and Nutrition in Children with Chronic Kidney Diseases
    • Place of Presentation
      San Diego
    • Year and Date
      2012-04-13
  • [Presentation] Etude de l'expression de la ghreline central,l'anxieteet la depression dans un modele d'anorexie induite par le methotrexate chezle rat2012

    • Author(s)
      Francois M, Takagi K, Tennoune N, Beutheu Youmba S, Bole-Feysot C, Cravezic A, do Rego JC, Coeffier M, Inui A, Dechelotte P, Fetissov SO
    • Organizer
      1er Colloque du GIR-AFDAS-TCA(groupe interdisciplinaire de recherche de l'association francaise pour le developpement des approaches specialisees des troubles du comportement alimentaire)
    • Place of Presentation
      Paris
    • Year and Date
      2012-02-09

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Published: 2014-09-25   Modified: 2015-11-26  

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