2012 Fiscal Year Final Research Report
Identification of GPCR binding proteins by using a novel anti-FLAG antibody and magnetic nanobeads
| Project/Area Number |
23659157
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Juntendo University (2012)
Kyushu University (2011) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | GPCR / 免疫沈降 / 結合タンパク質 |
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| Research Abstract |
I established a novel anti-FLAG monoclonal antibody (2H8) with high-affinify and a low background. Combination of 2H8 antibody with a magnetic nanobeads enabled us to immunoprecipitate FLAG-tagged G-protein coupled receptors (GPCRs). Immunoprecipitated GPCRs were detectable by silver staining after separation by SDS-Page. I am analyzing some putative GPCR-binding proteins isolated by this novel immunoprecipitation techniques.
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