2013 Fiscal Year Final Research Report
Development of a new in situ technique demonstrating methyaled cytosine by iso-thermal DNA amplification method
| Project/Area Number |
23659186
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Ehime University |
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Principal Investigator |
KITAZAWA Sohei 愛媛大学, 医学(系)研究科(研究院), 教授 (90186239)
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| Co-Investigator(Kenkyū-buntansha) |
KITAZAWA Riko 愛媛大学, 大学院・医学系研究科, 准教授 (00273780)
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| Project Period (FY) |
2011 – 2013
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| Keywords | DNA / メチル化シトシン / 組織細胞化学 / 遺伝子増幅 / in situ hybridization |
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| Research Abstract |
By making a ICON probe which specifically bound to methylated cytosine on chromosomal DNA in a sequence specific manner, a strong chemical cross-linking was made by osmium reaction. By so-called PRINS method, a DNA elongation reaction was established using bounded ICON probe on the specific chromosome. Modified nucleotides were incorporated during this elongation stem and visualized by a conventional histochemical techniques. By this easy, methyalation status of specific chromosome or DNA sequence could be visualized. So far we gathered a lot of basic and fundamental data. We are now currently trying to visualize a single-copy gene during spermatogenesis in mouse testis, and updating our protocol for more stable and reliable one.
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[Journal Article] Nonalcoholic fatty liver disease in adult hypopituitary patients with growth hormone deficiency and the impact of growth hormone replacement therapy2012
Author(s)
Hitoshi Nishizawa, Genzo Iguchi, Ayumi Murawaki, Hidenori Fukuoka, Yoshitake Hayashi, Hidesuke Kaji, Masaaki Yamamoto, Kentaro Suda, Michiko Takahashi, Yasushi Seo, Yoshihiko Yano, Riko Kitazawa, Sohei Kitazawa, Masafumi Koga, Yasuhiko Okimura, Kazuo Chihara, Yutaka Takahashi
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Journal Title
Eur J Endcri
Volume: 167(1)
Pages: 67-74
DOI
Peer Reviewed
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[Journal Article] Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting osteoclastogenesis2012
Author(s)
Wang Y, Liu1 W, Masuyama R, Fukuyama R, Ito M, Zhang Q, Komori H, Murakami T, Moriishi T, Miyazaki T, Kitazawa R, Yoshida CA, Kawai Y, Izumi S, Komori T
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Journal Title
Bone
Volume: 50(1)
Pages: 09-419
DOI
Peer Reviewed
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[Journal Article] PGRN is a Key Adipokine Mediating High Fat Diet-Induced Insulin Resistance and Obesity through IL-6 in Adipose Tissue2012
Author(s)
Toshiya Matsubara, Ayako Mita, Kohtaro Minami, Tetsuya Hosooka, Sohei Kitazawa, Kenichi Takahashi, Yoshikazu Tamori, Norihide Yokoi, Makoto Watanabe, Ei-ichi Matsuo, Osamu Nishimura, Susumu Seino
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Journal Title
Cell Metabolism
Volume: 15(1)
Pages: 38-50
DOI
Peer Reviewed
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[Journal Article] Parathyroid hormone-responsive Smad3- related factor, Tmem119, promotes osteoblast differentiation and interacts with the bone morphogenetic protein-Runx2 pathway2011
Author(s)
Itoko Hisa, Yoshifumi Inoue, Geoffrey N. Hendy, Lucie Canaff, Riko Kitazawa, Sohei Kitazawa, Toshihisa Komori, Toshitsugu Sugimoto, Susumu Seino, and Hiroshi Kaji
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Journal Title
J Biol Chem
Volume: 286(11)
Pages: 9787-9796
DOI
Peer Reviewed
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[Journal Article] Carvedilol ameliorates low-turnover bone disease in non-obese type 2 diabetes2011
Author(s)
Shunsuke Goto, Hideki Fujii, Keiji Kono, Kentaro Nakai, Yasuhiro Hamada, Hideyuki Yamato, Masami Shinohara, Riko Kitazawa, Sohei Kitazawa, Shinichi Nishi, and Masafumi Fukagaw
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Journal Title
Am J Nephrol
Volume: 34(3)
Pages: 281-290
DOI
Peer Reviewed
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[Journal Article] Chemerin regulates β-cell function in mice2011
Author(s)
Michiko Takahashi, Yasuhiko Okimura, Genzo Iguchi, Hitoshi Nishizawa, Masaaki Yamamoto, Kentaro Suda, Riko Kitazawa, Wakako Fujimoto, Kenichi Takahashi, Fyodor N. Zolotaryov, Kyoung Su Hong, Hiroshi Kiyonari, Takaya Abe, Hidesuke Kaji, Sohei Kitazawa, Masato Kasuga, Kazuo Chihara & Yutaka Takahashi
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Journal Title
Scientific
Volume: 1
Pages: 123
DOI
Peer Reviewed
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[Journal Article] Vitamin D Activates the Nrf2-Keap1 Antioxidant Pathway and Ameliorates Nephropathy in Diabetic Rats
Author(s)
Kentaro Nakai, Hideki Fujii, Keiji Kono, Shunsuke Goto, Riko Kitazawa, Sohei Kitazawa, Michinori Hirata, Masami Shinohara, Masafumi Fukagawa, and Shinichi Nishi
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Journal Title
American Journal of Hypertension, advanced online
DOI
Peer Reviewed
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