2012 Fiscal Year Final Research Report
Novel therapy of myxoid liposarcoma by Chromosome re-programming
| Project/Area Number |
23659192
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脂肪肉腫 / 染色体転座 / TLS-CHOP 遺伝子 / iPS 細胞 |
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| Research Abstract |
Myxoid liposarcomas (MLSs) are characterized by t(12;16)(q13;p11) translocation and expression of TLS-CHOP chimeric oncoprotein. In this study, we have found that miR-486 regulate differentiation of adipocyte. Furthermore, miR-486 expression was repressed in TLS-CHOP-expressed NIH3T3 fibroblasts and MLS tissues, and exogenous overexpression of miR-486 repressed growth of MLS cells. In addition, we have identified plasminogen activator inhibitor-1 (PAI-1) as a novel target gene of miR-486. Furthermore, knockdown of PAI-1 by a specific small interfering RNA (siRNA) inhibited growth of MLS cells, suggesting that increased expression of PAI-1 by miR-486 repression is critical for survival of MLS cells. Collectively, these results suggest a novel essential molecular mechanism that TLS-CHOP activates PAI-1 expression by repression of miR-486 expression in MLS tumorigenesis and development.
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[Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells2013
Author(s)
Yoon JH, Sudo K, Han JS, Chae MY, Park SH, Weinstein M, Nakae S, Yamashita T, Lee IK, Sumida T, Kuroda M, and Mamura M
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Journal Title
European Journal of Immunology -2
Volume: Manuscript ID: eji.201242972.R1
Peer Reviewed
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[Journal Article] GKR6-deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance2013
Author(s)
Nakaya M, Tajima M, Kosako H, Nakaya T, Hashimoto A, Watari K, Nishihara H, Ohba M, Komiya S, Tani N, Nishida M, Taniguchi H, Sato Y, Matsumoto M, Tsuda M, Kuroda M, Inoue K, and Kurose H
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Journal Title
Nature Communications
Volume: 4
Pages: 1532
DOI
Peer Reviewed
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[Journal Article] The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis2013
Author(s)
Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, MaedaA, Kumada T, Kawada N, Kubo S and Kuroda M
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Journal Title
BMC Cancer (Section: Genetics, genomics and epigenetics)
Volume: 13
Pages: 99
DOI
Peer Reviewed
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[Journal Article] Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells2013
Author(s)
Ohno S, Takanashi M, Sudo K, UedaS, Ishikawa A, Matsuyama N, FujitaK, Mizutani T, Ohgi T, Ochiya T, Gotoh N, Kuroda M
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Journal Title
Molecular Therapy
Volume: vol.21 no.1
Pages: 185-191
DOI
Peer Reviewed
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[Journal Article] LGR5-Positive Colon Cancer Ctem Cells Interconvert with Drug-Resistant LGR5-Negative Cells and Are Capable of Tumor Reconstitution.2012
Author(s)
Kobayashi S, Yamada-Okabe H, Suzuki M, Natori O, Kato A, Matsubara K, Chen YJ, Yamazaki M, Funahashi S, Yoshida K, HashimotoE, Watanabe Y, Mutoh H, AshiharaM, Kato C, Watanabe T, YoshikuboT, Tamaoki N, Ochiya T, Kuroda M,Levine AJ, Yamazaki
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Journal Title
STEM CELLS.
Volume: vol 30, No.12
Pages: 2631-2644
DOI
Peer Reviewed
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[Journal Article] TLS-CHOP represses miR-486 expression, inducing upregulation of a metastasis regulator PAI-1 in human myxoid liposarcoma2012
Author(s)
Borjigin N, Ohno S, Wu W, TanakaM, Suzuki R, Fujita K, TakanashiM, Oikawa K, Goto T, Motoi T, Kosaka T, Yamamoto K, Kuroda M
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 427(2)
Pages: 355-60
DOI
Peer Reviewed
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[Journal Article] Drug-resistant colon cancer stem cells are Lgr5-negative and are capable of tumor reconstitution progressing through a Lgr5-positive state2012
Author(s)
Kobayashi S, Yamada-Okabe H, Suzuki M, Natori O, Kato A,Matsubara K, Chen YJ, Yamazaki M, Funahashi S, Yoshida K, HashimotoE, Watanabe Y, Mutoh H, Ashihara M, Kato C, Watanabe T, YoshikuboT, Tamaoki N, Ochiya T, Kuroda M, Levine AJ, and Yama
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Journal Title
Stem Cells
Volume: 30(12)
Pages: 2631-44
DOI
Peer Reviewed
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[Presentation] KLF5 is essential for oncogenesis of intestinal tumors and control of intestinal stem cells2012
Author(s)
Nakaya T, Ogawa S, Manabe I, TanakaM, Sanada M, Sato T, Goto M, Taketo M,Nakao K, Fukayama M, Kuroda M, Nagai R
Organizer
第35回分子生物学会年会
Place of Presentation
福岡
Year and Date
2012-12-11
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[Presentation] Regulation of the development of acute hepatitis by IL-23 through IL-22 and IL-17 production2011
Author(s)
Xu M, Morishima N Mizoguchi I, Chiba Y, Fujita K, Kuroda M, Iwakura Y, Cua DJ, Yasutomo K, Mizuguchi J, Yoshimoto T
Organizer
IMMUNOLOGY 2011, 98th Annual Meeting of The American Association of Immunologists
Place of Presentation
San Francisco, California
Year and Date
2011-05-13
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[Presentation] The signification of lactoferrin in secondary corneal amyloidosis.2011
Author(s)
Hirano K, Araki-Sasaki K, OsakabeY, Tanaka K, Kuroda M, Fukuoka H, Kawasaki S, Maeda N, Kinoshita S
Organizer
2011 ARVO Annual Meeting
Place of Presentation
Fort Lauderdale, Florida, USA
Year and Date
2011-05-01
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