Application of novel antibodies in pathology

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659195
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: KASAHARA Masanori 北海道大学, 大学院・医学研究科, 教授 (30241318)
• Project Period (FY): 2011 – 2012
• Keywords: 新規抗体

Research Abstract

Recently, cyclostomes were found to possess variable lymphocyte receptors (VLR) with specificity comparable to that of immunoglobulins (Ig). Until recently, only two types of VLR, VLRA and VLRB, had been identified. We identified a third VLR and named it VLRC. Our present work showed that, unlike VLRB but similar to VLRA, VLRC does not have a secretory form. Thus, among the three VLRs, VLRB appears most suitable for use as substitutes for Ig.

Research Products

• [Journal Article] Thymoproteasome: roles in thymic selection and clinical applications as a diagnostic marker of thymic epithelial tumors (2013)
  • Author(s): Tomaru, U. and Kasahara, M
  • Journal Title: Arch. Immunol. Ther. Exp. Volume: 62(in press)
  • Peer Reviewed
• [Journal Article] Protective roles of epithelial cells in the survival of adult T-cell leukemia/lymphoma cells (2013)
  • Author(s): Miyatake, Y., Oliveira, A. L. A., Jarboui, M.A., Ota, S., Tomaru, U., Teshima, T., Hall, W. W. and Kasahara, M
  • Journal Title: Am. J. Pathol Volume: 182 Pages: 1832-1842
  • Peer Reviewed
• [Journal Article] Impact of whole-genome duplication on vertebrate development and evolution (2013)
  • Author(s): Kasahara, M
  • Journal Title: Semin. Cell Dev. Biol Volume: 24 Pages: 81-82
  • Peer Reviewed
• [Journal Article] Overexpression of TNF-α- converting enzyme in fibroblasts augments dermal fibrosis after inflammation (2013)
  • Author(s): Fukaya, S., Matsui, Y., Tomaru, U., Kawakami, A., Sogo, S., Bohgaki, T., Atsumi, T., Koike, T., Kasahara, M. and Ishizu, A
  • Journal Title: Lab.Invest. Volume: 93 Pages: 72-80
  • Peer Reviewed
• [Journal Article] Involvement of human anti-mouse antibodies in false-positive results in flow cytometry due to Fc receptor-mediated trogocytosis (2012)
  • Author(s): Masuda, S., Iwasaki, S., Tomaru, U., Sato, J., Kawakami, A., Ichijo, K., Sogo, S., Baba, T., Katsumata, K., Kasahara, M. and Ishizu, A
  • Journal Title: PLoS ONE Volume: 7(12) Pages: e52918
  • DOI: Doi:10.1371/journal.pone.0052918
  • Peer Reviewed
• [Journal Article] Decrease of peripheral and intestinal NKG2A-positive T cells inpatients with ulcerative colitis (2012)
  • Author(s): Katsurada, T., Kobayashi, W., Tomaru, U., Baba, T., Furukawa, S., Ishizu, A., Takeda, K., Sakamoto, N., Asaka, M., Takeda, H. and Kasahara, M
  • Journal Title: PLoS ONE7 Pages: e44113
  • DOI: Doi:10.1371/journal.pone.0044113
  • Peer Reviewed
• [Journal Article] Abnormal conformation and impaired degradation of NETs induced by propylthiouracil: Implication of disordered NETs in MPO-ANCA-associated vasculitis (2012)
  • Author(s): Nakazawa, D., Tomaru, U., Suzuki, A., Hasegawa, R., Kobayashi, T., Nishio, S., Kasahara, M. and Ishizu, A
  • Journal Title: Arthritis Rheum Volume: 64 Pages: 3779-3787
  • Peer Reviewed
• [Journal Article] PACAP and their receptors: ancestralorigins of today's highly diversified forms (2012)
  • Author(s): Ng, S. Y. L., Chow, B. K. C., Kasamatsu, J., Kasahara, M. and Lee, L. T. O. Agnathan VIP
  • Journal Title: PLoS ONE Volume: 7 Pages: e44691
  • DOI: Doi:10.1371/journal.pone.0044691.
  • Peer Reviewed
• [Journal Article] Immuno- genetics of the NKG2D ligand gene family (2012)
  • Author(s): Kasahara, M. and Yoshida, S
  • Journal Title: Immunogenetics Volume: 64 Pages: 855-867
  • Peer Reviewed
• [Journal Article] Proteasome subunit 5t expression in cervical ectopic thymoma (2012)
  • Author(s): Tomaru, U., Yamada, Y., Ishizu, A., Kuroda, T., Matsuno, Y. and Kasahara, M
  • Journal Title: J. Clin. Pathol Volume: 65 Pages: 858-859
  • Peer Reviewed
• [Journal Article] Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair (2012)
  • Author(s): Yoshida, S., Mohamed, R. H., Kajikawa, M., Koizumi, J., Tanaka, M., Fugo, K., Otsuka,N., Maenaka, K., Yagita, H., Chiba, H. and Kasahara, M
  • Journal Title: J. Immunol Volume: 188 Pages: 3972-3979
  • Peer Reviewed
• [Journal Article] Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities (2012)
  • Author(s): Tomaru, U., Takahashi, S., Ishizu, A., Miyatake, Y., Gohda, A., Suzuki, S., Ono, A., Ohara, J., Baba, T., Murata, S., Tanaka, K. and Kasahara, M
  • Journal Title: Am. J. Pathol Volume: 180 Pages: 963-972
  • Peer Reviewed
• [Journal Article] VLR-based adaptive immunity (2012)
  • Author(s): Boehm, T., McCurley, N., Sutoh, Y., Schorpp, M., Kasahara, M. and Cooper, M. D
  • Journal Title: Annu.Rev. Immunol Volume: 30 Pages: 203-220
  • Peer Reviewed
• [Journal Article] Comparative genomic analysis of the proteasome β5t subunit gene: implications for the origin and evolution of thymoproteasomes (2012)
  • Author(s): Sutoh, Y., Kondo, M., Ohta, Y., Ota, T., Tomaru, U., Flajnik, M. F. and Kasahara, M
  • Journal Title: Immunogenetics Volume: 64 Pages: 49-58
  • Peer Reviewed
• [Journal Article] 脊椎動物における抗原受容体の選択-相同リンパ球に発現される由来の異なる受容体 (2012)
  • Author(s): 須藤洋一,笠原正典
  • Journal Title: 化学と生物 Volume: 50(9) Pages: 624-626
  • Peer Reviewed
• [Journal Article] Expression of proteasome subunit 5t in thymic epithelial tumors (2011)
  • Author(s): Yamada, Y., Tomaru, U., Ishizu, A., Kiuchi, T., Marukawa, K., Matsuno, Y. and Kasahara, M
  • Journal Title: Am. J. Surg. Pathol Volume: 35 Pages: 1296-1304
  • Peer Reviewed
• [Journal Article] Serum-dependent, antibody and Fcγ receptor-mediated translocation of CD8 molecules from T cells to monocytes (2011)
  • Author(s): Iwasaki, S., Masuda, S., Baba, T., Tomaru, U., Katsumata, K., Kasahara, M. and Ishizu, A
  • Journal Title: Cytometry Volume: 79A Pages: 46-56
  • Peer Reviewed
• [Presentation] ヤツメウナギ由来可変性リンパ球受容体VLRCの結晶構造 (2013)
  • Author(s): 神田諒,須藤洋一,笠松純,笠原正典,前仲勝美,尾瀬農之
  • Organizer: 生物物理学会北海道支部会
  • Place of Presentation: 北海道大学理学部(札幌市)
  • Year and Date: 2013-03-05
• [Presentation] Inactivation of Skint1 in hominoids but not in Old World monkeys (2013)
  • Author(s): Mohamed, R. H., Sutoh, Y., Otsuka, N., Miyatake, Y. and Kasahara, M
  • Organizer: 第32回日本胸腺研究会
  • Place of Presentation: 北海道大学学術交流会館(札幌市)
  • Year and Date: 2013-02-09
• [Presentation] AB型胸腺腫におけるβ5tの発現 (2013)
  • Author(s): 山田洋介,外丸詩野,畑中佳奈子,石津明洋,木内隆之,三橋智子,松野吉宏,笠原正典
  • Organizer: 第32回日本胸腺研究会
  • Place of Presentation: 北海道大学学術交流会館(札幌市)
  • Year and Date: 2013-02-09
• [Presentation] Re-evaluation of thymoma classification through the distribution of a functional molecule. 5th Asia Pacific Lung Cancer Conference (2012)
  • Author(s): Matsuno, Y., Yamada, Y., Tomaru, U. and Kasahara, M
  • Organizer: 3rd International Thymic Malignancy Interest Group Annual Meeting
  • Place of Presentation: Fukuoka (Hilton Fukuoka Sea Hawk Hotel), Japan
  • Year and Date: 20121125-28
• [Presentation] 胎盤形成と妊娠維持へのNKG2Dリガンドの関与 (2012)
  • Author(s): 山本菜見子,大塚紀幸,宮武由甲子,吉田繁,笠原正典
  • Organizer: 第27回日本生殖免疫学会
  • Place of Presentation: 大阪医科大学(高槻市)
  • Year and Date: 2012-12-08
• [Presentation] Abnormal conformation and impaired degradation of neutrophil extracellular traps (NETs) induced by propylthiouracil (PTU): Implication of disordered NETs in MPO-ANCA-associated vasculitis(MPO-AAV) (2012)
  • Author(s): Nakazawa, D., Tomaru, U., Nishio, S., Atsumi, T., Kasahara, M. and Ishizu, A
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際展示場(神戸市)
  • Year and Date: 2012-12-07
• [Presentation] Contact with epithelial cells induces cancer stem cell-like properties in adult T-cell leukemia/lymphoma cells (2012)
  • Author(s): Miyatake, Y., Tomaru, U., Hall, W. W. and Kasahara, M
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際展示場(神戸市)
  • Year and Date: 2012-12-07
• [Presentation] Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair (2012)
  • Author(s): Rania, H. M., Yoshida, S., Miyatake, Y., Otsuka, N. and Kasahara, M
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2012-12-05
• [Presentation] Skewed T cell development in mice with aberrant expression of thymoproteasome (2012)
  • Author(s): Ohara, J., Tomaru, U., Ishizu, A., Konno, S., Suzuki, S., Murata, S., Tanaka, K. and Kasahara, M
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2012-12-05
• [Presentation] 原始的な脊椎動物ヤツメウナギから免疫系の進化を探る (2012)
  • Author(s): 須藤洋一,笠原正典
  • Organizer: 生化学会/生物物理学会/分子生物学会各北海道支部合同シンポジウム
  • Place of Presentation: 北海道大学学術交流会館(札幌市)
  • Year and Date: 2012-11-16
• [Presentation] 胎盤形成と妊娠維持へのNKG2Dリガンドの関与 (2012)
  • Author(s): 山本菜見子,大塚紀幸,宮武由甲子,吉田繁,笠原正典
  • Organizer: 第45回北海道病理談話会
  • Year and Date: 2012-10-13
• [Presentation] 上皮細胞は接着している成人T細胞白血病細胞を細胞静止状態にして保護する (2012)
  • Author(s): 宮武由甲子,外丸詩野,笠原正典
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(札幌市)
  • Year and Date: 2012-09-19
• [Presentation] 成人T細胞白血病における上皮細胞の潜在的役割 (2012)
  • Author(s): 宮武由甲子,Andre,L.A.O,Noreen,S.,大塚紀幸,外丸詩野,Hall,W.W.,笠原正典
  • Organizer: 第92回北海道医学大会
  • Place of Presentation: 北海道大学医学部学友会館フラテホール(札幌市)
  • Year and Date: 2012-09-08
• [Presentation] VLRC defines a population of lamprey lymphocytes distinct from that expressing VLRA or VLRB (2012)
  • Author(s): Sutoh, Y., Kasamatsu, J., Otsuka, N., Miyatake, Y. and Kasahara, M
  • Organizer: The 12th InternationalCongress of the International Society for Developmental and Comparative Immunology
  • Place of Presentation: Fukuoka (Hilton Fukuoka SeaHawk Hotel), Japan
  • Year and Date: 2012-07-10
• [Presentation] 肺腺癌に伴うトルソー症候群により肺高血圧症を合併した一剖検例 (2012)
  • Author(s): 今本鉄平,大塚紀幸,山田洋介,外丸詩野,高階太一,石津明洋,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-28
• [Presentation] 胸腺プロテアソームの発現異常がT細胞分化に与える影響について (2012)
  • Author(s): 小原次郎,外丸詩野,鈴木小百合,紺野沙織,村田茂穂,田中啓二,石津明洋,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-28
• [Presentation] 成人T細胞白血病(ATL)の病態における上皮細胞の役割の検討 (2012)
  • Author(s): 宮武由甲子,外丸詩野,NoreenSheehy,石津明洋,WilliamW.Hall,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-28
• [Presentation] プロテアソーム活性の低下が腫瘍微小環境に与える影響 (2012)
  • Author(s): 小野綾子,外丸詩野,石津明洋,小原次郎,紺野沙織,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-28
• [Presentation] プロテアソーム機能の低下とCOPDに対する病理作用 (2012)
  • Author(s): 山田洋介,外丸詩野,木内隆之,石津明洋,松野吉宏,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-28
• [Presentation] ヒト乳癌におけるNKG2Dリガンドの発現に関する検討 (2012)
  • Author(s): 藤田裕美,畑中豊,鈴木雄太,久保田佳奈子,大庭幸治,三橋智子,笠原正典,松野吉宏
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-27
• [Presentation] TNFα変換酵素(TACE)の過剰発現が糖および脂質代謝へ及ぼす影響 (2012)
  • Author(s): 松井由希,深谷進司,外丸詩野,渥美達也,笠原正典,石津明洋
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-26
• [Presentation] 創傷治癒におけるナチュラルキラー細胞活性化NKG2Dリガンドの役割 (2012)
  • Author(s): 吉田繁 Rania Hassan Mohamed,大塚紀幸,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-26
• [Presentation] 胎盤でのNKG2Dリガンド発現とその意義 (2012)
  • Author(s): 大塚紀幸,山本菜見子,近藤瑞穂,吉田繁,笠原正典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-26
• [Presentation] ヒト癌組織におけるNKG2Dリガンドの発現 (2011)
  • Author(s): 藤田裕美,畑中豊,大塚紀幸,須藤洋一,久保田加奈子,三橋智子,笠原正典,松野吉宏
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知芸術文化センター(名古屋市)
  • Year and Date: 20111003-05
• [Presentation] NKG2Dリガンドの創傷治癒における役割の解析 (2011)
  • Author(s): 笠原正典,Rania Mohamed,小泉潤,富居一範,大塚紀幸,吉田繁
  • Organizer: 第20回日本組織適合性学会大会
  • Place of Presentation: ツインメッセ静岡(静岡市)
  • Year and Date: 20110828-30
• [Presentation] 非古典的MHCクラスI分子の多様な機能 (2011)
  • Author(s): 笠原正典
  • Organizer: 第20回日本組織適合性学会大会
  • Place of Presentation: ツインメッセ静岡(静岡市)
  • Year and Date: 20110828-30
• [Presentation] 胸腺プロテアソームの系統発生 (2011)
  • Author(s): 笠原正典,須藤洋一,外丸詩野
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 海洋研究開発機構横浜研究所(横浜市)
  • Year and Date: 20110821-23
• [Presentation] Structure and function of variable lymphocyte receptors: an update. Symposium "Evolution and Diversity of Innate and Adaptive Immune Systems (2011)
  • Author(s): Kasahara, M., Sutoh, Y. and Kasamatsu, J
  • Organizer: International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya Congress Center,Nagoya ,Japan
  • Year and Date: 20110531-0605
• [Presentation] Characterization of adaptive immune receptors in hagfish (2011)
  • Author(s): Guo, P., Gartland, L. A., Li, J., Alder, M. N., Herrin, B. R., Sides, J. D., Kasahara, M. and Cooper, M. D.
  • Organizer: American Association of Immunologists Meeting, the Moscone Center
  • Place of Presentation: San Francisco, California, USA
  • Year and Date: 20110513-17
• [Presentation] ヒトNKG2Dリガンドの組織発現に関する免疫組織化学検討 (2011)
  • Author(s): 藤田裕美,須藤洋一,畑中豊,富居一範,大塚紀幸,松野吉宏,笠原正典
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20110428-30
• [Presentation] 体表組織におけるナチュラルキラー細胞活性化リガンドH60cの役割 (2011)
  • Author(s): 吉田繁,小泉潤,富居一範,近藤瑞穂,大塚紀幸,笠原正典
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20110428-30
• [Presentation] 母児間免疫寛容に関わるNKG2Dリガンド発現の解析 (2011)
  • Author(s): 大塚紀幸,近藤瑞穂,吉田繁,笠原正典
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20110428-30
• [Presentation] Blockade of interactions between NKG2D and an NKG2D ligand H60c delays wound healing (2011)
  • Author(s): Rania Mohamed,吉田繁,小泉潤,富居一範,大塚紀幸,笠原正典
  • Organizer: 第44回北海道病理談話会
  • Place of Presentation: 旭川市国際会議場(旭川市)
  • Year and Date: 2011-09-10
• [Book] John Wiley & Sons Ltd (2013)
  • Author(s): Kasahara, M
• [Book] 生化学事典 (2013)
  • Author(s): 笠原正典
  • Publisher: 朝倉書店(印刷中)
• [Book] 現代生物科学入門 (2011)
  • Author(s): 笠原正典
  • Total Pages: 159-197
  • Publisher: 岩波書店
• [Book] John Wiley & Sons Ltd (2011)
  • Author(s): Kasahara, M
• [Remarks]
  • URL: http://www.path1-hokudai.jp/path1/Welcome.html