Establishment of Peyer's patch M-cell-deficient mice to analyzeM-cell function

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659199
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Shintaro 東京大学, 医科学研究所, 助教 (80447333)
• Project Period (FY): 2011 – 2012
• Keywords: 発生病理 / M 細胞

Research Abstract

Although many of the biological features of microfold cells (M cells) have been known for many years, the molecular mechanisms of M-cell development and antigen recognition have remained unclear. Here, we report that transcription factor Spi-B was specifically expressed in M cells among non-hematopoetic lineages. Spi-B-deficient mice showed reducedexpression of most, but not all, other M cell-specific genes and M-cell surface markers. Whereas uptake of Salmonella Typhimurium via M cells was obviously reduced in Spi-B-deficient mice, the abundance of intra-tissue cohabiting bacteria was comparable between wild-type and Spi-B-deficient mice. These data indicate that there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.

Research Products

• [Journal Article] Nanogel-based PspA intranasal vaccine prevents invasive disease and nasal colonization by Streptococcus pneumoniae. (2013)
  • Author(s): Kong IG, Sato A, Yuki Y, Nochi T, Takahashi H, Sawada S, Mejima M, Kurokawa S, Okada K, Sato S, Briles DE, Kunisawa J, Inoue Y, Yamamoto M, Akiyoshi K, Kiyono H.
  • Journal Title: Infect Immun Volume: 81 Pages: 1625-1634
  • DOI: doi:10.1128/IAI.00240-13
• [Journal Article] Transcription factor Spi-B-dependent and -independent pathways for the development ofPeyer's patch M cells. (2012)
  • Author(s): Sato S, Kaneto S, Shibata N, Takahashi Y, Okura H, Yuki Y, Kunisawa J, Kiyono H.
  • Journal Title: Mucosal Immunol Volume: Dec 5
  • DOI: doi:10.1038/mi.2012.122
• [Journal Article] Lipocalin 2 bolsters innate and adaptive immune responses to blood-stage malaria infection by reinforcing host iron metabolism (2012)
  • Author(s): Zhao H, Konishi A, Fujita Y, Yagi M, Ohata K, Aoshi T, Itagaki S, Sato S, Narita H, Abdelgelil NH, Inoue M, Culleton R, Kaneko O, Nakagawa A, Horii T, Akira S, Ishii KJ, Coban C
  • Journal Title: CellHost Microbe Volume: 12 Pages: 705-716
  • DOI: doi:10.1016/j.chom.2012.10.010
• [Journal Article] Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors (2012)
  • Author(s): Kurashima Y, Amiya T, Nochi T, Fujisawa K, Haraguchi T, Iba H, Tsutsui H, Sato S, Nakajima S, Iijima H, Kubo M, Kunisawa J, Kiyono H
  • Journal Title: Nat Commun Volume: 3 Pages: 1034
  • DOI: doi:10.1038/ncomms2023
• [Journal Article] The mucosal immune system of the respiratory tract (2012)
  • Author(s): Sato S, Kiyono H
  • Journal Title: Curr Opin Virol Volume: 2 Pages: 225-232
  • DOI: doi:10.1016/j.coviro.2012.03.009.
• [Journal Article] The airway antigen sampling system: respiratory M cells as an alternative gateway for inhaled antigens (2011)
  • Author(s): Kim DY, Sato A, Fukuyama S, Sagara H, Nagatake T, Kong IG, Goda K, Nochi T, Kunisawa J, Sato S, Yokota Y, Lee CH, Kiyono H
  • Journal Title: J Immunol Volume: 186 Pages: 4253-4262
• [Presentation] Discovery of novel cell surface markers and two non-redundant pathways for the development of Perey' s patch M cells (2012)
  • Author(s): Shintaro Sato, Satoshi Kaneto, Naoko Shibata, Hideaki Okura, and Hiroshi Kiyono
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20121200