Study of bacterial adhering mechanism onto mammalian epithelial cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659225
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: IKE Fumio 独立行政法人理化学研究所, 独立行政法人理化学研究所, 専任研究員 (40183157)
• Co-Investigator(Kenkyū-buntansha): KOKUBO Toshiaki 独立行政法人放射線医学総合研究所, 研究基盤センター, 課長 (10425663)
• Research Collaborator: 松下 悟 独立行政法人放射線医学総合研究所, 研究基盤センター ; HATTORI Masahira 東京大学大学院新領域創成科学研究科, 大学院・新領域創成科学研究科・附属オーミクス情報センター, 教授 ; OSHIMA Kenshiro 東京大学, 大学院新領域創成科学研究科・附属オーミクス情報センター, 特任助教 ; MURATA Takehide 独立行政法人理化学研究所, 遺伝子材料開発室, 遺伝子材料開発室 ; OKUBO Masato 独立行政法人理化学研究所, 遺伝子材料開発室, テクニカルスタッフ ; KAJITA Ayako 独立行政法人理化学研究所, 実験動物開発室, 人材派遣
• Project Period (FY): 2011 – 2012

Research Abstract

The cilia-associated respiratory bacillus (CARB), a gram-negative extracellular bacterium, causes chronic respiratory disease in rodents. In this research, we got full genome sequence of CARB (about 1.44 Mbp long with around 1,200 coding sequences). Using this information, we also designed custom DNA array chip to analyze CARB's RNA expression when CARB attached to and grown on epithelial cells in vitro.

Research Products

• [Journal Article] Reverse Transcription-Loop-Mediated Isothermal Amplification for the De-tection of Rodent Coronaviruses (2013)
  • Author(s): Hanaki K*, Ike F, Hatakeyama R, Hirano N.
  • Journal Title: J. Virol. Meth Volume: 187 (2) Pages: 222-227
  • Peer Reviewed
• [Journal Article] An On-Site Serology Monitoring System Consisting of a Multiplex Microfluidic Chip Fabricated Using the Electrospray Deposition Method for Laboratory Mice (2012)
  • Author(s): Aoki H, Kaneko A, Kajita A, Yamagata Y, Ike F*, Kase H
  • Journal Title: J. Chem. Eng. Jap. Volume: 45 (7) Pages: 528-538
  • Peer Reviewed
• [Journal Article] 齧歯類のニューモシスチス感染症 (2012)
  • Author(s): 池 郁生
  • Journal Title: 実験動物ニュース Volume: 61(4) Pages: 47-50
• [Presentation] What is Rodent Pneumonic CAR bacillus? - From Full Genome Sequencing (2013)
  • Author(s): Ike F. Kajita A, Yoshiki A, Okubo M, Murata T, Oshima K, Hattori M and Kokubo T
  • Organizer: 12th FELASA SECAL Congress
  • Place of Presentation: Barcelona's International Convention Center, Barcelona, Spain
  • Year and Date: 20130610-13
• [Presentation] Culture Method Improvement of CAR Bacillus, Rodent Pneumonic Extracel-lular Bacteria, by Adding Methyl-cellulose (2013)
  • Author(s): Ike F
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ国際会議場, 千葉県千葉市
  • Year and Date: 20130318-20
• [Presentation] Biofilm Development by Rodent Pneu-monic Extracellular Bacteria (2012)
  • Author(s): Ike F and Kokubo T
  • Organizer: Biofilms 5
  • Place of Presentation: Espace Saint-Martin, Paris, France
  • Year and Date: 20121210-12
• [Presentation] CAR Bacillus Single Culture (2012)
  • Author(s): Ike F, Kajita A, Yoshiki A, Kokubo T
  • Organizer: 5th AFLAS Congress
  • Place of Presentation: Bangkok International Trade & Exhibition Centre, Bangkok, Thailand
  • Year and Date: 20121010-12
• [Presentation] Microbiological Status of Japanese Mouse Colony: From RIKEN BRC monitoring results (2012)
  • Author(s): Ike F and Yoshiki A
  • Organizer: 5th AFLAS Congress
  • Place of Presentation: Bangkok International Trade & Exhibition Centre, Bangkok, Thailand
  • Year and Date: 20121010-12
• [Presentation] Draft sequencing of rodent pneumonic CAR bacillus (2012)
  • Author(s): Ike F, Kajita A, Yoshiki A, Okubo M, Murata T, Oshima K, Hattori M, Kokubo T
  • Organizer: The 11th Awaji Interna-tional Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Con-ference Center, Awaji, Hyogo, Japan
  • Year and Date: 20120911-14
• [Presentation] Emerging microbiological contamina-tion and its detection techniques in mice; Information from the quarantine at a mouse bank (2012)
  • Author(s): Ike F
  • Organizer: 2012 KALAS International Symposium
  • Place of Presentation: Lote Buyeo Resort, Buyeo, Korea(招待講演)
  • Year and Date: 20120823-25
• [Presentation] Four-stage Model of CAR Bacillus infection In Vitro (2011)
  • Author(s): Ike F, Kokubo T, Matsushita S, Yoshiki A.
  • Organizer: 62nd AALAS National Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20111002-06
• [Presentation] Host-derived factor induces morpho-logical change in pneumonic extra-cellular bacteria (2011)
  • Author(s): Ike F, Matsushita S, Kokubo T
  • Organizer: IUMS 2011, XII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110906-10
• [Remarks]
  • URL: http://www.brc.riken.jp/lab/animal/