New classification of nociceptive sensory neurons and functional interaction between TRPV1 and TRPA1

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659323
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2012); National Institutes of Natural Sciences Okazaki Research Facilities (2011)
• Principal Investigator: TOMINAGA Makoto 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90260041)
• Project Period (FY): 2011 – 2012
• Keywords: 侵害受容器

Research Abstract

We found that capsiate, an ingredient contained in non-pungent capsicum, activates TRPA1. We found that Primary alcohols activate human TRPA1 channel in a carbon chain length-dependent manner. We found that two isothiocyanates from Wasabia japonica (6-MSITC, 6-MTITC) activate TRPA1. We found that 1,8-cineole, a TRPM8 agonist, is a novel natural antagonist of human TRPA1 upon intensive screening.

Research Products

• [Journal Article] Activation of TRPA1 by a non-pungent capsaicin-like compound, capsiate. Br. J. Pharmacol. (2012)
  • Author(s): Shintaku K, Uchida K, Suzuki Y, Zhou Y, Fushiki T, Watanabe T, Yazawa S, Tominaga M.
  • Journal Title: Br. J. Pharmacol Volume: 165 Pages: 1476-1486
  • DOI: doi:10.1111/j.1476-5381.2011.01634.x.
  • Peer Reviewed
• [Journal Article] Primary alcohols activate human TRPA1 channel in a carbon chain length-dependent manner. (2012)
  • Author(s): Komatsu T, Uchida K, Fujita F, Zhou Y, Tominaga M.
  • Journal Title: Pfluger Archiv. Eur. J. Physiol. Volume: 463 (4) Pages: 549-59
  • DOI: DOI:10.1007/s00424-011-1069-4
  • Peer Reviewed
• [Journal Article] Isothiocyanates from Wasabia japonica activate transient receptor potential ankyrin 1 channel. (2012)
  • Author(s): Uchida K, Miura Y, Nagai M & Tominaga M.
  • Journal Title: Chemical Senses Volume: 37 Pages: 809-818
  • DOI: DOI:10.1093/chemse/bjs065
  • Peer Reviewed
• [Journal Article] 1,8-cineole, a TRPM8 agonist, is a novel natural antagonist of human TRPA1 (2012)
  • Author(s): Takaishi M, Fujita F, Uchida K, Yamamoto S, Sawada M, Hatai C, Shimizu M & Tominaga M.
  • Journal Title: Molecular Pain Volume: 8 Pages: 86
  • DOI: DOI:11.1186/1744-8069-8-86
  • Peer Reviewed
• [Presentation] ワサビから抽出される種々のイソチオシアネートによるTRPA1の活性化 (2012)
  • Author(s): 内田邦敏、三浦陽介、永井雅、富永真琴、
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 愛知
  • Year and Date: 2012-09-20
• [Presentation] 辛くないトウガラシ成分カプシエイトによるTRPA1の活性化 (2011)
  • Author(s): 新宅健司、富永真琴
  • Organizer: 第33回日本疼痛学会
  • Place of Presentation: 愛知
  • Year and Date: 2011-07-23
• [Remarks]
  • URL: http://www.nips.ac.jp/cs