2013 Fiscal Year Final Research Report
Isolation and characterization of cross-reactive human monoclonal antibodies against human noroviruses.
| Project/Area Number |
23659338
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Fujita Health University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 抗ノロウイルス抗体 / ヒト型ファージ抗体 / 組織・血液型抗原 / 交叉反応性 |
|---|
| Research Abstract |
Three phage-displayed antibody libraries originating from healthy person(s) were screened using purified virus-like particles (VLPs) of strain Narita 104 (r104) or strain Chiba 407 (rCV,) as antigens. On screening with r104, 62 clones were isolated. Among these antibodies, 2 clones, 12A11 and 12B10, showed intra-genogroup cross-reactivity to genotypes 1, 3-7, 12, and 14, and genotypes 1, 4, 6, and 7 of genogroup II, respectively. On screening with rCV, 5 clones were isolated, 2 of which were cross-reactive. One, CV-2F5, reacted to genotypes 1-4, and 8 of genogroup I, and the other, CV-1A5, showed inter-genogroup cross-reactivity to all the VLPs employed in this study. The blocking activities of the monoclonal antibodies (mAbs) against the interaction of homotypic VLPs with histo-blood group antigens (HGBAs) were also assessed. 12B10 and CV-1A5 prevented the binding of the VLPs to HBGAs, suggesting that they are cross-reactive mAbs with neutralizing activity.
|
Research Products
(5 results)
-
-
-
[Presentation] Randomized placebo-controlled clinical trial of immunoglobulin Y as adjunct to standard supportive therapy for rotavirus-associated diarrhea among pediatric patients2012
Author(s)
Rahaman S,Higo-Moriguchi K, Htun K W,Taniguchi K, Icatlio FC, Tsuji T, Kodama Y, Nguyen SV, Umeda K, Oo HN, Myint YY, Htut T, Myint SS, Thura K, Thu HM, Fatmawati NND, Ogyma K
Organizer
46th Joint Working Conference on Viral Diseases, The Japan-United States Cooperative Medical Science Program
Place of Presentation
Japan (Sapporo)
Year and Date
20120000
-
-
