2013 Fiscal Year Final Research Report

New Transgenic Mouse System to Analyze Functional Mechanisms of Transcription Factors

Research Project

Project/Area Number	23659425
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Circulatory organs internal medicine
Research Institution	Nara Medical University
Principal Investigator	NAKAGAWA Osamu 奈良県立医科大学, 先端医学研究機構, 教授 (40283593)
Research Collaborator	HAYASHI Hisaki 奈良県立医科大学, 先端医学研究機構生命システム医科学分野, 循環器システム医科学研究室 (30533715) SAKABE Masahide 奈良県立医科大学, 先端医学研究機構生命システム医科学分野, 循環器システム医科学研究室 (00525983)
Project Period (FY)	2011 – 2013
Keywords	分子心臓病態学 / 転写調節因子 / 心血管系 / トランスジェニックマウス
Research Abstract	Transcription factors form multi-protein complexes with various partner proteins including non-DNA binding co-factors and chromatin remodeling enzymes, and regulate expression patterns of numerous target genes. In this study, we generated transgenic mouse lines that express a transcription factor containing Tandem Affinity Purification (TAP)-tag. These transgenic mice express TAP-tag proteins tissue-dependently in an inducible manner, and are applicable to various experiments such as mass spec analyses of transcriptional co-factors as well as ChIP-sequening of target genes. Such studies will help dissection of molecular mechanisms of in vivo transcriptional regulation.