2012 Fiscal Year Final Research Report
A study on the development of new strategy to control inflammation using molecular mechanism of a IL-17 high-producing cell line
| Project/Area Number |
23659500
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
膠原病・アレルギー・感染症内科学
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
USUI Takashi 京都大学, 医学研究科, 非常勤講師 (90362483)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | IL-17 / マイクロアレイ / エピジェネティック制御 / ChiP 解析 |
|---|
| Research Abstract |
IL-17 is an important cytokine in the pathogenesis of autoimmune inflammatory disease, including collagen disease. We established stable murine T cell clones which produce a large amount of IL-17 as well as its low producer from the same T cell line. Then we can analyzed its molecular mechanisms by DNA sequence, RNA (microarray) and epigenetic levels. Although we could not differences in DNA sequence in the promoter region and the expressions of transcriptional factors, we found methylationand de-methylation status were quite different in these clones. Furthermore, we found gene-X could control it. These findings will lead to new-generation strategy for controlling autoimmune inflammatory disease, including collagen disease.
|
-
[Journal Article] Three Groups in the 28 Joints for Rheumatoid Arthritis Synovitis - Analysis Using More than 17,000 Assessments in the KURAMA Database.2013
Author(s)
Terao C, Hashimoto M, Yamamoto K, Murakami K, Ohmura K, Nakashima R, Yamakawa N, Yoshifuji H, Yukawa N, Kawabata D, Usui T, Yoshitomi H, Furu M, Yamada R, Matsuda F, Ito H, Fujii T, Mimori T
-
Journal Title
PLoS One
Volume: 8(3)
Pages: e59341
-
[Journal Article] NEFA/nucleobindin-2 is a target autoantigen of the anti-Wa antibody and is associated with transfer RNA2012
Author(s)
Imura Y, Shirai Y, Nojima T, Nakashima R, Yamagata H, Miyachi K, Yoshifuji H, Kawabata D, Ohmura K, Usui T, Fujii T, Mimori T
-
Journal Title
Mod Rheumatol
Volume: 22(5)
Pages: 685-94
-
[Journal Article] Follistatin-related protein /follistatin-like 1 evokes an innate immune response via CD14 and toll-like receptor42012
Author(s)
Murakami K, Tanaka M, Usui T, Kawabata D, Shiomi A, Iguchi-Hashimoto M, Shimizu M, Yukawa N, Yoshifuji H, Nojima T, Ohmura K, Fujii T, Umehara H, Mimori T
-
Journal Title
FEBS Lett
Volume: 586(4)
Pages: 319-24
-
-
[Journal Article] Overexpression of a minimal domain of calpastatin suppresses IL-6 production and Th17 development via reduced NF- κB and increased STAT5 signals2011
Author(s)
Iguchi-Hashimoto M, Usui T, Yoshifuji H, Shimizu M, Kobayashi S, Ito Y, Murakami K, Shiomi A, Yukawa N, Kawabata D, Nojima T, Ohmura K, Fujii T, Mimori T
-
Journal Title
PLoS One
Volume: 6(10)
Pages: e27020
-
-
[Journal Article] Interferon-gamma release assay for diagnosing Mycobacterium tuberculosis infections in patients with systemic lupus erythematosus2011
Author(s)
Takeda N, Nojima T, Terao C, Yukawa N, Kawabata D, Ohmura K, Usui T, Fujii T, Ito Y, Iinuma Y, Mimori T
-
Journal Title
Lupus
Volume: 20(8)
Pages: 792-800
-
-
