Development of novel biomarker assay system for severe dengue virus infection.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23659508
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Nagasaki University
• Principal Investigator: YASUNAMI Michio 長崎大学, 熱帯医学研究所, 教授 (80244127)
• Project Period (FY): 2011 – 2012
• Keywords: 感染症 / 重症化予測 / バイオマーカー / デング出血熱 / 免疫測定 / 国際情報交換

Research Abstract

Dengue hemorrhagic fever, a severe sequela of dengue virus infection is a life-threatening condition unless proper treatments are not applied. In the present study, a commercially-available portable SPR device SPR01 (Optoquest, Ageo, Japan) was used for development of rapid quantitative assay of biomarkers for dengue hemorrhagic fever. Dengue virus non-structural protein NS1 and two chemokines, IP10 and CCL3 (MIP-1 alpha) were selected as candidates for such biomarkers. Quantitative assays for dengue virus NS1, IP10 and CCL3 were successfully designed and applied to plasma samples collected in hospitals in Ho Chi Minh City, Vietnam, for the evaluation of its usefulness.

Research Products

• [Journal Article] HLA-B*35 : 05 is a protective allele with a unique structure among HIV-1 CRF01_AE-infected Thais, in whom the B*57 frequency is low (2014)
  • Author(s): Mori M, Wichukchinda N, Miyahara R, Rojanawiwat A, Pathipvanich P, MaekawaT, Miura T, Goulder P, Yasunami M, Ariyoshi K, Sawanpanyalert P
  • Journal Title: AIDS Volume: 28(7) Pages: 959-967
  • DOI: 10.1097/QAD.0000000000000206.
  • Peer Reviewed
• [Journal Article] A novel high-throughput method for molecular serotyping and serotype-specific quantification of Streptococcus pneumoniae using a nanofluidic real-time PCR system (2014)
  • Author(s): Dhoubhadel BG, Yasunami M, Yoshida LM, Thi HA, Thi TH, Thi TA, Watanabe K, Suzuki M, Morimoto K, Dang DA, Ariyoshi K
  • Journal Title: J Med Microbiol Volume: 63 (Pt 4) Pages: 528-539
  • DOI: 10.1099/jmm.0.071464-0.
  • Peer Reviewed
• [Journal Article] HLA-DP gene polymorphisms and hepatitis B infection in the Japanese population (2012)
  • Author(s): Migita K, Abiru S, Ohtani M, Jiuchi Y, Maeda Y, Bae SK, Bekki S, Hashimoto S, Yesmembetov K, Nagaoka S, Nakamura M, Komori A, Ichikawa T, Nakao K, Yatsuhashi H, Ishibashi H, Yasunami M
  • Journal Title: Transl Res Volume: 160 Pages: 443-444
  • DOI: 10.1016/j.trsl.2012.06.003.
  • Peer Reviewed
• [Journal Article] The rs150311303 polymorphism in FcγRIIa enhances IgG binding capacity (2012)
  • Author(s): Omar AH, Shibata H, Yasunami M, Yamazaki A, Ofori MF, Akanmori BD, Shuaibu MN, Kikuchi M, Hirayama K
  • Journal Title: Scand J Immunol Volume: 76 Pages: 167-174
  • DOI: 10.1111/j.1365-3083.2012.02715.x
  • Peer Reviewed
• [Journal Article] Association of mast cell-derived VEGF and proteases in Dengue shock syndrome (2012)
  • Author(s): Furuta T, Murao LA, Lan NT, Huy NT, Huong VT, Thuy TT, Tham VD, Nga CT, Ha TT, Ohmoto Y, Kikuchi M, Morita K, Yasunami M, Hirayama K, Watanabe N
  • Journal Title: PLoS Negl Trop Dis Volume: 6 Pages: e1505
  • DOI: 10.1371/journal.pntd.0001505.
  • Peer Reviewed
• [Journal Article] Elevated Levels of Cell-Free Circulating DNA in Patients with Acute Dengue Virus Infection (2011)
  • Author(s): Tran TNH, Nguyen TH, Lyre AM, Nguyen TPL, Tran TT, Ha MT, Cao TPN, Vo VT, Tran VD, Kikuchi M, Yasunami M, Morita K, Vu TQH, Hirayama K
  • Journal Title: PLoS ONE Volume: 6(10) Pages: e25969
  • DOI: 10.1371/journal.pone.0025969.
  • Peer Reviewed
• [Presentation] Nha Trang Birth Cohort Study ---A Prospective Study for the Identification of Risk Factors for Childhood Infection/Infectious Diseases (2014)
  • Author(s): Michio Yasunami, Reiko Miyahara, Lay-Myint Yoshida, Hitomi Nakamura, Kensuke Takahashi, Vu Dinh Thiem,Trinh Xuan Mai, Bui Trong Chien, Dang Duc Anh, Le Huu Tho, Hiroyuki Moriuchi, Koya Ariyoshi
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections - 2014
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20140120-22
• [Presentation] What are we learning about hepatitis virus infection from the Nha Trang birth cohort study in Vietnam? (2014)
  • Author(s): Michio Yasunami
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections - 2014
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20140120-22
• [Presentation] A birth cohort study revealed that the susceptibility to acute lower respiratory infection was associated with TLR10-TLR1-TLR6 polymorphisms (2013)
  • Author(s): Michio Yasunami, Reiko Miyahara, Lay-Myint Yoshida, Hitomi Nakamura, Kensuke Takahashi, Trinh Xuan Mai, Bui Trong Chien, Vu Dinh Thiem, Le Huu Tho, Dang Duc Anh, Koya Ariyoshi
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 幕張
  • Year and Date: 20131211-13
• [Presentation] Cell type-specific, type I interferon-induced expression of APOBEC3G in HIV-exposed seronegatives (2013)
  • Author(s): Michio Yasunami, Nuanjun Wichukchinda, Akiko Takaki, Archawin Rojanawiwat, Panita Pathipvanich, Pathom Sawanpanyalert, Koya Ariyoshi
  • Organizer: The 11th International Congress on AIDS in Asia and the Pacific
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 20131118-22
• [Presentation] Nha Trang Birth Cohort Study : a population-based study for the identification of phenotype expression of genetic risk factors for childhood infectious diseases in Vietnam (2013)
  • Author(s): Michio Yasunami, Reiko Miyahara, Lay-Myint Yoshida, Hitomi Nakamura, Kensuke Takahashi, Vu Dinh Thiem, Trinh Xuan Mai, Bui Trong Chien, Dang Duc Anh, Le Huu Tho, Koya Ariyoshi
  • Organizer: The American Society of Human Genetics 63rd Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 20131022-26
• [Presentation] 東南アジアの臨床疫学フィールドから学ぶウイルス感染症学 (2012)
  • Author(s): 安波道郎
  • Organizer: 第60回日本ウイルス学会学術集会シンポジウム2「熱帯感染症」
  • Place of Presentation: 大阪
  • Year and Date: 20121113-15
• [Presentation] A Challenge to Clarify to What Extent the Human Genetic Variations Determine the Risk for Childhood Infection and Infectious Diseases---the NhaTrang Birth Cohort Study (2012)
  • Author(s): Michio Yasunami, Reiko Miyahara, Lay Myint Yoshida, Hitomi Nakamura, Kensuke Takahashi, Vu Thi Que Huong, Trinh Xuan Mai, Vu Dinh Thiem, Le Huu Tho, Dang Duc Anh, Hiroyuki Moriuchi and Koya Ariyoshi
  • Organizer: NhaTrang Birth Cohort Symposium
  • Place of Presentation: NhaTrang, Vietnam
  • Year and Date: 2012-11-28
• [Presentation] Launching population -based immunogenetic phenotype study in Vietnam (2011)
  • Author(s): Michio Yasunami
  • Organizer: SCIENTIFIC CONFERENCE PIHCMC
  • Place of Presentation: Ho Chi Minh City, Vietnam
  • Year and Date: 2011-11-17