An application of imaging mass spectrometry to human placentas

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659534
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: ITOH Hiroaki 浜松医科大学, 医学部附属病院, 教授 (70263085)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazunao 浜松医科大学, 医学部附属病院, 講師 (50456571)
• Co-Investigator(Renkei-kenkyūsha): HAYASAKA Takahiro 浜松医科大学, 分子イメージング先端研究センター, 助教 (90415927)
• Project Period (FY): 2011 – 2012
• Keywords: 胎児医学 / 成人病胎児起源説

Research Abstract

A matrix-assisted laser desorption ionization (MALDI)-based mass spectrometer provides clear two-dimensional molecular identification with highly sensitive mass spectrometry from mixtures of ions generated on tissue surfaces. We applied this technology to the molecular identification of phospholipids in the human term placenta and found that sphingomyelin (d18:1/16:0) and phosphatidylcholine (16:0/20:4) were distributed differently between stem and terminal villi. We further identified four phospholipids overexpressed in the terminal villi complicated with severe preeclampsia. Further study is ongoing.

Research Products

• [Journal Article] Cerebral oxygen saturation evaluated by near-infraredtime-resolved spectroscopy (TRS) in pregnant women during cesarean section -A promising new method of maternal monitoring (2013)
  • Author(s): Yamazaki K, Suzuki K, Itoh H, Muramatsu K, Nagahashi K, Tamura N, Uchida T, Sugihara K, Maeda H, Kanayama N.
  • Journal Title: Clinical Physiology and Functional Imaging Volume: 33 Pages: 109-116
  • DOI: DOI:10.1111/cpf.12001.
  • Peer Reviewed
• [Journal Article] Prevalence of inappropriate dietary supplement use among pregnant women in Japan (2013)
  • Author(s): Sato Y, Nakanishi T, Chiba T, Yokotani K, Ishinaga K, Takimoto H, Itoh H, Umegaki K
  • Journal Title: Asia Pac J Clin Nutr Volume: 22(1) Pages: 83-89
  • DOI: DOI:10.6133/apjcn.2013.22.1.08
  • Peer Reviewed
• [Journal Article] Possible contribution of fetal size and gestational age to myocardial tissue Doppler velocities in preterm fetuses (2013)
  • Author(s): Sekii K, Itoh H, Ogata T, Iwashima S
  • Journal Title: Eur J Obstet Gynecol Reprod Biol Volume: 167 Pages: 121
  • DOI: DOI:10.1016/j.ejogrb.2012.09.027
  • Peer Reviewed
• [Journal Article] Reduction in Levels of Maternal Complement during Delivery by Cesarean Section. (2012)
  • Author(s): Kato M, Itoh H, Nagahashi K, Izima M, Hirai K, Tamura M, Uchida T, Yaguchi C, Suzuki K, Sugihara K, Kanayama K.
  • Journal Title: J Obstet Gynaecol Res Volume: 38 Pages: 165-171
  • DOI: DOI:10.1111/j.1447-0756.2011.01661.x.
  • Peer Reviewed
• [Journal Article] Immunohistochemical Detection of Meconium in the Fetal Membrane, Placenta and Umbilical Cord (2012)
  • Author(s): Furuta N, Yaguchi C, Itoh H, Morishima Y, Tamura N, Kato M, Uchida T, Suzuki K, Sugihara K, Kawabata Y, Suzuki N, Sasaki T,Horiuchi K, and Kanayama N.
  • Journal Title: Placenta Volume: 33 Pages: 24-30
  • DOI: DOI:10.1016/j.placenta.2011.10.007
  • Peer Reviewed
• [Journal Article] Successful living donor liver transplantation for fulminant hepatic failure that manifested immediately after cesarean delivery (2012)
  • Author(s): Horikoshi Y, Itoh H, Kikuchi S, Uchida T, Suzuki K, Sugihara K, Kanayama N, Mori A, and Uemoto S.
  • Journal Title: ASIO J Volume: 58 Pages: 174-176
  • DOI: DOI:10.1097/MAT.0b013e3182444ed4
  • Peer Reviewed
• [Journal Article] Fetal myocardial tissue Doppler indices before birth physiologically change in proportion to body size adjusted for gestational age in low-risk term pregnancies (2012)
  • Author(s): Sekii K, Takamichi Ishikawa T, Ogata T, Itoh H, Iwashima S
  • Journal Title: Early Hum Dev Volume: 88 Pages: 517-523
  • DOI: DOI:10.1016/j.earlhumdev.2011.12.014.
  • Peer Reviewed
• [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation (2012)
  • Author(s): Suzukin K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
  • Journal Title: Clin Exp Obstet Gynecol Volume: XXXIX(3) Pages: 293-298
  • Peer Reviewed
• [Journal Article] Deterioration of myocardial tissue Doppler indices in a case of fetal hydrothorax as a promising indication for clinical intervention before the development of nonimmune hydrops fetalis. (2012)
  • Author(s): Sekii K, Itoh H, Ogata T, Iwashima S
  • Journal Title: Arch Gynecol Obstet Volume: 256 Pages: 1079-1080
  • DOI: DOI:10.1007/s00404-012-2375-6.
  • Peer Reviewed
• [Journal Article] Neonatal exposure to leptin reduces glucose tolerance in adult mice (2011)
  • Author(s): Itoh H, Yura S, Sagawa N, Kanayama N, Konishi I and HBC Study Team
  • Journal Title: Acta Physiologica Volume: 202 Pages: 159-164
  • DOI: DOI:10.1111/j.1748-1716.2011.02268.x.
  • Peer Reviewed
• [Journal Article] Branched-chain amino acid supplemented diet during maternal food restriction prevents developmental hypertension in adult rat offspring. (2011)
  • Author(s): Fuji T, Yura S, Tatsumi K, Mogami H, Fujita K, Kondoh E, Kakui K, Aoe S, Itoh H, Sagawa N, Fujii S, Konishi I.
  • Journal Title: J Dev Orig Health Dis Volume: 2 Pages: 176-183
  • DOI: DOI:http://dx.doi.org/10.1017/S204017441100002X
  • Peer Reviewed
• [Journal Article] The HBC Study Team. Age-specific 3-month cumulative incidence of postpartum depression:The Hamamatsu Birth Cohort (HBC) Study. (2011)
  • Author(s): Matsumoto K, Tsuchiya KJ, Itoh H, Kanayama N, Suda S, Matsuzaki H, Iwata Y, Suzuki K, Nakamura K, Mori N, Takei N
  • Journal Title: J Affect Disord Volume: 133 Pages: 607-610
  • DOI: DOI:10.1016/j.jad.2011.04.024.
  • Peer Reviewed
• [Journal Article] A rapid activated protein C sensitivity test as a diagnostic marker for a suspected venous thromboembolism in pregnancy and puerperium (2011)
  • Author(s): Hirai K, Sugimura M, Ohashi R, Suzuki K, Itoh H, Sugihara K, Kanayama N.
  • Journal Title: Gynecol Obstet Invest Volume: 72 Pages: 55-62
  • DOI: DOI:10.1159/000322880
  • Peer Reviewed
• [Journal Article] A case of spontaneous rupture of a uterine superficial varicose vein in midgestation. (2011)
  • Author(s): Nakaya Y, Itoh H, Muramatsu K, Otome M, Kobayashi Y, Hirai K, Uchida T, Suzuki K, Sugihara K, and Kanayama N.
  • Journal Title: J Obstet Gynaecol Res Volume: 37 Pages: 1149-1153
  • DOI: DOI:10.1111/j.1447-0756.2010.01489.x.
  • Peer Reviewed
• [Journal Article] Japan Society of Obstetrics and Gynecology; Japan Association of Obstetricians and Gynecologists. Guidelines for obstetrical practice in Japan: Japan Society of Obstetrics and Gynecology (JSOG) and Japan Association of Obstetricians and Gynecologists (JAOG) 2011 edition. (2011)
  • Author(s): Minakami H, Hiramatsu Y, Koresawa M, Fujii T, Hamada H, Iitsuka Y, Ikeda T, Ishikawa H, Ishimoto H, Itoh H, Kanayama N, Kasuga Y, Kawabata M, Konishi I, Matsubara S, Matsuda H, Murakoshi T, Ohkuchi A, Okai T, Saito S, Sakai M, Satoh S, Sekizawa A, Suzuki M, Takahashi T, Tokunaga A, Tsukahara Y, Yoshikawa H
  • Journal Title: J Obstet Gynaecol Res Volume: 37(9) Pages: 1174-1197
  • DOI: DOI:10.1111/j.1447-0756.2011.01653.x.
  • Peer Reviewed
• [Presentation] Comparison of phospholipid molecualr species of human placenta between preeclampsia and normal case by Imaging Mass Spectrometry (2013)
  • Author(s): Yamazaki K, Hayasaka T, Muramatsu K, Nagahashi K, Yaguchi C, Itoh H, Kanayam N
  • Organizer: 60th Annual Meeting of Society for Gynecologic Investigation (SGI).
  • Place of Presentation: Orland, USA
  • Year and Date: 20130320-0323