Transcriptome Analysis of Schizophrenia

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659563
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Nagoya University
• Principal Investigator: OZAKI Norio 名古屋大学, 医学系研究科, 教授 (40281480)
• Co-Investigator(Renkei-kenkyūsha): OHNO Kinji 名古屋大学, 大学院・医学系研究科, 教授 (80397455)
• Project Period (FY): 2011 – 2012
• Keywords: トランスクリプトーム解析 / 統合失調症 / 分子精神医学 / ゲノム医学 / バイオインフォマティックス

Research Abstract

Transcriptome analysis of lymphoblastoid cell line derived fromschizophrenic patients and healthy controls was performed to investigate dysregulationof alternative splicing by comparing the profiles observed. We found the subtle differences in gene and exon level analysis between schizophrenic patients and healthy controls. The top hit genes were tested using pathway and hierarchical clustering analysis. In addition we observed a number of regulatory SNPs (expression quantitative trait loci-eQTL) by integrating data from genotype and expression wise analysis. As a result, the genes which expression was affected by the disease status may be associated with molecular mechanisms that contribute to the pathophysiology of schizophrenia.

Research Products

• [Journal Article] DPP6 as a candidate gene for neuroleptic-induced tardive dyskinesia (2013)
  • Author(s): Tanaka S, Syu A, Ishiguro H, Inada T, Horiuchi Y, Ishikawa M, Koga M, Noguchi E, Ozaki N, Someya T, Kakita A, Takahashi H, Nawa H,Arinami T
  • Journal Title: Pharmacogenomics J Volume: 13(1) Pages: 27-34
  • DOI: DOI:10.1038/tpj.2011.36
  • Peer Reviewed
• [Journal Article] Effects of aging on the morphologies of Heschl's gyrus and the superior temporal gyrus in schizophrenia: A postmortem study (2012)
  • Author(s): Torii Y, Iritani S, Sekiguchi H, Habuchi C,Hagikura M, Arai T, Ikeda K, Akiyama H, Ozaki N
  • Journal Title: Schizophr Res Volume: 134 Pages: 137-142
  • DOI: DOI:10.1016/j.schres.2011.10.024
  • Peer Reviewed
• [Journal Article] Association of SNPs linked to increased expression of SLC1A1 with schizophrenia (2012)
  • Author(s): Horiuchi Y, Iida S, Koga M, Ishiguro H, Iijima Y, Inada T, Watanabe Y, Someya T, Ujike H, Iwata N, Ozaki N, Kunugi H, Tochigi M, Itokawa M, Arai M, Niizato K, Iritani S, Kakita A, Takahashi H, Nawa H, Arinami T
  • Journal Title: Am J Med Genet B Neuropsychiatr Genet Volume: 159B (1) Pages: 30-7
  • DOI: DOI:10.1002/ajmg.b.31249
  • Peer Reviewed
• [Journal Article] Genome-wide association study of schizophrenia in a Japanese population (2011)
  • Author(s): Ikeda M, Aleksic B, その他20名, Ozaki N,O'Donovan MC, Iwata N
  • Journal Title: Biol Psychiatry Volume: 69 (5) Pages: 472-478
  • DOI: DOI:10.1016/j.biopsych.2010.07.010
  • Peer Reviewed
• [Journal Article] Resequencing and Association Analysis of the KALRN and EPHB1 Genes And Their Contribution to Schizophrenia Susceptibility (2011)
  • Author(s): Kushima I, Nakamura Y, Aleksic B, Ikeda M, ItoY, Shiino T, Okochi T, Fukuo Y, Ujike H, SuzukiM, Inada T, Hashimoto R, Takeda M, Kaibuchi K,Iwata N, Ozaki N
  • Journal Title: Schizophr Bull Volume: 38(3) Pages: 552-560
  • DOI: DOI:10.1093/schbul/sbq118
  • Peer Reviewed
• [Journal Article] Vulnerability in early life to changes in the rearing environment plays a crucial role in the aetiopathology of psychiatric disorders (2011)
  • Author(s): Niwa M, Matsumoto Y, Mouri A, Ozaki N,Nabeshima T
  • Journal Title: Int J Neuropsychopharmacol Volume: 14 (4) Pages: 459-477
  • DOI: DOI:10.1017/S1461145710001239
  • Peer Reviewed
• [Journal Article] Impairment of the tyrosine hydroxylase neuronal network in the orbitofrontal cortex of a genetically modified mouse model of schizophrenia (2011)
  • Author(s): Sekiguchi H, Iritani S, Habuchi C, Torii Y,Kuroda K, Kaibuchi K, Ozaki N
  • Volume: 1392
  • Pages: 47-53
  • DOI: DOI:10.1016/j.brainres.2011.03.058
  • Peer Reviewed
• [Journal Article] Loss of Function Studies in Mice and Genetic Association Link Receptor Protein Tyrosine Phosphatase alpha to Schizophrenia (2011)
  • Author(s): Takahashi N, Nielsen KS, Aleksic B, Petersen S,Ikeda M, Kushima I, Vacaresse N, Ujike H, IwataN, Dubreuil V, Mirza N, Sakurai T, Ozaki N,Buxbaum JD, Sap J
  • Volume: 70 (7)
  • Pages: 626-635
  • DOI: DOI:10.1016/j.biopsych.2011.06.016
  • Peer Reviewed
• [Presentation] Schizophrenia associated polymorphism regulates PTPRA transcript expression in lymphoblastoid cell lines (2012)
  • Author(s): Akira Yoshimi, Nagahide Takahashi, Branko Aleksic, Itaru Kushima, Masashi Ikeda, Hiroshi Ujike, Takeshi Sakurai, Joseph D. Buxbaum, Jan Sap, Nakao Iwata, Norio Ozaki
  • Organizer: WCPG 2012
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 20121014-18
• [Presentation] Myelin-related abnormality of schizophrenia: genetic, imaging and postmortem study (2012)
  • Author(s): Ozaki N
  • Organizer: the 15th Pacific Rim College of Psychiatrists Scientific Meeting (PRCP 2012) Symposium Genetics of Schizophrenia
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2012-10-25
• [Presentation] White matter abnormalities in schizophrenia: genetic, imaging and postmortem study (2012)
  • Author(s): 尾崎紀夫
  • Organizer: Neuro2012
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-20
• [Presentation] Protein tyrosine phosphatase alufa as novel candidate molecule for the etiopathology of schizophrenia: Genetic analysis and biological implications (2011)
  • Author(s): 尾崎紀夫
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] Genome study of Japanese schizophrenia: GWAS, CNV and Rare variants: Symposium: Neuroscience and neurochemistry in Japan (2011)
  • Author(s): Ozaki N
  • Organizer: WFSBP Congress 2011
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2011-05-31