2013 Fiscal Year Final Research Report
Study of hydroxymethlated DNA and TET gene family in cancer
| Project/Area Number |
23659610
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
SATO FUMIAKI 京都大学, 医学(系)研究科(研究院), 准教授 (20467426)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 乳癌 / ハイドロキシメチル化 |
|---|
| Research Abstract |
The aim of this study is to clarify the significance of hydroxymethyl CpG (hmCpG) in breast cancer cells. The hmCpG is thought to be the first step in demethylation mechanism of methylated CpG, and to play an important roles in epigenetic gene regulation in cancer. In this study, to screen differentially hydroxymethylated regions (DHMR), we conducted to methylation microarray analysis to identify differentially hypomethylated regions, using ATCC's breast cancer cell line panels. According to the detailed analysis, we identified subtype specific hypomethylated regions in Luminal type and triple negative breast cancers, which would be candidate regions of DHMR.
On the other hand, we established a gene induction system of TET1/2/3 gene families, using Lenti-virus vector system. It will help the further functional analysis of TET1/2/3 genes in breast cancer cells.
|
