Establishment of immune cell therapy using iPS-derived MDSC

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659629
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: LI Xiaokang 独立行政法人国立成育医療研究センター, 研究所, 室長 (60321890)
• Project Period (FY): 2011 – 2012
• Keywords: 移植 / 再生医療 / 遺伝子 / 細胞・組織

Research Abstract

To establish a novel method to generate myeloid-derived suppressor cells(MDSCs) from mouse induced pluripotent stem (iPS). We demonstrated that the iPS cell-derived MDSCs (iPS-MDSCs) possessed the characteristics of MDSCs including the morphology of typical BM-derived MDSC; low expression of the surface molecular, Ia, CD11c, and CD40; capacity of suppress the allogeneic T cell responses. Also we set up a unique mouse GvHD model (B6 to BDF1) for valuation the iPS-MDSCs function by immune cell therapy.

Research Products

• [Journal Article] Non-myeloablative conditioning is sufficient to induce mixed chimerism and subsequent acceptance of donor specific cardiac and skin grafts. (2013)
  • Author(s): Liu C, Zhu P, Saito T, Isaka Y, Nagahara Y, Zhuang J, Li Xiao K
  • Journal Title: Int Immunopharmacol Volume: 16(3) Pages: 392-8
• [Journal Article] Bone marrow derived conventional, but not cloned, mesenchymal stem cells suppress lymphocyte proliferation and prevent graft-versus-host disease in rats. (2012)
  • Author(s): Kitazawa Y, Li Xiao K, Xie L, Zhu P, Kimura H, Takahara S
  • Journal Title: Cell Transplant Volume: 21(2) Pages: 581-90
• [Journal Article] Deletion of CD98hc in T cells results in cardiac allograft acceptance by increasing regulatory T cells. (2012)
  • Author(s): Liu Z, Hou J-G, Chen J-J, Tsumura H, Ito M, Ito Y, Hu X, Li Xiao K
  • Journal Title: Transplantation Volume: 93(11) Pages: 1116-24
• [Journal Article] Identification of a novel biomarker gene set with sensitivity and specificity to distinguish between allograft rejection and tolerance. (2012)
  • Author(s): Xie L, Ichimaru N, Morita M, Chen JJ, Zhu P, Wang JH, Urbanellis P, Shalev I, Nagao S, Sugioka A, Zhong L, Nonomura N, Takahara S, Levy GA, Li Xiao K
  • Journal Title: Liver Transplant Volume: 18 Pages: 444-454
• [Presentation] マウス肝移植モデルを用いた免疫寛容機序の解明 (2012)
  • Author(s): 李小康,森田美和,杉岡篤
  • Organizer: 第48回日本移植学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20120920-22
• [Presentation] A novel siRNA delivery system specifically silencing target gene to induce permanent acceptance of mouse cardiac allograft (2012)
  • Author(s): Zhang Q, Cai SJ, Hou JG, Yazawa K, Ichimaru N, Kobayashi M, Higuchi S, Uno A, Ando H, Sakurai K, Adachi Y, Ohno N,Xu JH, Li Xiao K, Takahara S
  • Organizer: 24^<th> International Congress of the Transplantation Society
  • Place of Presentation: Berlin, Germany
  • Year and Date: 20120715-19
• [Presentation] The requirement of the myeloid-derived suppressor cells for spontaneous acceptance after liver transplantation in mice (2011)
  • Author(s): Cai SJ, Morita M, Kitajima Y, Zhang Q, Hou JG, Kimura H, Lu L, Li Xiao K
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 幕張
  • Year and Date: 20111127-29