Noveltechnology for imuse cell induction.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659648
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: MORI Masaki 大阪大学, 医学系研究科, 教授 (70190999)
• Co-Investigator(Kenkyū-buntansha): EGUCHI Hidetoshi 大阪大学, 医学系研究科, 助教 (90542118) ; ISHII Hideshi 大阪大学, 医学系研究科, 教授 (10280736)
• Project Period (FY): 2011 – 2012
• Keywords: 再生医学 / 外科学 / ES 細胞

Research Abstract

Given that multilineage differentiating stress enduring (Muse) cells exist in adult skin fibroblasts and bone marrow stroma and possess stress tolerance and endogenous pluripotency, we studied the possible induction of Muse-like cells by miRNAs (iMuse cells). The iMuse cells have a potentially usefulness for the regenerative medicine as safety and efficiency. While expression of SSEA-3 showed specifically the relationship of representative stem markers as well as with mesenchymal stem cell/Muse cell markers in normal and cancer cells. Further study would be necessary for the final clinical setting.

Research Products

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• [Journal Article] Intraoperative versus extended antimicrobial prophylaxis after gastric cancer surgery: a phase 3, open-label, randomised controlled, non-inferiority trial (2012)
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  • Journal Title: Oncol Rep Volume: 28(6) Pages: 2221-2226
• [Journal Article] Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic miRNA clusters (2012)
  • Author(s): Nishida N.Nagahara M., Sato T., Mimori K., Sudo T., Tanaka F., Shibata K., Ishii H., Sugihara K., Doki Y., Mori M
  • Journal Title: Clin Cancer Res. Volume: 18(11) Pages: 3054-3070
• [Journal Article] MicroRNA-10b is a prognostic indicator in colorectal cancer and confers resistance to the chemotherapeutic agent 5-fluorouracil in colorectal cancer cells (2012)
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• [Journal Article] Emerging methods for preparing iPS cells. (2012)
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• [Journal Article] Let-7 expression is significant determinant of response to chemotherapy through the regulation of IL-6/STAT3 pathway in esophageal squamous cell carcinoma (2012)
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  • Journal Title: Clin Cancer Res Volume: 18(18) Pages: 5144-5153
• [Journal Article] Biological and clinical availability of adipose-derived stem cells for pelvic dead space repair (2012)
  • Author(s): Takahashi H., Haraguchi N.Nishikawa S., Miyazaki S., Suzuki Y., Mizushima T., Nishimura J., Takemasa I., Yamamoto H., Mimori K., Ishii H., Doki Y., Mori M
  • Journal Title: Stem Cells Transl Med. Volume: 1(11) Pages: 803-810
• [Journal Article] DOK2 as a marker of poor prognosis of patients with gastric adenocarcinoma after curative resection (2012)
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• [Journal Article] Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma (2012)
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  • Journal Title: Br J Cancer. Volume: 106(5) Pages: 947-954
• [Journal Article] MicroRNA-372 is associated with poor prognosis in colorectal cancer (2012)
  • Author(s): Yamashita S.Yamamoto H., Mimori K., Nishida N., Takahashi H., Haraguchi N., Tanaka F., Shibata K., Sekimoto M., Ishii H., Doki Y., Mori M.
  • Journal Title: Oncology Volume: 82(4) Pages: 205-212
• [Journal Article] Oncofetal protein, IMP-3, a potential marker for prediction of postoperative peritoneal dissemination in gastric adenocarcinoma (2012)
  • Author(s): Okada K.Fujiwara Y., Nakamura Y., Takiguchi S., Nakajima K., Miyata H., Yamasaki M., Kurokawa Y., Takahashi T., Mori M., Doki Y
  • Journal Title: J Surg Oncol Volume: 105(8) Pages: 780-785
• [Journal Article] Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma (2012)
  • Author(s): Tomimaru Y.Eguchi H., Nagano H., Wada H., Kobayashi S., Marubashi S., Tanemura M., Tomokuni A., Takemasa I., Umeshita K., Kanto T., Doki Y., Mori M
  • Journal Title: J Hepatol Volume: 56(1) Pages: 167-175
• [Journal Article] IGFBP7 downregulation is associated with tumor progression and clinical outcome in hepatocellular carcinoma (2012)
  • Author(s): Tomimaru Y.Eguchi H., Wada H., Kobayashi S., Marubashi S., Tanemura M., Umeshita K., Kim T., Wakasa K., Doki Y., Mori M., Nagano H
  • Journal Title: Int J Cancer Volume: 130(2) Pages: 319-327
• [Journal Article] A case of simultaneous transvaginal NOTES gastrectomy and vaginal hysterectomy in a patient with gastric submucosal tumor and uterine prolapse (2012)
  • Author(s): Hirota M.Nakajima K., Hara J., Takahashi T., Yamasaki M., Tsutsui T., Kobayashi E., Kurokawa Y., Miyata H., Takiguchi S., Mori M., Doki Y
  • Journal Title: Asian J Endosc Surg Volume: 5(4) Pages: 168-171
• [Journal Article] Role of the Hypoxia-Related Gene, JMJD1A, in Hepatocellular Carcinoma: Clinical Impact on Recurrence after Hepatic Resection. (2012)
  • Author(s): Yamada D.Kobayashi S., Yamamoto H., Tomimaru Y., Noda T., Uemura M., Wada H., Marubashi S., Eguchi H., Tanemura M., Doki Y., Mori M., Nagano H
  • Journal Title: Ann Surg Oncol Volume: 19(3) Pages: 355-364
• [Journal Article] Genomic copy number of a carcinogenic single nucleotide polymorphism at 8q24 in non-risk allele colorectal cancer associated with insulin growth factor 2 receptor expression. (2012)
  • Author(s): Takahashi Y.Mimori K., Yamamoto K., Watanabe M., Tanaka J., Kudo SE., Sugihara K., Hase K., Mochizuki H., Kusunoki M., Yamada K., Shimada Y., Moriya Y., Mori M
  • Journal Title: J Gastroenterol Hepatol Volume: 3 Pages: 95-99
• [Journal Article] Genotoxic therapy stimulates error-prone DNA repair in dormant hepatocellular cancer stem cells. (2012)
  • Author(s): Nishikawa S.Ishii H., Haraguchi N., Kano Y., Fukusumi T., Ohta K., Ozaki M., Sakai D., Satoh T., Nagano H., Doki Y., Mori M
  • Journal Title: Exp Ther Med Volume: 3(6) Pages: 959-962
• [Journal Article] microRNA-based cancer cell reprogramming technology (Review). (2012)
  • Author(s): Nishikawa S.Ishii H., Haraguchi N., Kano Y., Fukusumi T., Ohta K., Ozaki M., Dewi DL., Sakai D., Satoh T., Nagano H., Doki Y., Mori M.
  • Journal Title: Exp Ther Med Volume: 4(1) Pages: 8-14
• [Journal Article] Transcriptomic study of dormant gastrointestinal cancer stem cells (2012)
  • Author(s): Nishikawa S.Dewi D., Ishii H., Konno M., Haraguchi N, Kano Y., Fukusumi T., Ohta K., Noguchi Y., Ozaki M., Sakai D., Satoh T., Doki Y., Mori M
  • Journal Title: Int J Oncol Volume: 41(3) Pages: 979-984
• [Journal Article] Increased risk for CRC in diabetic patients with the nonrisk allele of SNPs at 8q24 (2012)
  • Author(s): Ishimaru S.Mimori K., Yamamoto K., Inoue H., Imoto S., Kawano S., Yamaguchi R., Sato T., Toh H., Iinuma H., Maeda T., Ishii H., Suzuki S., Tokudome S., Watanabe M., Tanaka J., Kudo SE., Sugihara K., Hase K., Mochizuki H., Kusunoki M., Yamada K., Shimada Y
  • Journal Title: Ann Surg Oncol Volume: 19(9) Pages: 2853-2858
• [Journal Article] Deficiency of N-acetylgalactosamine in O-linked oligosaccharides of IgA is a novel biologic marker for Crohn's disease (2012)
  • Author(s): Inoue T. Iijima H., Tajiri M., Shinzaki S., Shiraishi E., Hiyama S., Mukai A., Nakajima S., Iwatani H., Nishida T., Mizushima T., Yasui T., Isaka Y., Kanto T., Tsujii M., Miyoshi E., Wada Y., Takehara T.
  • Journal Title: Inflamm Bowel Dis Volume: 18(9) Pages: 1723-1734
• [Journal Article] Absence of CD71 transferrin receptor characterizes human gastric adenosquamous carcinoma stem cells (2012)
  • Author(s): Ohkuma M.Haraguchi N., Ishii H., Mimori K., Tanaka F., Kim HM., Shimomura M., Hirose H., Yanaga K., Mori M
  • Journal Title: Ann Surg Oncol Volume: 19.(4) Pages: 1357-1365
• [Journal Article] Aberrant DNA methylation associated with aggressiveness of gastrointestinal stromal tumour (2012)
  • Author(s): Okamoto Y.Sawaki A., Ito S., Nishida T., Takahashi T., Toyota M., Suzuki H., Shinomura Y., Takeuchi I., Shinjo K., An B., Ito H., Yamao K., Fujii M., Murakami H., Osada H., Kataoka H., Joh T., Sekido Y., Kondo Y.
  • Journal Title: Gut Volume: 61(3) Pages: 392-401
• [Journal Article] Increased CD13 expression reduces reactive oxygen species, promoting survival of liver cancer stem cells via an epithelial-mesenchymal transition-like phenomenon. (2012)
  • Author(s): Kim HM.Haraguchi N., Ishii H., Ohkuma M., Okano M., Mimori K., Eguchi H., Yamamoto H., Nagano H., Sekimoto M., Doki Y., Mori M.
  • Journal Title: Ann Surg Oncol. Volume: 3 Pages: 539-548
• [Journal Article] Upregulation of nuclear PA28γexpression in cirrhosis and hepatocellular carcinoma (2012)
  • Author(s): Kondo M.Moriishi K., Wada H., Noda T., Marubashi S., Wakasa K., Matsuura Y., Doki Y., Mori M., Nagano H
  • Journal Title: Exp Ther Med Volume: 3(3) Pages: 379-385
• [Journal Article] A new PICTure of nucleolar stress (2012)
  • Author(s): Suzuki A.Kogo R., Kawahara K., Sasaki M., Nishio M., Maehama T., Sasaki T., Mimori K., Mori M.
  • Journal Title: Cancer Sci. Volume: 103(4) Pages: 632-637
• [Journal Article] Prevention of GVHD and graft rejection by a new S1P receptor agonist, W-061, in rat small bowel transplantation (2012)
  • Author(s): Song J. Hagiya H., Kurata H., Mizuno H., Ito T.
  • Journal Title: Transpl Immunol Volume: 26(2-3) Pages: 163-170
• [Journal Article] PLOD2 induced under hypoxia is a novel prognostic factor for hepatocellular carcinoma after curative resection. (2012)
  • Author(s): Noda T. Yamamoto H., Takemasa I., Yamada D., Uemura M., Wada H., Kobayashi S., Marubashi S., Eguchi H., Tanemura M., Umeshita K., Doki Y., Mori M., Nagano H
  • Journal Title: Liver Int Volume: 32(1) Pages: 110-118
• [Journal Article] Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance (2012)
  • Author(s): Hashiguchi Y. Nishida N., Mimori K., Sudo T., Tanaka F., Shibata K., Ishii H., Mochizuki H., Hase K., Doki Y., Mori M.
  • Journal Title: Int J Oncol. Volume: 40(5) Pages: 1477-1482
• [Journal Article] ErbB receptor tyrosine kinase/NF-κB signaling controls mammosphere formation in human breast cancer (2012)
  • Author(s): Hinohara K.Kobayashi S., Kanauchi H., Shimizu S., Nishioka K., Tsuji E., Tada K., Umezawa K., Mori M., Ogawa T., Inoue J., Tojo A., Gotoh N.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109(17) Pages: 6584-6589
• [Journal Article] Hypoxia and TP53 deficiency for induced pluripotent stem cell-like properties in gastrointestinal cancer. (2012)
  • Author(s): Hoshino H.Nagano H., Haraguchi N., Nishikawa S., Tomokuni A., Kano Y., Fukusumi T., Saito T., Ozaki M., Sakai D., Satoh T., Eguchi H., Sekimoto M., Doki Y., Mori M., Ishii H
  • Journal Title: Int J Oncol Volume: 40(5) Pages: 1423-1430
• [Journal Article] I/II study of S-1 plus cisplatin combined with peptide vaccines for human vascular endothelial growth factor receptor 1 and 2 in patients with advanced gastric cancer (2012)
  • Author(s): Masuzawa T.Fujiwara Y., Okada K., Nakamura A., Takiguchi S., Nakajima K.,Miyata H., Yamasaki M., Kurokawa Y., Osawa R., Takeda K., Yoshida K., Tsunoda T., Nakamura Y., Mori M., Doki Y.
  • Journal Title: Int J Oncol Volume: 41(4) Pages: 1297-1304
• [Journal Article] Biased usage of T cell receptor β-chain variable region genes of Wilms' tumor gene (WT1)-specific CD8+ T cells in patients with solid tumors and healthy donors (2012)
  • Author(s): Mori Moto S.Oka Y., Tsuboi A., Tanaka Y., Fujiki F., Nakajima H., Hosen N., Nishida S., Nakata J., Nakae Y., Maruno M., Myoui A., Enomoto T., Izumoto S., Sekimoto M., Kagawa N., Hashimoto N., Yoshimine T., Oji Y., Kumanogoh A., Sugiyama H
  • Journal Title: Cancer Sci. Volume: 103(3) Pages: 408-414
• [Journal Article] Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma. (2012)
  • Author(s): Yokobori T.Mimori K., Iwatsuki M., Ishii H., Tanaka F., Sato T., Toh H., Sudo T., Iwaya T., Tanaka Y., Onoyama I., Kuwano H., Nakayama KI., Mori M.
  • Journal Title: Int J Oncol Volume: 41(1) Pages: 253-259
• [Presentation] 教育講演、さまざまなストレスに対処する為に機能するオートファジー機構 (2012)
  • Author(s): 森正樹
  • Organizer: 第16回 日本統合医療学会
  • Place of Presentation: 大阪
  • Year and Date: 20121208-09
• [Presentation] パネルディスカッション1、大腸癌の悪性度診断と臨床応用 (2012)
  • Author(s): 森正樹、冨田尚裕
  • Organizer: 第67回 日本大腸肛門病学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20121116-17
• [Presentation] 学術セミナー7、がん医療における新しい治療パラダイム (2012)
  • Author(s): 森正樹
  • Organizer: 第50回日本癌治療学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20121025-27
• [Presentation] New Treatment Development Based on Cancer Stem Cell Theory (2012)
  • Author(s): Mori M
  • Organizer: 2012 International Symposium on Recent Advances in Stem Cells and Cancer & The 8th Annual Meeting of Taiwan Society for Stem Cell Research
  • Place of Presentation: 高雄
  • Year and Date: 20121013-14
• [Presentation] S5-6 新規の細胞周期依存性 RhoGAP 「Celmobilin」によるヒト癌細胞の遊走と浸潤の制御 (2012)
  • Author(s): 賀川義規、西川恵三、前田栄、松本真司、奥崎大介、三森功士、山本浩文、関本貢嗣、菊池章石井秀始、土岐祐一郎、森正樹、石井優
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] LS23 消化器系腫瘍における癌幹細胞研究の今後の展望 (2012)
  • Author(s): 森正樹
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] シンポジウム1 「癌発生と進展のメカニズム」、消化器癌幹細胞マーカーの開発と治療・診断への応用 (2012)
  • Author(s): 原口直紹、西川晋平、太田勝也、石井秀始、山本浩文、永野浩昭、土岐祐一郎、森正樹
  • Organizer: 第21回日本癌病態治療研究会
  • Place of Presentation: 群馬
  • Year and Date: 20120706-07
• [Presentation] W1-2 FACS を応用した生体イメージング関連分子の解析 (2012)
  • Author(s): 賀川義規、西川恵三、前田栄、菊田順一、太田勝也、西村潤一、竹政伊知朗、水島恒和、池田正孝、山本浩文、関本貢嗣、石井秀始、土岐祐一郎、森正樹、石井優
  • Organizer: 第22回日本サイトメトリ学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20120629-30
• [Presentation] O2-3癌の細胞周期蛍光イメージングによる抗腫瘍薬の効果ポイントの可視化 (2012)
  • Author(s): 賀川義規、前田栄、太田勝也、菊田順一、西川恵三、西村潤一、竹政伊知朗、水島恒和、池田正孝、山本浩文、関本貢嗣、石井秀始、土岐祐一郎、森正樹、石井優
  • Organizer: 第22回日本サイトメトリ学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20120629-30
• [Presentation] 乳癌骨髄中の CD47発現と乳癌サブタイプにおける予後・再発予測因としての意義 (2012)
  • Author(s): 永原誠、三森功士、中川剛士、佐藤隆宣、杉原健一、森正樹
  • Organizer: 第20回 日本乳癌学会学術総会
  • Place of Presentation: 熊本
  • Year and Date: 20120628-30
• [Presentation] ランチョンセミナー2、分子標的治療薬の臨床薬理 (2012)
  • Author(s): 森正樹
  • Organizer: 第16回 日本がん分子標的治療学会
  • Place of Presentation: 福岡
  • Year and Date: 20120627-29
• [Presentation] ランチョンセミナーI、癌幹細胞をめぐる研究の展開 (2012)
  • Author(s): 森正樹
  • Organizer: 第33回 癌免疫外科研究会
  • Place of Presentation: 横浜
  • Year and Date: 20120517-18
• [Presentation] 2. 消化管 stem cellの新たな展開 (2012)
  • Author(s): 森正樹、渡辺守
  • Organizer: 第98回 日本消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 20120419-21
• [Presentation] S2-4 消化器癌における癌幹細胞研究の現状と今後の展開 (2012)
  • Author(s): 原口直昭、石井秀始、森正樹
  • Organizer: 第98回 日本消化器病学会総会
  • Place of Presentation: S2-4 消化器癌における癌幹細胞研究の現状と今後の展開
  • Year and Date: 20120419-21
• [Presentation] 教育講演I 最近の癌研究から分かったはっとすること (2012)
  • Author(s): 森正樹
  • Organizer: 第20回 日本大腸検査学会九州支部会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-08-04