2012 Fiscal Year Final Research Report
Establishment of chondrocyte induction from iPS cells using a fluorescent reporter system.
| Project/Area Number |
23659704
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAITO Taku 東京大学, 医学部附属病院, 特任准教授 (30456107)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Naoshi 東京大学, 医学部附属病院, 講師 (10361495)
MORIZAKI Yutaka 東京大学, 医学部附属病院, 助教 (30508099)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 骨 / 軟骨代謝学 |
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| Research Abstract |
We established fluorescent reporter iPS cells using Col2a1- and Prx1-promoter, and confirmed the fluorescent signal by stimulation of retinoic acid or BMP. We used the iPS cells to screen various factors for chondrogenic differentiation, and identified insulin, TGF-beta, inhibitor of the Notch signaling and the Wnt signaling. We are researching the optimal combination of these factors to induce chondrocyte from iPS cells.
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