2012 Fiscal Year Final Research Report
Clarification of molecular basis underlying acceleration of bone formation
| Project/Area Number |
23659712
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IMAI Yuuki 東京大学, 分子細胞生物学研究所, 特任講師 (10423873)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 骨 / 骨粗鬆症 / 骨細胞 / エピゲノム |
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| Research Abstract |
MC3T3E1 cells treated with differentiation medium could not express osteocyte marker genes. Therefore, we focused on epigenetic regulation during osteocyte differentiation. ChIP analyses revealed that methylation levels of H3K4 and K27 on transcriptional start sites of osteocyte marker genes were bivalent status, suggesting that demethylation of H3K27 might to be essential for the differentiation from osteoblast to osteocyte. To examine physiological impact of this phenomenon, we generated flox mice of Kdm6A, demethylace for H3K27.
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Research Products
(34 results)
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[Journal Article] Vitamin D receptor in osteoblasts is a negative regulator of bone mass control2013
Author(s)
Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K,Sato T, Kawano H, Aihara K, Nakamichi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S,Imai Y.
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Journal Title
Endocrinology
Volume: 154(3)
Pages: 1008-20
Peer Reviewed
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[Journal Article] JMJD5, a JmjC-domain-containingprotein, negatively regulates osteoclastogenesis through facilitating NFATc1 protein degradation.2012
Author(s)
Youn MY, Yokoyama A, Fujiyama- Nakamura S, Ohtake F, Minehata KI, Yasuda H, SuzukiT, Kato S, Imai Y.
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Journal Title
J Biol Chem
Volume: 87(16)
Pages: 12994-3004
Peer Reviewed
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[Journal Article] GlcNAcylation of histone H2B facilitates its monoubiquitination2011
Author(s)
Fujiki R, Hashiba W, Sekine H, Yokoyama A, Chikanishi T, Ito S, Imai Y, Kim JH, He HH, Igarashi K,Kanno J, Ohtake F, Kitagawa H, Roeder R, Brown M, Kato S
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Journal Title
Nature
Volume: 480(7378)
Pages: 557-60
Peer Reviewed
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[Journal Article] Signal-sensing activation of a histone lysine demethylase complex2011
Author(s)
Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S
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Journal Title
Nat Cell Biol
Volume: 13(6)
Pages: 669-76
Peer Reviewed
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