2012 Fiscal Year Final Research Report
Visualization of the process from generation to exocytosis of secretory granules by expression of fluorescent dye-binding proteins
| Project/Area Number |
23659875
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 口腔生理学 / ライブイメージング |
|---|
| Research Abstract |
To visualize the process from generation to exocytosis of secretory granules, we established an expression system of the fluorescent dye-binding protein HaloTag in salivary gland cells. We found that a HaloTag protein having only a signal peptide sequence was sorted into secretory granules of salivary glands. We succeeded in live imaging experiments of stimulus-dependent exocytosis by labeling secretory granules with fluorescent HaloTag ligands.
|
