Serum-free medium for dental regenerative tissue engineering

2011 Fiscal Year Final Research Report

• Project/Area Number: 23659918
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KATO Yukio 広島大学, 医歯薬学総合研究科, 教授 (10112062)
• Project Period (FY): 2011
• Keywords: 血清 / 幹細胞 / 無血清培地 / 歯 / 再生

Research Abstract

Ex vivo expansion and differentiation of dental epithelial cells and dental mesenchymal cells are required for tooth regeneration. We developed serum-free medium of dental pulp cells, and this many facillital development of dental tissue enginnering.

Research Products

• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic actor Bim in human oral cancer HSC-3 cells (2012)
  • Author(s): Wu Y, Sato F, Sato H, Jin D, BhawalUK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H
  • Journal Title: Biomedical Research Volume: 33(2)
  • Peer Reviewed
• [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts (2012)
  • Author(s): Sato F, Sato H, Jin D, Bhawal UK, WuY, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H
  • Journal Title: human mesenchymal stem cell, and in mouse liver Corresponding Volume: 419(2) Pages: 441-6
  • DOI: DOI:10.1016/j.bbrc.2012.02.076
  • Peer Reviewed
• [Journal Article] Regulation of basic helix-loop-helixtranscription factors Dec1 and Dec2 byRORα and their roles inadipogenesis (2012)
  • Author(s): Ozaki N, Noshiro M, Kawamoto T, Nakashima A, Honda K, Fukuzaki-Dohi U, Honma S, FujimotoK, Tanimoto K, Tanne K, Kato Y
  • Journal Title: Genes to Cells Volume: 17 Pages: 109-121
  • DOI: DOI:10.1111/j.1365-2443.2011.01574.x
  • Peer Reviewed
• [Journal Article] Feasibility and limitations of the round robin test for assessment of in vitro chondrogenesis evaluation protocol in a tissue-engineered medical product (2011)
  • Author(s): Yokoi M, Hattori K, Narikawa K, Ohgushi H, Tadokoro M, Hoshi K, Takato T, Myoui A, Nanno K, Kato Y, Kanawa M, Sugawara K, Kobo T, Ushida T
  • Journal Title: J Tissue Eng Regen Med., Article first published on line Pages: 460
  • DOI: DOI:10.1002/term.460
  • Peer Reviewed
• [Journal Article] The effect of synthetic octacalcium phosphate in a collagen scaffold on the osteogenicity of mesenchymal stem cells (2011)
  • Author(s): Kawai T., Anada T., Masuda T., Honda Y., Sakai Y., Kato Y., Kamakura S., Echigo S., Suzuki O
  • Journal Title: European Cells and Materials Volume: 22 Pages: 124-136
  • Peer Reviewed
• [Journal Article] Impact of Zinc Fingers and Homeoboxes 3(ZHX3) on the Regulation of Mesenchymal Stem Cell Osteogenic Differentiation (2011)
  • Author(s): Suehiro F, Nishimura M, Kawamoto T, Kanawa M, Yoshizawa Y, Murata H, Kato Y
  • Journal Title: Stem Cells Dev Volume: 20(9) Pages: 1539-47
  • DOI: DOI:10.1089/scd.2010.0279
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cell-induced cranial suture-like gap in rats (2011)
  • Author(s): Kaku M, Tai M, Kawata T, Fujita T, Motokawa M, Ohtani J, Sakai Y, KatoY, Tanne K.
  • Journal Title: Plast Reconstr Surg Volume: 127(1) Pages: 69-77
  • DOI: DOI:10.1097/PRS.0b013e3181f95849
  • Peer Reviewed
• [Journal Article] PERIOD1(PER1) hasanti-apoptotic effects, and PER3 haspro-apoptotic effects during cisplatin(CDDP) treatment in human gingival cancer CA9-22 cells (2011)
  • Author(s): Sato F, Wu Y, Bhawal UK, Liu Y, Imaizumi T, Morohashi S, Kato Y, Kijima H
  • Journal Title: Eur J Cancer Volume: 47(11) Pages: 1747-58
  • DOI: DOI:10.1016/j.ejca.2011.02.025
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factorsDEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells (2011)
  • Author(s): Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Morohashi S, Kato Y, Kijima H
  • Journal Title: Int J Mol Med Volume: 27(4) Pages: 491-5
  • DOI: DOI:10.3892/ijmm.2011.617
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates cyclin D1 (2011)
  • Author(s): Bhawal UK, Sato F, Arakawa Y, Fujimoto K, Kawamoto T, Tanimoto K, Ito Y, Sasahira T, Sakurai T, KobayashiM, Kashima I, Kijima H, Kuniyasu H, Abiko Y, Kato Y, Sato S
  • Journal Title: J Pathol Volume: 224(3) Pages: 420-9
  • DOI: DOI:10.1002/path.2878
  • Peer Reviewed
• [Presentation] Serum-Free Cultures for Human Mesenchymal Stem Cells using Defined Culture Surfaces. 1st Meeting of Surabaya Regenerative Medicine and Stem Cell Centre(SRMSC) (2011)
  • Author(s): Yukio Kato
  • Organizer: 2nd Meeting of Indonesia Association of Tissue Bank(IATB), SYMPOSIUM
  • Place of Presentation: Surabaya, Indonesia
  • Year and Date: 20110922-23
• [Presentation] 細胞表面抗原を指標とした口腔内からの骨前駆細胞の分取 (2011)
  • Author(s): 鎌田浩一、藤本勝巳、西村正宏、末廣史雄、貞森紳丞、赤川安正、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 20110513-14
• [Presentation] 間葉系幹細胞の軟骨分化におけるDEC2の役割 (2011)
  • Author(s): 笹本智子、藤本勝巳、金輪真佐美、河本健、能城光秀、道田将彦、尾崎徳継、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 20110513-14
• [Presentation] 間葉系幹細胞の増殖・分化ならびにインテクリン発現に対する転写因子GATA6の作用 (2011)
  • Author(s): 道田将彦、河本健、能城光秀、藤本勝巳、金輪真佐美、尾崎徳継、笹本智子、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 20110513-14
• [Presentation] 核内受容体RORαによる時計遺伝子DEC1, DEC2の発現調節と脂肪分化 (2011)
  • Author(s): 尾崎徳継、能城光秀、河本健、福崎麗、藤本勝巳、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 20110513-14
• [Patent(Industrial Property Rights)] 動物細胞培養キット、動物細胞の培養方法及び動物細胞の選択培養方法 (2012)
  • Inventor(s): 加藤幸夫、平田伊佐雄、金輪真佐美
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 特許、特願2011-084119
  • Filing Date: 2012-04-05
• [Patent(Industrial Property Rights)] 間葉系幹細胞の均質性識別方法、その方法を利用して得られる均質間葉系幹細胞 (2012)
  • Inventor(s): 加藤幸夫、金輪真佐美、五十嵐晃、原真依子、辻紘一郎
  • Industrial Property Rights Holder: 広島大学、(株)ツーセル
  • Industrial Property Number: 特許、特許第4958094号
  • Acquisition Date: 2012-03-30