2012 Fiscal Year Final Research Report

Effect of periodontopathic factors on osteoblastic differentiation of rat bone-marrow cells cultured under simulated microgravity

Research Project

Project/Area Number	23659979
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Periodontal dentistry
Research Institution	The University of Tokushima
Principal Investigator	NAGATA Toshihiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10127847)
Co-Investigator(Kenkyū-buntansha)	INAGAKI Yuji 徳島大学, 病院, 助教 (50380019) BANDO Mika 徳島大学, 病院, 医員 (10510000)
Project Period (FY)	2011 – 2012
Keywords	模擬無重力 / 3D クリノスタット / 骨芽細胞 / 歯周病原性因子
Research Abstract	This study investigated the effect of periodontopathic factors on osteoblastic differentiation of cultured rat bone-marrow cells under simulated microgravity. Experimental conditions were made up using 3D-clinostat for the simulated microgravity and lipopolysaccharide from P. gingivalis(P-LPS) for the periodontopathic factor. Osteoblastic differentiation of bone marrow cells was progressed by the microgravity, showing increases of bone-associated gene expression and bone-nodule formation in culture. These markers were inhibited by the addition of P-LPS. DNA-microarray analysis revealed an increase of gene expression of caspase, a molecule associated with endoplasmic reticulum stress. These results suggest that P-LPS may affect on osteoblastic differentiation under the microgravity condition.