2013 Fiscal Year Final Research Report
Analysis of adaptive evolution using an automated culture system of E. coli
| Project/Area Number |
23680030
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Bioinformatics/Life informatics
|
|---|
| Research Institution | Institute of Physical and Chemical Research (2012-2013)
Osaka University (2011) |
|---|
Principal Investigator |
FURUSAWA Chikara 独立行政法人理化学研究所, 生命システム研究センター, チーリーダー (00372631)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 進化 / 大腸菌 / オートメーション / トランスクリプトーム / ゲノム |
|---|
| Research Abstract |
Phenotype-genotype relationship in bacterial adaptive evolution still remains unclear. In this study, by using experimental evolution of bacterial cells, we quantified phenotypic change and genetic change during adaptive evolution to various stress conditions. For this purpose, first we developed an automated culture system by which we can maintain hundreds of independent culture series of bacterial cells. Then, by using this automated system, we performed experimental evolution of E. coli under various stress conditions, including acid, detergent, and antibiotic stresses, to obtain tolerant strains for these stresses. Expression changes and genetic mutations in the tolerant strains were analyzed by microarray experiments and next-gen sequencers, respectively. The results demonstrated that a part of adaptive phenotypic changes cannot be explained by fixed genetic mutations, suggesting that an unknown non-genetic mechanism plays a role in the dynamics of adaptive evolution.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Remarks] 大野聡,古澤力,清水浩,第22回生物工 学論文賞授賞, 2014年9月(受賞内定)
