Production of somatic cell nuclear transferred mice by regulations of X-linked gene expression

2014 Fiscal Year Final Research Report

• Project/Area Number: 23680048
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: INOUE KIMIKO 独立行政法人理化学研究所, バイオリソースセンター, 専任研究員 (70360500)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 体細胞核移植クローン / エピジェネティクス / 遺伝子発現

Outline of Final Research Achievements

Although somatic cell nuclear transfer (SCNT) is sole reproductive engineering technique to allow us to produce individuals from somatic cells, their developmental efficiency is usually less than 5%. In this study, we aimed at practical applications of this technique and attempted to develop procedures of gene regulations by non-genetic modification to improve low developmental efficiency of SCNT. As a results, we obtained following conclusions. (1) mRNA introduction of a transcription factor activated in fertilized embryos could improve developmental efficiency of SCNT embryos in vitro. (2) RNA knock down of Xist gene that triggered X chromosome inactivation in female somatic cells could remarkably ameliorate impaired developmental efficiency into term offsprings of SCNT embryos.

Free Research Field

発生工学