2013 Fiscal Year Final Research Report
Development of artificial organelle through the controlled cellular uptake of polyion complex vesicles
| Project/Area Number |
23685037
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyushu University (2013)
The University of Tokyo (2011-2012) |
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Principal Investigator |
KISHIMURA Akihiro 九州大学, 工学(系)研究科(研究院), 准教授 (70422326)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | ポリイオンコンプレックス / ベシクル / ブロック共重合体 / 人工オルガネラ / ドラッグデリバリーシステム / 酵素 / ナノリアクター |
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| Research Abstract |
We have so far developed polyion complex vesicles (PICsomes) with well-controlled size in the size ranging from 100 to 400 nm. PICsomes are characterized by their biocompatibility and semipermeable vesicle membrane. Therefore, enzyme-loaded PICsomes are promising for enzyme-based nanoreactors, which can work inside of cells as an artificial organelle. In this research project, we tried to develop "artificial organelle"utilizing PICsomes. The preparation method of nano-reactors was successfully established through encapsulation of enzymes in PICsomes without deactivation. Also, we investigated the interaction between chemically-modified PICsomes and cells, and evaluated and found major factors for further cellular uptake. Results indicated that degree of cross-linking of PIC membrane and deformability of PICsomes contribute cellular uptake to some extent.
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[Book] 有機機能材料基礎から応用まで2014
Author(s)
松浦和則, 角五彰, 岸村顕広, 佐伯昭紀, 竹岡敬和, 内藤昌信, 中西尚志, 舟橋正浩, 矢貝史樹
Publisher
講談社 (分担執筆;第10章、第12章担当), (ISBN978-4-06-156802-0)
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