Mechanical and thermal properties of living organisms at the mesoscopic scale

2013 Fiscal Year Final Research Report

• Project/Area Number: 23687021
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: SUZUKI Madoka 早稲田大学, 重点領域研究機構, 准教授 (40350475)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 共焦点顕微鏡 / 生物物理 / 分子モーター / 細胞・組織 / 生体分子

Research Abstract

(i) Force (work) and (ii) temperature (heat) are frequently considered as macroscopic parameters. Here we developed novel methods to handle these physical parameters at the single cell scale, and tried to discover biologically important phenomena. (i) A novel cell-sheet system was developed. We examined the response of a cell-sheet to external mechanical stimulus under an optical microscope. (ii) We found the cells respond to heat pulses. Also, we developed novel temperature probes to study single cell thermogenesis.

Research Products

• [Journal Article] A Nanoparticle-Based Ratiometric and Self-Calibrated Fluorescent Thermometer for Single Living Cells (2014)
  • Author(s): Takei, Y., Arai, S., Murata, A, Takabayashi, M., Oyama, K., Ishiwata, S., Takeoka, S. and Suzuki, M.
  • Journal Title: ACS Nano Volume: 8(1) Pages: 198-206
  • DOI: 10.1021/nn405456e
• [Journal Article] 歩くナノ温度計 (2013)
  • Author(s): 鈴木団、大山廣太郎、石渡信一
  • Journal Title: 生物物理 Volume: 53(3) Pages: 158-159
• [Journal Article] Walking nanothermometers : Spatiotemporal temperature measurement of transported acidic organelles in single living cells (2012)
  • Author(s): Oyama, K., Takabayashi, M., Takei, Y., Arai, S., Takeoka, T, Ishiwata, S. and Suzuki, M.
  • Journal Title: Lab Chip Volume: 12 Pages: 1591-1593
• [Journal Article] Mass Spectrometric Screening of Ligands with Lower Off-rate from a Clicked-based Pooled Library (2012)
  • Author(s): Arai, S., Hirosawa, S., Oguchi, Y., Suzuki, M., Murata, A., Ishiwata, S. and Takeoka, S
  • Journal Title: ACS Comb. Sci Volume: 14(8) Pages: 451-455
• [Journal Article] Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients (2012)
  • Author(s): Oyama, K., Mizuno, A., Shintani, S.A., Itoh, H., Serizawa, T., Fukuda, N., Suzuki, M. and Ishiwata, S.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 417 Pages: 607-612
• [Journal Article] Fast temperature measurement following single laser-induced cavitation inside a microfluidic gap. Quinto-Su, P.A.
  • Author(s): Suzuki, M. and Ohl, C.-D
  • Journal Title: Sci. Rep Volume: (in press)
• [Presentation] Thermometry in aqueous solution at single cell-scale using fluorescent nanothermometers (2014)
  • Author(s): Suzuki, M
  • Organizer: 第91回日本生理学会大会(The 91st Annual Meeting of the Physiological Society of Japan)
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 20140316-19
  • Invited
• [Presentation] Microscopic thermometry in a living cell by fluorescent nanoprobes (2014)
  • Author(s): Suzuki, M
  • Organizer: Japan-A*STAR Advanced Diagnostics Forum
  • Place of Presentation: Singapore
  • Year and Date: 2014-03-24
  • Invited
• [Presentation] Single cell-scale thermometry by fluorescent temperature reporters (2013)
  • Author(s): Suzuki, M
  • Organizer: 7th International Symposium on Nanomedicine (ISNM 2013)
  • Place of Presentation: Kyushu Institute of Technology, Kitakyushu, Japan
  • Year and Date: 20131107-09
  • Invited
• [Presentation] Temperature measurement in the scale of single cell with fluorescent temperature reporters. MBI Seminar, Mechanobiology Institute (2013)
  • Author(s): Suzuki, M
  • Organizer: National University of Singapore
  • Place of Presentation: Singapore
  • Year and Date: 2013-09-17
  • Invited
• [Presentation] 一匹の細胞で温度を扱うこと&擬似組織系で細胞の力学負荷応答を見ること (2013)
  • Author(s): 鈴木団
  • Organizer: 「細胞機能究明の最前線3」理研セミナー
  • Place of Presentation: 東京
  • Year and Date: 2013-06-20
  • Invited
• [Presentation] 光学顕微鏡を用いた局所熱励起・局所温度測定 (2011)
  • Author(s): 鈴木団
  • Organizer: 第10回国際バイオEXPO
  • Place of Presentation: 東京
  • Year and Date: 20110629-0701
  • Invited
• [Remarks] 報道関連情報、早稲田大プレスリリース「水中で触れずに細胞の中の温度を測れるレシオ型ナノ温度計を報道関連情報、開発ミクロンサイズの温度変化を測定するナノ温度計の手法を発展」2014年1月17日
• [Remarks] 報道関連情報、日経産業新聞「水中の細胞温度触れず正確測定早大が装置開発」2014年1月21日
• [Remarks] 報道関連情報、マイナビニュース「早稲田、水中で触れずに細胞の温度を測定する「レシオ型ナノ温度計」開発」2014年1月28日
• [Remarks] 報道関連情報、早稲田大プレスリリース「細胞内を歩くナノ温度計を開発ミクロンサイズの細胞内小器官内部の温度変化を世界で初めて測定」2012年3月15日
• [Remarks] 報道関連情報、マイナビニュース「早大、細胞内の局所的で微小な温度変化を測定可能な「歩くナノ温度計」開発」2012年3月14日
• [Remarks] 報道関連情報、日刊工業新聞「細胞内で0.3度C差測定早大ナノ粒子の温度計」2012年3月19日
• [Remarks] 報道関連情報、科学新聞「細胞内を歩く「ナノ温度計」開発ミクロンサイズの細胞内小器官内部の温度変化-早大が世界で初めて測定-」2012年3月23日