2014 Fiscal Year Final Research Report
Selective degradation of proteins and organelles during early embryogenesis
Project/Area Number |
23687027
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Cell biology
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Research Institution | Gunma University |
Principal Investigator |
SATO Miyuki 群馬大学, 生体調節研究所, 准教授 (70321768)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Keywords | オートファジー / エンドサイトーシス / リソソーム / 分解 / 受精 / 線虫 / ミトコンドリア |
Outline of Final Research Achievements |
Fertilization triggers cell remodeling from each gamete to a totipotent zygote. Using C. elegans as a model system, we have shown that lysosomal degradation pathways play important roles in cellular remodeling during this developmental transition. Endocytosis and autophagy, two pathways leading to the lysosomes, are highly upregulated during this period. A subset of maternal membrane proteins is selectively endocytosed and degraded in the lysosomes before the first mitotic cell division. UBC-13-dependent K63-linked ubiquitination is required for the proper sorting of membrane proteins. Autophagy is also induced shortly after fertilization and executes the degradation of paternally inherited embryonic organelles, e.g. mitochondria and membranous organelles. This mechanism underlies the maternal inheritance of the mitochondrial genome. Our study revealed physiological roles of lysosomal pathways during early development.
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Free Research Field |
発生細胞生物学
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