Dynamics of polarisome and ROS generating complex during pathogenic development of fungi

2013 Fiscal Year Final Research Report

• Project/Area Number: 23688006
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Kobe University
• Principal Investigator: IKEDA Kenichi 神戸大学, (連合)農学研究科(研究院), 准教授 (40437504)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 糸状菌 / 病原性 / 侵入力 / 活性酸素 / ポラリソーム / NADPH oxidase

Research Abstract

This study was evaluated the functional mechanism of NADPH oxidase (Nox) during host penetration. In black spot disease, two Nox homologues, NoxA and NoxB are present and only the NoxB was involved in host penetration. Therefore, we evaluated the expression pattern and localization of Nox(s). The expression of Nox were induced during spore germination. NoxB protein was localized at the appressorium. The disruption of noxB resulted in disorder of actin accumulation at the appressorium. Moreover, NoxB was interacted with RacA. The disruption mutant of racA was affected multiple hyphal growth stages and lost pathogenicity.

Research Products

• [Journal Article] Durable and broad-spectrum disease protection measure against airborne phytopathogenic fungi by using the detachment action of gelatinolytic bacteria (2014)
  • Author(s): Kitagawa, H., Shimoi, S., Inoue, K., Park, P., and Ikeda, K
  • Journal Title: Biological Control Volume: 71 Pages: 1-6
  • DOI: 10.1016/j.biocontrol.2013.12.008
  • Peer Reviewed
• [Journal Article] Is the fungus Magnaporthe losing DNA methylation? (2013)
  • Author(s): Ikeda, K., Vu, B. V., Kadotani, N., Tanaka, M., Murata, T., Shiina, K., Chuma, I., Tosa, Y., and Nakayashiki H
  • Journal Title: Genetics Volume: 195 Pages: 845-855
  • DOI: 10.1534/genetics.113.155978
  • Peer Reviewed
• [Journal Article] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific toxin-producing fungus Alternaria alternata Japanese pear pathotype (2013)
  • Author(s): Morita Y, Hyon GS, Hosogi N, Miyata, N., Nakayashiki, H., Muranaka, Y., Inada, N., Park, P., and Ikeda, K
  • Journal Title: Molecular Plant Pathology Volume: 14 Pages: 365-378
  • DOI: 10.1111/mpp.12013
  • Peer Reviewed
• [Presentation] Attachment of airborne pathogens to their host; a potential target for disease control (2013)
  • Author(s): Ikeda, K., Kitagawa, H., Shimoi, S., Inoue, K., Osaki, Y., Nakayashiki, H., and Park, P
  • Organizer: 10^<th> International Congress of Plant Pathology
  • Place of Presentation: Beijing, China
  • Year and Date: 2013-08-28
• [Presentation] Autophagy-inducing condition during infection process of Magnaporthe oryzae (2013)
  • Author(s): Inoue, K., Nakayashiki, H., and Ikeda, K
  • Organizer: 10^<th> International Congress of Plant Pathology
  • Place of Presentation: Beijing, China
  • Year and Date: 2013-08-26
• [Presentation] Macroautophagy during appressorium morphogenesis in Magnaporthe oryzae (2013)
  • Author(s): Inoue, K., Ikeda, K., and Nakayashiki, H
  • Organizer: 6^<th> International Rice Blast Conference 2013
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2013-08-22
• [Presentation] ナシ黒斑病菌のRacAの病原性及び胞子発芽に及ぼす影響 (2013)
  • Author(s): 森田雄一、玄康洙、伊藤里紗、中屋敷均、朴杓允、池田健一
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学、岐阜市
  • Year and Date: 2013-03-28
• [Presentation] Appressorium-localized NADPH oxidase is essential for aggressiveness and pathogenicity in Alternaria alternata. 2012 KSPP (The Korean Society of Plant Pathology) (2012)
  • Author(s): Ikeda, K., Morita, Y., Hyon, G.-S., and Park, P
  • Organizer: International Conference
  • Place of Presentation: Auditorium, Seoul National University, Seoul, Korea
  • Year and Date: 2012-10-26
• [Presentation] ナシ黒斑病菌の付着器局在性NADPH oxidase Bは侵略力発揮に必須である (2012)
  • Author(s): 森田雄一、玄康洙、中屋敷均、稲田のりこ、朴杓允、池田健一
  • Organizer: 平成24年度日本植物病理学会関西部会
  • Place of Presentation: 鳥銀文化会館、鳥取市
  • Year and Date: 2012-09-28
• [Presentation] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in host specific toxin producing fungus Alternaria alternata Japanese pear pathotype. (2012)
  • Author(s): Morita, Y., Hyon, G.-S., Hosogi, N., Morikawa, K., Kusaka, M., Nakayashiki, H., Inada, N., Tsuge, T., Park, P., and Ikeda, K
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto International Congress Center, Kyoto
  • Year and Date: 2012-07-31
• [Presentation] ゼラチナーゼ活性を有する微生物の葉面定着性の向上 (2012)
  • Author(s): 北川博子、朴杓允、池田健一
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場、福岡市
  • Year and Date: 2012-03-30
• [Presentation] Functional analyses of membrane binding NADPH oxidase complex, reactive oxygen species generation machinery, in phytopathogenic fungus Alternaria alternata Japanese pear pathotype (2012)
  • Author(s): Morita Y, Hyon GS, Park P, Ikeda K
  • Organizer: 日韓植物病理学合同シンポジウム
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-03-27
• [Presentation] 病原菌のアキレス腱をターゲットとした病害防除戦略 (2011)
  • Author(s): 池田健一
  • Organizer: 近畿作物・育種研究会公開シンポジウム
  • Place of Presentation: 神戸大学
  • Year and Date: 2011-12-03
• [Presentation] ナシ黒斑病菌の感染器官における活性酸素種生成複合体の機能解析 (2011)
  • Author(s): 森田雄一、玄康洙、森川響子、池田健一、朴杓允
  • Organizer: 糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京大学
  • Year and Date: 2011-11-17
• [Presentation] ナシ黒斑病菌の感染器官における活性酸素種生成複合体の機能解析 (2011)
  • Author(s): 森田雄一、玄康洙、森川響子、池田健一、朴杓允
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松
  • Year and Date: 2011-10-02
• [Presentation] ナシ黒斑病菌の付着器における活性酸素種生成の特性解析 (2011)
  • Author(s): 森田雄一、玄康洙、池田健一、朴杓允
  • Organizer: 医学生物学電子顕微鏡技術学会
  • Place of Presentation: 四国大学
  • Year and Date: 2011-05-15
• [Book] "The role of the extracellular matrix (ECM) in phytopathogenic fungi : a potential target for disease control"in Plant Pathology (2012)
  • Author(s): Ikeda K, Inoue K, Kitagawa H, Meguro H, Shimoi S, Park P
  • Total Pages: 131-150
  • Publisher: INTECH Open Access Publisher(DOI : 10.5772/30801)