Study on the molecular basis underlyng neurite outgrowth promotion by gangliosides

2014 Fiscal Year Final Research Report

• Project/Area Number: 23688014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Gifu University
• Principal Investigator: ANDO Hiromune 岐阜大学, 応用生物科学部, 准教授 (20372518)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Kenichi 京都大学, 物質-細胞統合システム拠点, 准教授 (50423059)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: ガングリオシド / 神経突起伸展 / 構造活性相関 / 一分子観察

Outline of Final Research Achievements

The goal of this research is to clarify the molecular basis underlyning the neurite outgrowth promotion by exogenous gangliosides. We have succeeded in the first total syntheses of neuritogenic gangliosides and their analogues based on the newly developed synthetic method. The synthesized gangliosides were utilized for neurite outgrowth evaluation, by which the structure responsible for the activity was revealed for the first time. Furthermore, we have also developed novel fluorescently labeled ganglioside probes suitable for observing the behavior of ganglioside in living cell membrane. The single molecular observation using the ganglioside probes allowed to observe how ganglioside molecule moves in the cell membrane (done by collaboration with Prof. Kenichi Suzuki of Kyoto University).

Free Research Field

糖鎖合成化学、生物有機化学