2014 Fiscal Year Final Research Report
Development of an efficient gene expression vector utilizing RNA-binding proteins and antisense transcripts
| Project/Area Number |
23689010
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
SAKURAI FUMINORI 大阪大学, 薬学研究科(研究院), 准教授 (70370939)
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| Research Collaborator |
MIZUGUCHI Hiroyuki 大阪大学, 大学院薬学研究科, 教授
TACHIBANA Masashi 大阪大学, 大学院薬学研究科, 助教
NAKAMURA Shin-ichiro 滋賀医科大学, 動物生命科学センター, 准教授
FUJIWARA Toshiyoshi 岡山大学, 医歯薬学総合研究科, 教授
SHIMIZU Kahori 大阪大谷大学, 薬学部, 助教
OKAMOTO Sayuri 大阪大学, 大学院薬学研究科, 非常勤職員
HOSOYAMADA Eri 大阪大学, 大学院薬学研究科, 非常勤職員
MACHITANI Mitsuhiro 大阪大学, 大学院薬学研究科
HAYASHI Kohei 大阪大学, 大学院薬学研究科
BENNETT David Mark Gilfedder 大阪大学, 大学院薬学研究科
IIZUKA Syunsuke 大阪大学, 大学院薬学研究科
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Keywords | gene therapy / non-coding RNA / microRNA / adenovirus vector / gene expression |
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| Outline of Final Research Achievements |
A novel gene expression vector which efficiently mediates transgene expression in a targeted organ-specific manner is required for achievement of safe and effective gene therapy.In this study, we tried to improve transgene expression profiles and therapeutic effects by regulating stability of mRNA. Dramatic improvement of transgene expression profiles was not found by utilizing HuR or AU-rich sequences. On the other hand, adenovirus (Ad) vector-mediated transgene expression profike was significantly improved by suppression of leaky expression of Ad genes utilizing non-coding RNA. This novel Ad vector also meidated efficient transduction in neonatal mice.
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| Free Research Field |
遺伝子治療学
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