Challenge in the assessment of the injured donor lungs and its treatment using an ex vivo lung perfusion

2013 Fiscal Year Final Research Report

• Project/Area Number: 23689063
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: FENGSHI Chen 京都大学, 医学(系)研究科(研究院), 助教 (00452334)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 肺移植 / 体外肺潅流 / 傷害肺 / 蘇生

Research Abstract

To encounter the donor shortage in lung transplantation, it is necessary to utilize the injured donor lungs, such as marginal donor lungs. It is essential to assess the quality of the injured donor lungs for using such injured lungs safely. Thus, we performed the study in which we assess the function of the donor lungs preoperatively using an ex vivo lung perfusion (EVLP) and even treat the injured lungs during EVLP. Firstly, we demonstrated that we could assess the function of the donor lungs accurately using EVLP. Secondly, the group with a drug inhalation showed a better lung function than the group without a drug inhalation, suggesting the possibility of treatment of the injured donor lungs during EVLP.

Research Products

• [Journal Article] Protective effect of surfactant inhalation against warm ischemic injury in an isolated rat lung ventilation model (2013)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Kobayashi M, Bando T, Date H
  • Journal Title: PLoS One Volume: 8(8) Pages: e72574
  • DOI: 10.1371/journal.pone.0072574
• [Journal Article] Protective effect of plasmin in marginal donor lungs in an ex vivo lung perfusion model (2013)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 32(5) Pages: 505-10
  • DOI: 10.1016/j.healun.2013.02.007
• [Journal Article] Reconditioning lungs donated after cardiac death using short-term hypothermic machine perfusion (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: Transplantation Volume: 94(10) Pages: 999-1004
  • DOI: 10.1097/TP.0b013e31826f632e
• [Journal Article] Therapeutic Effect of Surfactant Inhalation during Warm Ischemia in an Isolated Rat Lung Perfusion Model (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Yamada T, Fujinaga T, Shoji T, Sakai H,Bando T, Date H
  • Journal Title: Transpl Int Volume: 25(10) Pages: 1096-105
  • DOI: 10.1111/j.1432-2277.2012.01532.x
• [Journal Article] The effect of beta-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Zhao X, Sakai H, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31(7) Pages: 773-9
• [Journal Article] Reconditioning of The Lungs from Non-Heart-Beating Donors with Normothermic Ex Vivo Lung Perfusion (2012)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Ohsumi A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31(2) Pages: 187-93
• [Journal Article] Effect of pre-procurement ventilation on lungs donated after cardiac death in a canine lung transplantation model (2011)
  • Author(s): Sakamoto J, Chen F, Yamada T, Nakajima D, Ohsumi A, Kikuchi R, Zhao X, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 864-70
• [Journal Article] Hypothermic machine perfusion ameliorates ischemia-reperfusion injury in rat lungs from non-heart-beating donors (2011)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Osumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 858-63
• [Journal Article] Comparison of Extracellular-Type-Kyoto solution and Perfadex as a preservation solution in a pig ex vivo lung perfusion model : Impact of potassium level (2011)
  • Author(s): OkamotoT, Chen F, Zhang J, Choi H, Yamada T, Morikawa H, Nakayama E, Bando T, Date H
  • Journal Title: Transplant Proc Volume: 43(5) Pages: 1525-8
• [Journal Article] Normothermic ex vivo lung perfusion in clinical lung transplantation (2011)
  • Author(s): Cypel M, Yeung JC, Liu M, Anraku M, Chen F, Karolak W, Sato M, Laratta J, Azad S, Madonik M, Chow CW, Chaparro C, Hutcheon M, Singer LG, Slutsky AS, Yasufuku K, De Perrot M, Pierre AF, Waddell TK, Keshavjee S
  • Journal Title: N Eng J Med Volume: 364 Pages: 1431-40
• [Presentation] Correlation of physiological data at ex vivo lung perfusion and reperfusion in a rat ischemia-reperfusion model using plasmin (2014)
  • Author(s): Motoyama H, Chen F, Hijiya K, Takahashi M, Ohata K, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: Abstract#692, 34th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Diego
  • Year and Date: 20140410-13
• [Presentation] Length of the agonal phase in donors after cardiac death influences donor lung function after reperfusion in a rat model (2014)
  • Author(s): H ijiya K, Chen F, K. Takahashi M, Ohata K, Kondo T, Motoyoma H, Ohsumi A, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: Abstract#246, 34th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Diego
  • Year and Date: 20140410-13
• [Presentation] Protective effect of beta-2 adrenoreceptor agonist inhalation during ex vivo lung perfusion (2014)
  • Author(s): Kondo T, Chen F, K. Takahashi M, Ohata K, Motoyoma H, Hijiya K, Ohsumi A, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: Abstract#114, 34th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Diego
  • Year and Date: 20140410-13
• [Presentation] Ex Vivo Lung Perfusion (EVLP)における潅流液の違いについての検討 (2014)
  • Author(s): 本山秀樹、陳豊史、高橋守、大畑恵資、近藤健、土屋恭子、山田徹、毛受暁史、佐藤雅昭、青山晃博、佐藤寿彦、園部誠、大政貢、板東徹、伊達洋至
  • Organizer: 日本外科学会第114回定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 20140403-05
• [Presentation] PS1-199. Protective effect of beta-2 adrenorecptor agonist inhalation during ex vivo lung perfusion (2013)
  • Author(s): Kondo T, Chen F, Ohsumi A, Hijiya K, Motoyama H, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: The 13^<th> Congress of the Asian Society of Transplantation
  • Place of Presentation: Kyoto
  • Year and Date: 20130902-06
• [Presentation] Protective Effect of Plasmin in Marginal Donor Lungs in an Ex Vivo Lung Perfusion Model. Abstract#680 (2013)
  • Author(s): H. Motoyama, F. Chen, A. Ohsumi, K. Hijiya, K. Okita, K. Kondo, D. Nakajima, J. Sakamoto, T. Yamada, A. Aoyama, M. Sato, T. Bando, H. Date
  • Organizer: 33th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Montreal
  • Year and Date: 20130424-27
• [Presentation] 肺移植におけるトランスレーショナルリサーチPD-15-3 (2013)
  • Author(s): 陳豊史、大角明宏、本山秀樹、土屋恭子、尾北賢治、近藤健、中島大輔、阪本仁、山田徹、佐藤雅昭、青山晃博、板東徹、伊達洋至
  • Organizer: 日本外科学会第113回定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20130411-13
• [Presentation] Ex vivo lung perfusion施行中におけるβ-2受容体アゴニスト吸入の保護効果 (2013)
  • Author(s): 近藤健、陳豊史、大角明宏、土屋恭子、山田徹、佐藤雅昭、青山晃博、園部誠、大政貢、板東徹、伊達洋至
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2013-09-07
• [Presentation] 肺移植における体外肺潅流の歩み (2013)
  • Author(s): 陳豊史、大角明宏、本山秀樹、土屋恭子、近藤健、中島大輔、阪本仁、山田徹、佐藤雅昭、青山晃博、板東徹、伊達洋至
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2013-09-07
• [Presentation] Reconditioning of lungs donated after cardiac deathwith short-term hypothermic machine perfusion (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: CO10.02 XXIV International Congress of The Transplantation Society Berlin
  • Place of Presentation: Germany
  • Year and Date: 20120716-19
• [Presentation] Protective effect of plasmin for the non-heparinized donor lungs after cardiac death (2012)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: PO10.36 XXIV International Congress of The Transplantation Society Berlin
  • Place of Presentation: Germany
  • Year and Date: 20120716-19
• [Presentation] Prophylactic Surfactant Inhalation Attenuates Warm Ischemic Injury in Rat Lungs from Donation after Cardiac Death Donors (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Kobayashi M, Yamada T, Fujinaga T, Shoji T,Sakai H, Bando T, Date H
  • Organizer: CO33.08 XXIV International Congress of The Transplantation Society Berlin
  • Place of Presentation: Germany
  • Year and Date: 20120716-19
• [Presentation] 肺移植におけるサーファクタント療法 (2012)
  • Author(s): 大角明宏、陳豊史、阪本仁、中島大輔、土屋恭子、本山秀樹、尾北賢治、山田徹、佐藤雅昭、青山晃博、板東徹、伊達洋至
  • Organizer: 第48回日本肺サーファクタント・界面医学会シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2012-10-27
• [Presentation] 本邦での肺移植を安全に施行し普及させるための工夫 (2012)
  • Author(s): 陳豊史
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田
  • Year and Date: 2012-05-18
• [Presentation] イヌ心停止ドナー肺移植モデルにおけるサーファクタンと吸入の肺保護効果 (2012)
  • Author(s): 大角明宏、陳豊史、阪本仁、中島大輔、土屋恭子、本山秀樹、尾北賢治、菊地柳太郎、堀田健太、山田徹、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田
  • Year and Date: 2012-05-17
• [Presentation] Beta2-ADRENORECEPTOR AGONIST INHALATION AMELIORATES ISCHEMIA REPERFUSION INJURY FOLLOWING LUNG TRANSPLANTATION FROM NON-HEART-BEATING DONORS IN A CANINE MODEL (2011)
  • Author(s): Jin Sakamoto, Fengshi Chen, Daisuke Nakajima, Tetsu Yamada, Akihiro Ohsumi, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Toru Bando, Hiroshi Date
  • Organizer: The 12th Congress of the Asian Society of Transplantation
  • Place of Presentation: Seoul
  • Year and Date: 20110926-28
• [Presentation] Prophylactic surfactant inhalation ameliorates ischemia-reperfusion injury in rat lungs from donation after cardiac death donors (2011)
  • Author(s): Akihiro Ohsumi, Fengshi Chen, Daisuke Nakajima, Jin Sakamoto, Tetsu Yamada, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Toru Bando, Hiroshi Date
  • Organizer: The 12th Congress of the Asian Society of Transplantation
  • Place of Presentation: Seoul
  • Year and Date: 20110926-28
• [Presentation] 本邦における肺移植の現況と今後の展望シンポジウム3移植医療の新しい局面をむかえて-各臓器の変遷と今後の展望- (2011)
  • Author(s): 陳豊史
  • Organizer: 第36回日本外科系連合学会
  • Place of Presentation: 東京
  • Year and Date: 20110000
• [Presentation] 常温肺体外灌流一normothermic ex-vo lung perfusion(EVLP)一による心停止ドナー肺の回復 (2011)
  • Author(s): 中島大輔、陳豊史、山田徹、阪本仁、大角明宏、藤永卓司、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第28回日本呼吸器外科学会総会
  • Place of Presentation: 大分
  • Year and Date: 20110000
• [Book] 肺移植。呼吸。東京。(Vol 31, No. 9) (2012)
  • Author(s): 陳豊史、伊達洋至
  • Total Pages: 864-867
  • Publisher: レスピレーション・リサーチ・ファンデーション