Searching way for direct conversion of mesenchymal cells to keratinocytes

2013 Fiscal Year Final Research Report

• Project/Area Number: 23689073
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Kyorin University
• Principal Investigator: KURITA Masakazu 杏林大学, 医学部, 助教 (20424111)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 直接転換 / ケラチノサイト / 線維芽細胞 / 創傷治癒 / リプログラミング / 遺伝子導入 / 間葉上皮移行 / 上皮化

Research Abstract

In order to develop new therapeutic strategies for cutaneous ulcers, way for direct conversion of fibroblasts to keratinocytes are searched. Direct transdifferentiation to keratinocytes may enable de novo epithelialization from bottom of ulcers. Using microarrays and bioinformatic analysises, candidates factors for direct conversion to keratinocytes are selected. We picked up 55 transcription factors and 30 micro RNAs as candidates. Using lentiviral vectors, these factors are transduced to primary fibroblasts and obtained cells similar to keratinocytes in its form and proliferation kinetics.

Research Products

• [Presentation] ケラチノサイトに特異的なmicro RNAの同定 (2014)
  • Author(s): 栗田昌和、江藤ひとみ、多久嶋亮彦、波利井清紀
  • Organizer: 第21回日本形成外科学会基礎学術集会
  • Place of Presentation: ホテルリステル猪苗代、福島県
  • Year and Date: 20141004-05
• [Presentation] Searching the way of direct conversion of fibroblasts into keratinocytes for the treatment of cutaneous ulcers (2014)
  • Author(s): Kurita M, Eto H, Suga H, Takushima A, Harii K
  • Organizer: Cira International Symposium 2014
  • Place of Presentation: Center for Learning and Innovation (CLI), Takeda Pharmaceutical Company, Ltd., Osaka, Japan
  • Year and Date: 20140116-18
• [Presentation] 皮膚潰瘍上皮化促進を目的とした線維芽細胞からケラチノサイトへの直接転換のこころみ (2013)
  • Author(s): 栗田昌和、江藤ひとみ、佐藤卓志、菅浩隆、多久嶋亮彦、波利井清紀
  • Organizer: 第22回日本形成外科学会基礎学術集会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター、新潟県
  • Year and Date: 20131107-08
• [Presentation] 皮膚潰瘍上皮化促進を目的とした線維芽細胞からケラチノサイトへの直接転換のこころみパネルディスカッション1創傷治癒の分子メカニズム (2013)
  • Author(s): 栗田昌和、江藤ひとみ、菅浩隆、多久嶋亮彦、波利井清紀
  • Organizer: 第5回日本創傷外科学会総会・学術集会
  • Place of Presentation: ホテルグランヴィア京都、京都府
  • Year and Date: 20130711-12
• [Presentation] Searching the way of direct conversion of fibroblasts into keratinocytes for the treatment of cutaneous ulcers (2013)
  • Author(s): Kurita M Eto H, Suga H, Takushima A, Harii K
  • Organizer: Cira International Symposium 2013
  • Place of Presentation: Kyoto University, Clock tower centennial hall, Kyoto, Japan
  • Year and Date: 20130511-12