2013 Fiscal Year Final Research Report
Identification of novel transcription factors involved in neuronal maintenance and degeneration.
| Project/Area Number |
23700430
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YAMANAKA TOMOYUKI 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (00381575)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経変性 / ハンチントン病 / 転写因子 / 遺伝子発現 |
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| Research Abstract |
Through screening using Panomics Protein-DNA array, we identified several transcription factors (TFs) whose DNA binding activities were affected in Huntington's disease (HD) model mice brain. One of them, an E-box binding factor, interacted with mutant huntingtin aggregates. We identified ~300 downstream genes of it by ChIP-chip, and some of their expressions were affected in HD model brains. These data suggest the suppression of E-box binding factor by mutant huntingtin may be involved in some aspects of HD pathogenesis. We also examined NF-Y, another TF affected by mutant huntingtin, by analyzing its neuronal knockout mice or ChIP-chip data, and revealed its critical role in neuronal cell maintenance and degeneration.
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