2012 Fiscal Year Final Research Report
Development of conditional expression system in mice generated by site-specific gene integration method
| Project/Area Number |
23700514
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | トランスジェニックマウス / 顕微注入法 / Cre-loxP / PITT / コンディショナル発現 |
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| Research Abstract |
The aim of this study is to make our targeted transgenesis method (PITT) more versatile by developing a conditional and tissue-specific expression system applicable to it. For this purpose, we conducted: 1) generation of PITT mice exhibiting tissue-specific transgene expression, 2) development of Dre reporter mouse useful for evaluation of Dre expression, 3) development of new seed mouse line that allows to integrate floxed allele, and 4) development of inducible expression system using KRAB repressor.
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[Book] Human Diseases and Evolution. Chapter 132011
Author(s)
OhtsukaM., TakedaH., and Shimada A
Total Pages
201-215
Publisher
Double anal fin (Da): a medaka mutant exhibiting a mirror-image pattern duplication of the dorsal-ventral axis. in Medaka: Model for Organogesi
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