2013 Fiscal Year Final Research Report

Protein carrier nanomachine

Research Project

Project/Area Number	23700539
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biomedical engineering/Biological material science
Research Institution	Kyushu University
Principal Investigator	MORI Takeshi 九州大学, 工学(系)研究科(研究院), 准教授 (70335785)
Project Period (FY)	2011 – 2013
Keywords	タンパク質 / ナノメディスン / 細胞膜 / エンドサイトーシス / 免疫治療
Research Abstract	Here, we synthesized dextrans modified with trivalent cationic or anionic groups. Aqueous solutions of the cationic and anionic dextrans were then mixed resulting in the formation of polyion complex nanogels (PIC-NGs), which have physically crosslinked salt bridges formed between the cationic and anionic groups. We have designed biotinylated polymers as synthetic receptors that have multiple alkyl groups for endocytotic delivery of target proteins. The polymers were stably attached to a cell surface via multivalent anchoring. The presented biotin was bound to streptavidin (SA) on the cell surface, and, via an endocytotic pathway, the cell rapidly internalized the biotinylated polymer/SA complex.