Investigation of nucleic acid type ligand which can use in vivo

2012 Fiscal Year Final Research Report

• Project/Area Number: 23700552
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Hokkaido University
• Principal Investigator: HYODO Mamoru 北海道大学, 大学院・薬学研究院, 特任助教 (30548186)
• Project Period (FY): 2011 – 2012
• Keywords: バイオコンジュゲート材料

Research Abstract

We have isolated the DNA aptamer named AraHHOOI from 12rounds of selection to tumor endothelial cells and counter selection with normal endothelialcells and tumor parenchymal cells. AraHHOOl aptamer showed 44 nM dissociationconstant to tumor endothelial cells and incorporated into tumor endothelial cells.AraHHOOI aptamer binds to Troponin T protein on tumor endothelial cells and AraHHOOlaptamer bound liposome showed 3.5 times higher uptake efficacy compared to normalliposome.

Research Products

• [Journal Article] Development of a novel DNAaptamer ligand targeting to primary culturedtumor endothelial cells by a cell-based SELEXmethod (2012)
  • Author(s): Mst. Naznin Ara, Mamoru Hyodo,Noritaka Ohga, Kyoko Hida, Hideyoshi Harashima
  • Journal Title: PLoS One Volume: 7 Pages: e50174
  • DOI: doi:10.1371fjoumal.pone.0050174
  • Peer Reviewed
• [Journal Article] A pH-sensitive cationiclipid facilitates the delivery of liposomalsiRNA and gene silencing activity in vitro andin vivo.ノ. Control (2012)
  • Author(s): Yusuke Sato, Hiroto Hatakeyama, YuSakurai, Mamoru Hvodo, Hidetaka Akita,Hideyoshi Harashima
  • Journal Title: Release Volume: 163 Pages: 267-276
  • DOI: doi:10.1016/j.jconrel.2012.09.009
  • Peer Reviewed
• [Journal Article] Vascular-targetednanotherapy for obesity:Unexpected passivetargeting mechanism to obese fat for theenhancement of active drug delivery.ノ.Control (2012)
  • Author(s): Md. Nazir Hossen, Kazuaki Ksjimoto,Hidetaka Akita, Mamoru Hyodo and Hideyoshi Harashima
  • Journal Title: Release Volume: 163 Pages: 101-ll0
  • DOI: doi:10.1016/j.jconrel.2012.09.002
  • Peer Reviewed
• [Journal Article] BreakerIdentification of ligand Analogues that controlc-di-GMP Riboswitches (2012)
  • Author(s): Kazuhiko Furukawa, Hongzhou Gu,Narasimhan Sudarsan, Yoshihiro Hayakawa,Mamoru Hvodo and Ronald R
  • Journal Title: ACS Chem. Biol Volume: 7 Pages: 1436-1443
  • DOI: doi:10.1021/cb300138n
  • Peer Reviewed
• [Presentation] Isolation ofnew DNA aptamers for pancreatic cancer cells.Abstract (2012)
  • Author(s): Mamoru Hvodo, Mst. Naznin Ara, YusukeTakaya and Hideyoshi Harashima
  • Organizer: IS3NA XX intemationalroundtable
  • Place of Presentation: Centre Mont-Royal, Montreal,Canada
  • Year and Date: 20120805-09
• [Presentation] Investigation of RNA aptamers targetingmitochondria by mitochondria based SELEX.Abstract No.149 (2012)
  • Author(s): Yuri Tawaraya, Mamoru Hyodo, YumaYamada and Hideyoshi Harashima
  • Organizer: IS3NA XX intemationalroundtable
  • Place of Presentation: Centre Mont-Royal Montreal,Canada
  • Year and Date: 20120805-09
• [Presentation] ミトコンドリア標的RNAアプタマーの探索 (2012)
  • Author(s): 俵谷優里、兵膣宣,、山田勇磨、原島秀吉
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 20120524-26
• [Presentation] Development of a new DNA aptamer againstprimary cultured tumor endothelial cells by acell-based SELEX method to discover a newdrug delivery system, Abstract No.3886 (2012)
  • Author(s): Mst Naznin Ara, Mamoru HvodΩ, NoritakaOhga, Kyoko Hida, Hideyoshi Harashima
  • Organizer: AACR annual meeting 2012
  • Place of Presentation: McCormickPlace, Chicago, Illinois, USA
  • Year and Date: 20120331-0404
• [Patent(Industrial Property Rights)] トロポニンTと結合するDNA、これを用いた腫瘍組織における血管新生および/または脈管形成抑制剤ならびにこれを用いた細胞内へ目的物質が取り込まれることを促進する剤 (2012)
  • Inventor(s): アラ ナズニン,兵藤宣,畠山浩人,樋田京子,大賀則孝,原島秀吉
  • Industrial Property Rights Holder: バイオメッドコア,アラ ナズニン,兵藤立,畠山浩人,樋田京子,大賀則孝,原島秀吉
  • Industrial Property Number: 特願,2012-124073
  • Filing Date: 2012-06-21