Development of protein nanocage for siRNA delivery

2012 Fiscal Year Final Research Report

• Project/Area Number: 23700561
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyushu University
• Principal Investigator: TABATA Shigekazu 九州大学, 薬学研究院, 学術研究員 (00538928)
• Project Period (FY): 2011 – 2012
• Keywords: 薬物伝達システム / siRNA / ナノバイオテクノロジー

Research Abstract

In this project, I tried preparing functionalized sHsp16.5 as a drug career. I fused sHsp16.5 with a tag which directed proteins to degradation in cells and some results indicated that the tag could work properly.

Research Products

• [Journal Article] Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages (2012)
  • Author(s): Masaharu Murata, Sayoko Narahara, Kaori Umezaki, Riki Toita, Shigekazu Tabata, Jing Shu Piao, Kana Abe, Jeong-Hun Kang, Kenoki Ohuchida, Lin Cui, Makoto Hashizume
  • Journal Title: International Journal of Nanomedicine Volume: 第7巻 Pages: 4353-4362
  • DOI: DOI:10.2147/IJN.S31365
  • Peer Reviewed
• [Journal Article] Development of Human Hepatocellular Carcinoma Cell-Targeted Protein Cages (2012)
  • Author(s): Riki Toita, Masaharu Murata, Shigekazu Tabata, Kana Abe, Sayoko Narahara, Jing Shu Piao, Jeong-Hun Kang, Makoto Hashizume
  • Journal Title: Bioconjugate Chemistry Volume: 第23巻 Pages: 1494-1501
  • DOI: DOI:10.1021/bc300015f
  • Peer Reviewed
• [Presentation] 高反応性ペプチドタグデザインに基づいたタンパク質特異的ラベル化法の開発 (2013)
  • Author(s): 田畑栄一、城戸宗継、多仁一司、浜地格、王子田彰夫
  • Organizer: 日本薬学会第133 年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 20130327-30
• [Presentation] 高反応性ペプチドタグデザインに基づいた タンパク質特異的ラベル化システムの開発(2) (2013)
  • Author(s): 田畑栄一、城戸宗継、浜地格、王子田彰夫
  • Organizer: 日本化学会第93 春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス(滋賀県)
  • Year and Date: 20130323-25
• [Presentation] 高反応性ペプチドタグデザインに基づいた タンパク質特異的ラベル化システムの開発 (2013)
  • Author(s): 城戸宗継、田畑栄一、浜地格、王子田彰夫
  • Organizer: 日本化学会第93 春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス(滋賀県)
  • Year and Date: 20130322-25
• [Presentation] Design of a Highly Reactive Peptide Tag-Probe Pair for Selective Covalent Labeling of Cell Proteins (2012)
  • Author(s): Shigekazu Tabata, Munetsugu Kido, Kazushi Tani, Itaru Hamachi,Akio Ojida
  • Organizer: The First International Symposium on Biofunctional Chemistry 2012 (ISBC2012)
  • Place of Presentation: 東京工業大学 東工大蔵前会館(東京都)
  • Year and Date: 20121128-30
• [Presentation] タグ・小分子プローブペアを用いたタンパク質の特異的ラベル化 (2012)
  • Author(s): 田畑栄一、城戸宗継、浜地格、王子田彰夫
  • Organizer: 第11回次世代を担う若手ファーマ・バイオフォーラム
  • Place of Presentation: 九州大学医系キャンパス(福岡県)
  • Year and Date: 20120915-16