Immunological monitoring technique for dynamic analysis of environmental allergens

2012 Fiscal Year Final Research Report

• Project/Area Number: 23710003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MIYAJIMA Kumiko 東京医科歯科大学, 生体材料工学研究所, 技術職員 (10516298)
• Research Collaborator: MITSUBAYASHI Kohji 東京医科歯科大学, 生体材料工学研究所, 教授 (40307236) ; KUDO Hiroyuki 東京医科歯科大学, 生体材料工学研究所, 講師 (70329118) ; ARAKAWA Takahiro 東京医科歯科大学, 生体材料工学研究所, 助教 (50409637)
• Project Period (FY): 2011 – 2012
• Keywords: 環境計測

Research Abstract

A fiber-optic immunoassay system based on chemifluorescence detection for Der f1 derived from Dermatophagoides farinae. In this system, a light emitting diode (LED) was used as an excitation light source, and a photomultiplier was used as a fluorescence detector. Capture antibodies were immobilized on the end face of the optical fiber probe, and immunocomplexes were formed and labeled with fluorophores. Calibration range of the system for Der f1 defined by the fluorescence intensities was7.8-250 ng/ml. The study proved that the immunoassay system was useful in the basic technology for the on-site monitoring of environmental allergens.

Research Products

• [Journal Article] Fiber-optic fluoroimmunoassay system with a flow-through cell for rapid on site determination of Escherichia coli O157:H7 by monitoring fluorescence dynamics (2013)
  • Author(s): Miyajima K, Koshida T, Arakawa T, Kudo H, Saito H, Yano K, Mitsubayashi K
  • Journal Title: Biosensors Volume: 3(1) Pages: 120-131
  • Peer Reviewed
• [Journal Article] Fiber-optic biochemical gas sensor (bio-sniffer) for sub-ppb monitoring of formaldehyde vapor (2012)
  • Author(s): Kudo H, Wang X, Suzuki Y, Ye M, Yamashita T, Gessei T, Miyajima K, Arakawa T, Mitsubayashi K
  • Journal Title: Sensors and Actuators B-Chemical Volume: 161(1) Pages: 486-492
  • Peer Reviewed
• [Journal Article] A highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer (2012)
  • Author(s): Arakawa T, Ando E, Wang X, Miyajima K, Kudo H, Saito H, Mitani T, Takahashi M, Mitsubayashi K
  • Journal Title: Luminescence Volume: 27 Pages: 328-333
  • Peer Reviewed
• [Journal Article] Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents (2011)
  • Author(s): Chu MX, Miyajima K, Takahashi D, Arakawa T, Sano K, Sawada S, Kudo H, Iwasaki Y, Akiyoshi K, Mochizuki M, Mitsubayashi K.
  • Journal Title: Biomedical Microdevices Volume: 13 Pages: 603-611
  • Peer Reviewed
• [Journal Article] Soft contact lens biosensor for in situ monitoring of tear glucose as non-invasive blood sugar assessment (2011)
  • Author(s): Chu MX, Miyajima K, Takahashi D, Arakawa T, Sano K, Sawada S, Kudo H, Iwasaki Y, Akiyoshi K, Mochizuki M, Mitsubayashi K.
  • Journal Title: Talanta Volume: 83 Pages: 960-965
  • Peer Reviewed
• [Journal Article] Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f 1) (2011)
  • Author(s): Miyajima K, Itabashi G, Koshida T, Tamari K, Takahashi D, Arakawa T, Kudo H, Saito H, Yano K, Shiba K, Mitsubayashi K.
  • Journal Title: Environmental Monitoring and Assessment Volume: 182 Pages: 233-41
  • Peer Reviewed
• [Presentation] Fiber-optic fluoroimmunoassay system for on site monitoring of house dust mite allergen, International Conference on BioElectronics, BioSensors, BioMedical Devices (2012)
  • Author(s): Miyajima K, Tamari K, Kiyomiya E, Arakawa T, Kudo H, Shiba K, Mitsubayashi K
  • Organizer: BioMEMS/NEMS and Applications 2012
  • Place of Presentation: Singapore
  • Year and Date: 20121100
• [Presentation] 住環境におけるアレルゲンの連続計測をめざした光ファイバ式蛍光免疫計測法に関する研究 (2012)
  • Author(s): 宮島久美子,玉利佳子,清宮枝理世,荒川貴博,工藤寛之,芝紀代子,三林浩二
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢
  • Year and Date: 20120900
• [Presentation] 環境アレルゲン動態評価のための蛍光免疫計測システムに関する研究 (2012)
  • Author(s): 宮島久美子,玉利佳子,清宮枝理世,荒川貴博,工藤寛之,芝紀代子,三林浩二
  • Organizer: 第59回応用物理学会連合講演会
  • Place of Presentation: 東京
  • Year and Date: 20120300
• [Presentation] Fiber-optic fluoroimmunoassay system for on site determination of the indoor allergen (2012)
  • Author(s): Miyajima K, Tamari K, Kiyomiya E, Hayashi M, Arakawa T, Kudo H, Shiba K, Mitsubayashi K
  • Organizer: Pacific Rim Meeting on Electrochemical and solid-state Science (PRiME2012)
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2012-10-11
• [Presentation] Fiber-optic fluoro-immunosensor for monitoring of indoor allergens (2011)
  • Author(s): Miyajima K, Tamari K, Kiyomiya E, Arakawa T, Kudo H, Shiba K, Mitsubayashi K.
  • Organizer: 9th Asian Conference on Chemical Sensors
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 20111100
• [Presentation] Fiber-optic fluoroimmunoassay system for determination of dust mite allergen to improve indoor environment (2011)
  • Author(s): Miyajima K, Tamari K, Arakawa T, Kudo H, Shiba K, Mitsubayashi K.
  • Organizer: 2nd International Conference on Bio-Sensing Technology2011
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 20111000
• [Presentation] 環境アレルゲン動態評価のための光ファイバ式蛍光免疫計測システムに関する研究 (2011)
  • Author(s): 宮島久美子,玉利佳子,清宮枝理世,荒川貴博,工藤寛之,芝紀代子,三林浩二.
  • Organizer: 電気学会研究会センサ・マイクロマシン部門総合研究会
  • Place of Presentation: 東京
  • Year and Date: 20110600