2012 Fiscal Year Final Research Report
Regulation of nuclear architecture of neuronal cells by nuclear matrix protein SP120
| Project/Area Number |
23710216
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Okayama University |
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Principal Investigator |
MIYAJI Mari 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50349255)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経細胞 / 核内構造 / AT-rich DNA / 核マトリクス |
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| Research Abstract |
Several neuron-specific genes placed in the AT-rich genomic environment are regulated by DNA topoisomerase (topo) IIβ. We have identified hnRNP U/SAF-A/SP120 as a protein associated with topo IIβ in differentiating cerebellar neurons. SP120 is a multifunctional nuclear protein that directly binds to DNA and RNA. SP120 specifically and cooperatively binds AT-rich matrix attachment regions (MAR). It was the reported that the N-terminal domain termed SAF-box was required for DNA binding and the C-terminal domain called RGG-box was essential for the interaction with RNA. We showed that a C-terminal domain enriched with Arg-Gly (RG domain) encompassing the RGG-box was important for the MAR-specific binding activity.
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