Development of a method to analyze metabolic dynamics based on in vitro metabolic pathway

2012 Fiscal Year Final Research Report

• Project/Area Number: 23710236
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Keio University
• Principal Investigator: SAITO Natsumi 慶應義塾大学, 政策・メディア研究科, 特任講師 (50287546)
• Project Period (FY): 2011 – 2012
• Keywords: メタボロミクス / 酵素エフェクター / アロステリック制御

Research Abstract

We developed a method to reveal metabolic mechanisms based on the in vitro functionalization of selected metabolic pathway in combination with metabolome analysis. The method was applied to analyze metabolic perturbation v ia feedback regulation and furth

Research Products

• [Journal Article] tRNA thiolation pathway modulates intracellular redox state in Escherichia coli (2013)
  • Author(s): Nakayashiki, T., N. Saito, R. Takeuchi, H. Kadokura, K. Nakahigashi, H. Mori.
  • Journal Title: J. Bacteriol. Volume: 195 Pages: 2039-49
• [Journal Article] Characterization of a novel lipolytic enzyme from Aspergillus oryzae. (2012)
  • Author(s): Koseki, T., S. Asai, N. Saito, M. Mori, Y. Sakaguchi, K. Ikeda, and Y. Shiono
  • Journal Title: Appl. Microbiol. Biotechnol
  • DOI: DOI:10.1007/s00253-012-4391-7
• [Journal Article] メタボロミクスを利用した酵素機能解析 (2011)
  • Author(s): 斎藤菜摘,ロベールマルタン
  • Journal Title: 生化学(ミニレビュー) Volume: 83 Pages: 1039-1043
• [Presentation] In vitro代謝経路による酵素エフェクター同定方法 (2013)
  • Author(s): 斎藤菜摘,曽我朋義, 冨田勝
  • Organizer: 日本農芸化学会 2013 年度大会
  • Place of Presentation: 仙台
  • Year and Date: 20130325-27
• [Presentation] 大腸菌をモデル生物とした細胞の代謝・増殖制御機構の解明 (2012)
  • Author(s): 島田友裕, 斎藤菜摘, 田中寛
  • Organizer: 第7回メタボロームシンポジウム
  • Place of Presentation: 鶴岡
  • Year and Date: 20121010-12
• [Presentation] 乳酸菌のキシロース代謝経路 (2012)
  • Author(s): 園元謙二, 善藤威史, 斎藤菜摘, 中東憲治, 門多真理子, 吉川博文
  • Organizer: 第7回メタボロームシンポジウム
  • Place of Presentation: 鶴岡
  • Year and Date: 20121010-12
• [Presentation] メタボロミクスによる微生物代謝メカニズム解明に向けたアプローチ (2012)
  • Author(s): 斎藤菜摘
  • Organizer: 日本ゲノム微生物学会第6回若手の会
  • Place of Presentation: 静岡
  • Year and Date: 20120927-28
• [Presentation] In vitro metabolic pathway reveals the action of enzyme effectors. (2012)
  • Author(s): Saito, N., M. Ohishi, S. Ota, T. Soga, and M. Tomita
  • Organizer: Metabolomics Society- 8th Annual International conference (Metabolomics 2012)
  • Place of Presentation: Washington, D. C., USA
  • Year and Date: 20120625-28
• [Presentation] 階層的な代謝応答から理解する大腸菌緊縮応答の代謝メカニズム (2012)
  • Author(s): 斎藤菜摘, 中東憲治, 増田豪, 曽我朋義, 冨田勝
  • Organizer: 日本農芸化学会 2012 年度大会
  • Place of Presentation: 京都
  • Year and Date: 20120323-25
• [Presentation] 希少放線菌 Actinoplanes missouriensis の運動性胞子におけるエネルギー代謝の解析 (2012)
  • Author(s): 乙供かな依, 斎藤菜摘, 曽我朋義, 藤田信之, 早川正幸, 大西康夫
  • Organizer: 日本農芸化学会 2012 年度大会
  • Place of Presentation: 京都
  • Year and Date: 20120323-25
• [Presentation] メタボロミクスによる微生物の代謝メカニズム解明 (2012)
  • Author(s): 斎藤菜摘, 中東憲治, 増田豪, 曽我朋義, 冨田勝
  • Organizer: 山形分子生物学セミナー
  • Place of Presentation: 鶴岡
  • Year and Date: 2012-11-26
• [Presentation] Hierarchical manner of metabolic response under the stringent response in Escherichia coli. (2011)
  • Author(s): Saito, N
  • Organizer: UK-Japan joint workshop on Microbial Systems Biology
  • Place of Presentation: Tsuruoka
  • Year and Date: 20111024-25
• [Presentation] Metabolic shifts under the stringent response in Escherichia coli (2011)
  • Author(s): Saito, N., K. Nakahigashi, T. Masuda, A. Hirayama, T. Soga, and M. Tomita
  • Organizer: Metabolomics Society- 7th Annual International conference
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20110627-30
• [Presentation] メタボロミクスによる酵素機能と代謝メカニズム解明へのアプローチ (2011)
  • Author(s): 斎藤菜摘
  • Organizer: 日本農芸化学会東北支部第12回若手の会
  • Place of Presentation: 鶴岡
  • Year and Date: 2011-10-07