2012 Fiscal Year Final Research Report
Discovery and characterization of ES/iPSCs-cardiac differentiation promoting molecules by using chemical screening system
| Project/Area Number |
23710269
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Kyoto University |
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Principal Investigator |
MINAMI Itsunari 京都大学, 物質-細胞統合システム拠点, 助教 (40362537)
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| Project Period (FY) |
2010 – 2012
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| Keywords | iPS細胞 / 心筋分化 / ケミカルスクリーニング / 低分子化合物 / 臨床グレード |
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| Research Abstract |
I discovered a novel small molecule (KY02111) by the chemical screening system using ES cells. This molecule promotes iPS-cardiac differentiation strongly and robustly. By using this molecule, I developed a new cardiac differentiation method that has many advantages for clinical application because of high efficiency, no xeno-contamination and low cost. In addition, this method can create mature cardiac cellsfrom iPSCs comparing with other studies. This study was published by Cell Reports (a sister journal of Cell) and selected for ‘Best of Cell Reports’ of 2012. Moreover, we made cardiac sheets by using my method, and for the first time, we established a heart disease model that is the spiral wave propagation in an arrhythmia after myocardial infarction.
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[Journal Article] A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions2012
Author(s)
Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Heuser TT, Heuser JE, Uesugi M, Aiba K, Nakatsuji N
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Peer Reviewed
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