西南諸島に産する両生類絶滅危惧種の遺伝的多様性の解明と飼育繁殖における遺伝的管理

2013 Fiscal Year Annual Research Report

• Project/Area Number: 23710282
• Research Institution: Hiroshima University
• Principal Investigator: 井川 武 広島大学, 理学(系)研究科(研究院), 特任助教 (00507197)
• Keywords: 集団遺伝学 / 保全遺伝学 / 景観遺伝学 / 両生類 / 絶滅危惧種 / マイクロサテライト / 西南諸島 / GIS

Research Abstract

絶滅危惧種の遺伝的多様性を維持することは、その生物種の進化史を後世に残し、さらに将来の環境変動に適応して存続するための進化的可塑性を与える唯一の方策である。本研究では、遺伝的多様性を考慮した持続的な保全と、飼育下繁殖の遺伝的な環境を整備することを目的として、西南諸島に固有な絶滅危惧両生類6種について、マイクロサテライトマーカーの開発と、それらを用いた集団遺伝学的解析を行った。最終年度においては、ハナサキガエル種群におけるマイクロサテライトマーカーの開発を行うとともに、ハナサキガエルの集団構造解析を行い、さらにアマミハナサキガエルにおいて、発生段階における近親交配と温度上昇の影響を検証した。これらの結果は、1報が投稿中、2報が投稿準備中である。また、イボイモリについては、すべての島嶼集団における飼育下繁殖に成功し、国際誌に掲載された。
これにより、本研究の研究期間全体を通じて、最終的に対象種すべてに対してマイクロサテライトマーカーの開発を完了するとともに、集団構造解析を行うことができた。その結果として、島嶼内にも明確な遺伝的集団構造が存在し、それらは進化的背景と、それぞれの種の種生態とそれに関連した景観要因を反映して、独自のパターンを形成していた。これらの結果は、本邦産両生類において詳細な集団構造を解明した初めての研究成果であるとともに、当該種の保全活動への基礎的な情報として重要な知見となり得る。

Research Products

• [Journal Article] Genetic divergences of South and Southeast Asian frogs : a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya. (2014)
  • Author(s): Mahmudul Hasan, Mohammed Mafizul Islam, Mukhlesur Rahman Khan, and Takeshi Igawa, Mohammad Shafiqul Alam, HonTjong Djong, Nia Kurniawan, Hareesh Joshy, Yong Hoi Sen, Daicus M. Belabut, Atsushi Kurabayashi, Mitsuru Kuramoto, Masayuki Sumida
  • Journal Title: Turkish Journal of Zoology Volume: inpress Pages: -
  • Peer Reviewed
• [Journal Article] Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae). (2014)
  • Author(s): Shohei Komaki, Takeshi Igawa*, Masafumi Nozawa, Si-Min Lin, Shohei Oumi, and Masayuki Sumida
  • Journal Title: Genes & Genetic Systems Volume: inpress Pages: -
  • Peer Reviewed
• [Journal Article] Population structure and landscape genetics of two endangered frog species of genus Odorrana : different scenarios on two islands (2013)
  • Author(s): Takeshi Igawa, Shohei Oumi, Sseiki Katsuren, and Masayuki Sumida
  • Journal Title: Heredity Volume: 110 Pages: 46-56
  • DOI: 10.1038/hdy.2012.59
  • Peer Reviewed
• [Journal Article] Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan. (2013)
  • Author(s): Takeshi Igawa, Hirotaka Sugawara, Miyuki Tado, Takuma Nishitani, Atsushi Kurabayashi, Mohammed Islam, Shohei Oumi, Seiki Katsuren, Tamotsu Fujii, Masayuki Sumida
  • Journal Title: Animals Volume: 3 Pages: 680-692
  • DOI: 10.3390/ani3030680
  • Peer Reviewed
• [Journal Article] Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti). (2013)
  • Author(s): Ryosuke Kakehashi, Takeshi Igawa, Noriko Iwai, Etsuko Shoda-Kagaya, and Masayuki Sumida
  • Journal Title: Conservation Genetics Resources Volume: 5 Pages: 1071-1073
  • DOI: 10.1007/s12686-013-9981-x
  • Peer Reviewed
• [Journal Article] Stable aquaculture of the Japanese lancelet Branchiostoma japonicum for 7 years. (2013)
  • Author(s): Kinya Yasui, Takeshi Igawa, Takao Kaji, and Yasuhisa Henmi
  • Journal Title: Journal of experimental zoology Volume: 320B Pages: 538-547
  • DOI: 10.1002/jez.b.22540
  • Peer Reviewed
• [Presentation] Conservation genetics of endangered amphibians in Ryukyu Archipelago towards sustainable conservation of the biodiversity hotspot of amphibians (2014)
  • Author(s): Takeshi Igawa
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
  • Invited
• [Presentation] An ex situ conservation effort for several endangered and near-threaten amphibian species from the Ryukyu Archipelago, Japan: Captive breeding of nine anuran species and cryopreservation of sperm for two of them (2014)
  • Author(s): Islam, MM., Igawa, T., Kurabayashi, A., Kakehashi, R., Satou, N., Shintani, N., Tado, M., Sugawara, H., Nishitani, T., Uchida, M., Oumi, S., Katsuren, S., Fujii, T., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
  • Invited
• [Presentation] A case study of fauna conservation in Japan: Current conservation measures of wild populations, captive breeding, genetic diversity, and potential resource materials in the endangered frog species Odorrana ishikawae and O. splendida. (2014)
  • Author(s): Sumida, M., Islam, MM., Igawa, T., Kurabayashi, A., Satou, N., Ukena, K., Oumi, S., Katsuren, S., and Fujii, T
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
  • Invited
• [Presentation] Translucent frogs created through crossbreeding, and their inheritance and dermal chromatophore structure. (2014)
  • Author(s): Sumida, M., Islam, MM., Igawa, T., Kurabayashi, A., Furukawa, Y., Sano, N., Fujii, T., Yoshizaki, N., and *Nishioka, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] International Xenopus Resource Network and Educational Activities at Institute for Amphibian Biology. (2014)
  • Author(s): Suzuki, A., Kashiwagi, A., Kashiwagi, K., Hanada, H., Takebayashi-Suzuki, K., Tazawa, I., Furuno, N., Kurabayashi, A., Kobayashi, S., Takenaka, J., Tamaki, Y., Igawa, T., Uto, T., Nanba, C., Watanabe, A., Yoshida, H., Shimada, A., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] Inbreeding coefficient and genetic relationship of seven strains of Xenopus tropicalis inferred from genome wide genotyping of 54 microsatellite loci (2014)
  • Author(s): Watanabe, A., Igawa, T., Kashiwagi, A., Suzuki, A., Kurabayashi, A., Fujii, T., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in X. laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2014)
  • Author(s): Uno, Y., Nishida, C., Takagi, C., Igawa, T., Ueno, N., Sumida, M., and Matsuda, Y.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] Eco-evo-devo study of phenotypic plasticity in the Hokkaido salamander (Hynobius retardatus). (2014)
  • Author(s): Matsunami, M., Kishida, O., Kitano, J., Nozawa, M., Igawa, T., Michimae, H., Miura, T., and Nishimura, K.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] Genetic population structure of an endangered frog species, Babina holsti. (2014)
  • Author(s): Kakehashi, R., Igawa, T., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] New insights into evolutionary history among Asian pond frog species. (2014)
  • Author(s): Komaki, S., Igawa, T., Tojo, K., Kurabayashi, A., Lin, S., Min, M., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] Development and characterization of 27 new microsatellite markers of Indian Bullfrog, Hoplobatrachus tigerinus (Daudin, 1802) and its congeneric species. (2014)
  • Author(s): Sultana, N., Igawa, T., Nozawa, M., Islam MM., Khan, MR., Hasan, M., Alam, MS., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] Evolutionary relationships and postmating isolation among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by mtDNA Cyt b gene sequences and crossing experiments. (2014)
  • Author(s): Hasan, M., Kurniawan, N., Soewondo, A., Islam, MM., Igawa, T., Nalley, WMM., Matsui, M., and Sumida, M.
  • Organizer: International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing"
  • Place of Presentation: 東広島市
  • Year and Date: 20140327-20140328
• [Presentation] 絶滅危惧種ホルストガエルにおける遺伝的集団構造 (2013)
  • Author(s): 掛橋竜祐・井川武・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
  • Year and Date: 20131102-20131103
• [Presentation] Ion Torrent PGMを用いたハナサキガエルにおけるマイクロサテライト遺伝子座の単離と集団構造解析 (2013)
  • Author(s): 井川武・長岡麻衣・野澤昌文・小巻翔平・藤井保・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
  • Year and Date: 20131102-20131103
• [Presentation] 両生類モデル動物ネッタイツメガエル6系統における近交度及び遺伝的関係の解明 (2013)
  • Author(s): 渡辺愛・井川武・鈴木厚・柏木昭彦・倉林敦・藤井保・住田正幸
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
  • Year and Date: 20131102-20131103
• [Presentation] Evolutionary relationships and postmating isolation among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by mtDNA Cyt b gene sequences and crossing experiments. (2013)
  • Author(s): M Hasan・MM Islam・WMM Nalley・M Matsui・M Sumida
  • Organizer: 日本爬虫両生類学会第52回大会
  • Place of Presentation: 札幌市
  • Year and Date: 20131102-20131103
• [Presentation] 絶滅危惧種イボイモリの遺伝的多様性と飼育下繁殖 (2013)
  • Author(s): 住田正幸、井川武、菅原弘貴、田戸美雪、西谷琢磨、倉林敦、Mafizul Islam、大海昌平、勝連盛輝、藤井保
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山市
  • Year and Date: 20130926-20130928
• [Presentation] 東アジア産トノサマガエル種群の系統関係と遺伝子浸透 (2013)
  • Author(s): 小巻翔平・井川武・倉林敦・Min-Sook Min・Si-Min Lin・住田正幸
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山市
  • Year and Date: 20130926-20130928
• [Presentation] 両生類絶滅危惧種の飼育下繁殖による保全の試み (2013)
  • Author(s): 住田正幸、井川武、イスラム マフィズル、倉林敦、アラム シャフィクル、掛橋竜祐、田戸美雪、新谷望、菅原弘貴、西谷琢磨、大海昌平、勝連盛輝、藤井保
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 横浜市
  • Year and Date: 20130919-20130921
• [Presentation] アフリカツメガエルのゲノム倍数化過程に生じた染色体再配列と無尾両生類における性染色体の起源とその進化に関する分子細胞遺伝学的研究 (2013)
  • Author(s): 宇野好宣・西田千鶴子・高木知世・井川武・上野直人・住田正幸・松田洋一
  • Organizer: 日本遺伝学会第52回大会
  • Place of Presentation: 横浜市
  • Year and Date: 20130919-20130921
• [Presentation] Ion PGMを利用した両生類3種におけるマイクロサテライトマーカー開発 (2013)
  • Author(s): 井川武、小巻翔平、Nasrin Sultana、長岡麻衣、野澤昌文、Islam Mohammed Mafizul、大海昌平、住田正幸
  • Organizer: NGS現場の会第三回研究会
  • Place of Presentation: 神戸市
  • Year and Date: 20130904-20130905
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in Xenopus laevis after whole-genome duplication and the origin and evolution of sex chromosomes in anuran species. (2013)
  • Author(s): Uno, Y., Nishida, C., Takagi, C., Igawa, T., Ueno, N., Sumida, M., and Matsuda, Y.
  • Organizer: 19th International Chromosome Conference
  • Place of Presentation: Bologna, Italia
  • Year and Date: 20130902-20130906
• [Remarks] Takeshi Igawa Ph. D.
  • URL: https://sites.google.com/site/takeshi2igawa/