2013 Fiscal Year Final Research Report
Detecting tumor biomarker using aptamer-based highly-sensitive potentiometric biosensor
| Project/Area Number |
23750079
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
GODA Tatsuro 東京医科歯科大学, 生体材料工学研究所, 助教 (20588347)
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| Project Period (FY) |
2011 – 2013
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| Keywords | アプタマー / バイオマーカー / タンパク質 / 電界効果トランジスタ / バイオセンサー / 電位計測 / 自己組織化単分子膜 |
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| Research Abstract |
A highly sensitive detection of serum protein as a biomarker for cancer and/or tumor was conducted by using aptamer-based field-effect transistor (FET) biosensor without the need for labeling. The aptamer immobilized on the semiconductor-based biosensor specifically captured target protein within the electrical double layer which is spontaneously formed at the electrolyte/sensor interface. The FET sensor successfully detected the innate net-charge of the captured protein by the field effect. The technology allows a fast and low-cost detection of the biomarkers in a massively parallel format, providing a new platform for electrical sensing of proteins, proteomics, and post-translational modifications as an alternative way to conventional immuno-assays coupled with optical readout. The fundamental knowledge obtained by the project can be applied for clinical diagnosis for cancers, leading an innovation for current medical system.
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