A novel method for comprehensive detection of protein complexes in photosynthetic organisms

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: TAKABAYASHI Atsushi 北海道大学, 低温科学研究所, 助教 (90546417)
• Project Period (FY): 2011 – 2013
• Keywords: タンパク質複合体解析 / 光合成生物 / データベース

Research Abstract

To explore protein complexes systematically, we have tried to identify protein complexes by using the Blue-Native PAGE coupled with LC-MS/MS approach. We applied the approach into several photosynthetic organisms. Then, we constructed a new Web-based database PCoM-DB (http://pcomdb.lowtem.hokudai.ac.jp/proteins/top/) to make our data to public. Users can predict interaction partners with their proteins of interest through a user-friendly interface. I hope that the database to help users to find new protein complexes, which can contributes to understand cellular mechanisms in photosynthetic organisms.

Research Products

• [Journal Article] Functional analysis of light-harvesting-like protein 3 (LIL3) and its light-harvesting chlorophyll-binding motif in Arabidopsis (2014)
  • Author(s): Takahashi K, Takabayashi A, Tanaka A, and Tanaka R
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Pages: 987-999
  • Peer Reviewed
• [Journal Article] Evolutionary changes in chlorophyllide a oxygenase (CAO) structure contribute to the acquisition of a new light-harvesting complex in Micromonas (2013)
  • Author(s): Kunugi M, Takabayashi A, and Tanaka A
  • Journal Title: The Journal of Biological Chemistry Volume: 288 Pages: 19330-19341
  • Peer Reviewed
• [Journal Article] Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells (2013)
  • Author(s): Takabayashi A, Kadoya R, Kuwano M, Kurihara K, Ito H, Tanaka R, Tanaka A
  • Journal Title: SpringerPlus Volume: 2 Pages: 148
  • Peer Reviewed
• [Journal Article] The oligomeric states of photosystems and light-harvesting complexes in the chlorophyll b-less mutant (2011)
  • Author(s): Takabayashi A, Kuihara K, Kuwano M, Kasahara Y, Tanaka R, and Tanaka A
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 2103-2114
  • Peer Reviewed
• [Journal Article] Identification of the 7-hydroxymethyl chlorophyll a reductase of the chlorophyll cycle in Arabidopsis (2011)
  • Author(s): Megoro M, Ito H, Takabayashi A, Tanaka R, and Tanaka A
  • Journal Title: Plant Cell Volume: 23 Pages: 3442-3453
  • Peer Reviewed
• [Journal Article] Allocation of absorbed light energy in PSII to thermal dissipations in the presence or absence of PsbS subunits of Rice (2011)
  • Author(s): Ishida, S, Morita K, Kishine M, Takabayashi A, Murakami R, Takeda S, Shimamoto K, Sato F, and Endo T
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 1822-1831
  • Peer Reviewed
• [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的解析 (2012)
  • Author(s): 高林厚史、栗原克宜、田中亮一、田中歩
  • Organizer: 植物生理学会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] The application of BN-PAGE for comprehensive detection of the protein complexes in the chloroplast,シンポジウム「Basis, application and future of the blue-native PAGE in plant sciences」 (2011)
  • Author(s): Atsushi Takabayashi
  • Organizer: 植物生理学会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-20
• [Remarks] PCoM-DB
  • URL: http://pcomdb.lowtem.hokudai.ac.jp/proteins/top