Analysis of Plant Pathogen Resistance Supported by the ER Body

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: YAMADA Kenji 基礎生物学研究所, 高次細胞機構研究部門, 助教 (00360339)
• Project Period (FY): 2011 – 2012
• Keywords: 小胞体 / ERボディ / シロイヌナズナ / βグルコシダーゼ / 金属イオン輸送体 / 病原菌 / オルガネラ / 膜タンパク質

Research Abstract

Endoplasmic reticulum (ER) body is an ER derived structure that is accumulated in Arabidopsis thaliana. ER body is involved in plant defense system against insect and pathogen attack, since wound treatment induced ER bodies. Two soluble proteins NAI2 and PYK10, and two integral membrane proteins MEB1 and MEB2 are accumulated specifically in ER bodies, but their functions were obscure. I found that NAI2 and PYK10 are responsible for the ER body formation. MEB1 and MEB2 have iron/manganese transport activity, suggesting that ER body is involved in the metal stress resistance. These findings unrevealed unique mechanisms for ER body formation and function in plants

Research Products

• [Journal Article] Nishimura Proteomic analysis reveales that the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEX7 (Peroxin7) (2013)
  • Author(s): S.Cui, Y. Fukao, S. Mano, K. Yamada, M. Hayashi, M
  • Journal Title: J. Biol. Chem Volume: 288(8) Pages: 6014-6023
  • Peer Reviewed
• [Journal Article] Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. (2013)
  • Author(s): K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, M. Nishmura
  • Journal Title: Plant Physiol Volume: 161(1) Pages: 108-120
  • Peer Reviewed
• [Journal Article] A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots (2012)
  • Author(s): M. Nakayama, Y. Kaneko, Y. Miyazawa, N. Fujii, N. Higashitani, S. Wada, H. Ishida, K. Yoshimoto, K. Shirasu, K. Yamada, M. Nishimura, H. Takahashi
  • Journal Title: Planta Volume: 236(4) Pages: 999-1012
  • Peer Reviewed
• [Journal Article] Nishimura Unique defense strategy by the endoplasmic reticulum body in plants (2011)
  • Author(s): K. Yamada, I. Hara-Nishimura, M.
  • Journal Title: Plant Cell Physiol Volume: 52(12) Pages: 2039-2049
  • Peer Reviewed
• [Presentation] ERボディ特異的な二つの膜タンパク質(MEB1,MEB2)の解析 (2012)
  • Author(s): 山田健志,永野惇,仁科桃子,西村いくこ,西村幹夫
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学,京都
  • Year and Date: 20120316-18
• [Presentation] Rボディ成分による小胞体ドメインの分化がERボディ形成を誘導する (2011)
  • Author(s): 山田健志,永野惇,仁科桃子,西村いくこ,西村幹夫
  • Organizer: 第84回日本生化学会大会,国立京都国際会館
  • Place of Presentation: 京都
  • Year and Date: 20110921-24
• [Presentation] Differentiation of endoplasmic reticulum by ER-body luminal and membrane proteins on ER body formation (2011)
  • Author(s): K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, M. Nishimura
  • Organizer: Plant Biology 2011 (ASPP 2011 Annual Meeting)
  • Place of Presentation: Minneapolis Convention Center, Minneapolis, USA
  • Year and Date: 20110806-10
• [Remarks] ホームページ等 「シロイヌナズナのERボディに特異的な膜タンパク質を同定」
  • URL: http://www.nibb.ac.jp/pressroom/news/201
• [Remarks] 「小胞体のダイナミズムの解析」
  • URL: http://www.nibb.ac.jp/celmech/jp/Words/ERbody.html